(1) The amino acid sequence deduced from the nucleotide sequence contained both amino- and carboxyl-terminal sequences.
(2) The liver metastasis was produced by intrasplenic injection of the fluid containing of KATOIII in nude mouse and new cell line was established using the cells of metastatic site.
(3) In contrast, resting cells of strain CHA750 produced five times less IAA in a buffer (pH 6.0) containing 1 mM-L-tryptophan than did resting cells of the wild-type, illustrating the major contribution of TSO to IAA synthesis under these conditions.
(4) The nucleotide sequence of a 2.2-kb DNA fragment which contains the complete RAD7 gene was determined.
(5) As a consequence, similar response curves were obtained for urine specimens containing morphine or barbiturates.
(6) IT can, therefore, be excluded almost with certainty that the meat would contain such large amounts of hormone residues.
(7) The extent of the infectious process was limited, however, because the life span of the cultures was not significantly shortened, the yields of infectious virus per immunofluorescent cell were at all times low, and most infected cells contained only a few well-delineated small masses of antigen, suggestive of an abortive infection.
(8) In addition to oncogenes, the transferred DNA contains genes that direct the synthesis and exudation of opines, which are used as nutrients by the bacteria.
(9) These studies, in addition to demonstrating that the placenta contains TRH deamidase activity, suggest that losses of fetal TRH through the placenta are not large.
(10) Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive.
(11) The human placental villus tissue contains opioid receptors and peptides.
(12) Gel filtration of the 40,000 rpm supernatant fraction of a homogenate of rat cerebral cortex on a Sepharose 6B column yielded two fractions: fraction II with the "Ca(2+) plus Mg(2+)-dependent" phosphodiesterase activity and fraction III containing its modulator.
(13) These cells contained organelles characteristic of the maturation stage ameloblast and often extended to the enamel surface, suggesting a possible origin from the ameloblast layer.
(14) It includes preincubation of diluted plasma with ellagic acid and phospholipids and a starting reagent that contains calcium and a chromogenic peptide substrate for thrombin, Tos-Gly-Pro-Arg-pNA.
(15) The p60v-src protein encoded by Prague Rous sarcoma virus was found to contain two sites of tyrosine phosphorylation.
(16) The 68C intermolt puff of Drosophila melanogaster contains a cluster of three glue protein genes, Sgs-3, Sgs-7, and Sgs-8.
(17) The deduced amino acid sequence contained no consensus sequence indicative of N-glycosylation.
(18) Maximal yields of lipid and aflatoxin were obtained with 30% glucose, whereas mold growth, expressed as dry weight, was maximal when the medium contained 10% glucose.
(19) This analysis demonstrated that more than 75% of cosmids containing a rare restriction site also contained a second rare restriction site, suggesting a high degree of CpG-rich restriction site clustering.
(20) The region containing the injection stop signal (iss) has been cloned and sequenced and found to contain numerous large repeats and inverted repeats which may be part of the iss.
Nef
Definition:
(n.) The nave of a church.
Example Sentences:
(1) However, an anti-nef antibody response was also seen in 5 of 93 (5%) nonrisk dermatological patients and in 4 of 37 (11%) healthy blood donors.
(2) Plasma was analyzed for NEF and 3 metabolites [hydroxynefazodone (HO-NEF), m-chlorophenylpiperazine (mCPP) and p-hydroxynefazodone (p-HO-NEF)] by a validated HPLC assay.
(3) We found that HIV Nef shares sequence and structural features with scorpion peptides known to interact with K+ channels.
(4) These observations were confirmed by in vitro experiments using purified NeFs.
(5) DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef.
(6) Complement-independent binding of C3 nephritic factor (NEF) to sheep erythrocytes was observed in heat-inactivated sera from patients having this autoantibody.
(7) In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1.
(8) Human T-lymphocytic cell line H9 infected with the HTLV-IIIB isolate of human immunodeficiency virus type 1 (HIV-1) synthesizes two forms of the Nef protein (p25 and p27) that differ both in molecular weight and charge.
(9) The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography.
(10) Earlier studies indicated nef to be a negative regulatory factor for viral transcription, whereas more recent studies report evidence against this original hypothesis.
(11) Absence of anti-nef antibodies was associated with symptomatic HIV infection.
(12) The effects of Nef on the transmembrane potential were studied in rabbit sinoatrial nodes and the clusters of cultured cardiac myocytes from neonatal rats.
(13) The type 1 human immunodeficiency virus (HIV-1) encodes a 27-kDa protein termed Nef (negative factor).
(14) Surprisingly, CTL specific for regulatory, non-structural nef protein appear to be a major constituent of the human immune response to HIV.
(15) Determination of the B-cell epitopes of the nef molecule encoded by the human immunodeficiency virus type 1 (HIV-1) was undertaken using a set of six synthetic peptides.
(16) In contrast, the extent and the appearance of nef-specific antibodies during the course of infection varied considerably between individual animals.
(17) The functions of yet other viral genes (nef, vpt) remain unclear.
(18) All of these anti-nef positive sera also contained antibodies directed against viral structural proteins.
(19) The results showed that the vascular selectivity values of Ver, Nef, and Tet were 1.15, 0.32, and 0.20, respectively in WKY and 0.80, 0.24, and 0.10, respectively in SHR.
(20) Recombinant antigens containing all or parts of the HIV-1 proteins p24, Nef and gp41 and HIV-2 gp36 have been purified and used to develop a rapid immunoassay to detect and differentiate between HIV-1 and HIV-2 antibodies in a single test.