(1) When micF was cloned into a high-copy-number plasmid it repressed ompF gene expression, whereas when cloned into a low-copy-number plasmid it did not.
(2) On removal of selective pressure, the His+ phenotype was lost more readily than the Ura+ Trp+ markers, with a corresponding decrease in plasmid copy number.
(3) We have generated a series of mutants in the two copies of this motif present in human immunodeficiency virus type 1.
(4) The v-erb A oncogene of avian erythroblastosis virus is a mutated and virally transduced copy of a host cell gene encoding a thyroid hormone receptor.
(5) The fact that the security service was in possession of and retained the copy tape until the early summer of 1985 and did not bring it to the attention of Mr Stalker is wholly reprehensible,” he wrote.
(6) A method for constructing Ti plasmids bearing multiple copies of a sequence integrated in tandem is described.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) This 54-bp fragment is present at about 2000-2500 copies in the bovine male genome.
(9) Construction of a repR-lacZ fusion proved that the increase in copy number was due to a proportional increase in the amount of RepR protein.
(10) The E2A mutants were propagated by growth in human cell lines which express an integrated copy of the DBP gene under the control of a dexamethasone-inducible promoter (D. F. Klessig, D. E. Brough, and V. Cleghon, Mol.
(11) The fusion was prepared in multicopy (pVLN102 plasmid) and low-copy-number states, the latter constructed as a lambda phage lysogen carrying a fur'-'lacZ insert.
(12) An expanded version of this paper, containing full experimental details of the semisynthesis and characterization of [GlyA1-3H]insulin, has been deposited as Supplementary Publication SUP 50129 (30 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem.
(13) All 51 undifferentiated NPCs contained significant numbers of EBV-genome copies per cell.
(14) By way of encouragement we've got 10 copies of Faber's smart new anniversary edition to give away.
(15) Programmed gene rearrangements are used in nature to to alter gene copy number (gene amplification and deletion), to create diversity by reassorting gene segments (as in the formation of mammalian immunoglobulin genes), or to control the expression of a set of genes that code for the same function (such as surface antigens).
(16) All three units are present in the same, probably single, copy number.
(17) Some derivatives of pIJ101, a 8.9 kb Streptomyces multi-copy plasmid, can co-exist with each other at similar copy numbers but others are strongly incompatible.
(18) Genomic southern hybridization experiments clearly indicate that the ribosomal RNA genes are unique single-copy DNA in H. cutirubrum.
(19) We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit.
(20) There are approximately 20 copies of Tc1(Hin) amongst the Tc1's present in the Bergerac genome.
Triplicate
Definition:
(v. t.) Made thrice as much; threefold; tripled.
(n.) A third thing corresponding to two others of the same kind.
Example Sentences:
(1) Proton induced X-ray emission (PIXE) analyses were performed on single hair fibers in triplicate from 103 individuals in order to determine sulfur, zinc, calcium, and chlorine content.
(2) One hundred isolates were tested in triplicate, and geometric mean MICs were plotted against arithmetic mean zone diameters and regression statistics calculated.
(3) All mixtures were prepared in triplicate and added with water.
(4) Nine gamma gene triplications from seven ethnic groups were analyzed for G gamma and hemoglobin F (Hb F) values of heterozygotes and for the presence of polymorphic XmnI restriction sites 5' to the gamma genes.
(5) A unique triplication of 108 bp, including the evolutionary conserved sequence CSB-3, was found.
(6) The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma.
(7) Each experiment was made in triplicate, the vaporizer being dried out between each.
(8) Over a period of sixty hours, no relevant influence on sperm motility in comparison to control media was noted on triplicate testing.
(9) Both sides of bilateral ureteral triplications in our case belonged to type B of Smith's classification.
(10) All patients with triplicated pouches and all except one with duplicated pouches were able to defaecate spontaneously, without needing to intubate the reservoir.
(11) The presence of the gamma-globin gene triplication was frequently observed in M. fuscata.
(12) One baby was heterozygous for a chromosome with two linked G gamma globin genes (-G gamma-G gamma-delta-beta), which has also been observed in Black newborns [Powers et al, Nucleic Acids Res 12:7023, 1984], while the two other babies were heterozygous for a chromosome with triplicated gamma globin genes, presumably of the -G gamma-A gamma G gamma-A gamma-delta-beta arrangement.
(13) This method allows assays of opsonic activity to be performed in triplicate on large numbers of sera with a relatively small number of phagocytes and should aid the investigation of the role of opsonisation in infectious disease.
(14) Ureteral triplication is a rare anomaly of the urinary tract.
(15) The optimal disk concentration and incubation time were determined by utilizing triplicate sets of quadrant plates prepared with supplemented brucella (Difco) blood agar and swabbed with a 24-h broth (BBL; 135 C thioglycolate) suspension of the test organism.
(16) Three bags each were stored at 4, 20, and 37 degrees C; three 20-mL samples were removed from each bag after 0, 0.5, 1, 2, 5, 7, 10, 14, 21, 28, and 42 days and analyzed in triplicate by high-performance liquid chromatography.
(17) The structure resulting from this triplication consisted of three large loops and three long connecting segments.
(18) Run-to-run assay CV was 6% for primidone and phenobarbital and 13% for phenytoin, based on the means of triplicate determinations of a sample with a mid-range concentration.
(19) All measurements were made in triplicate and averaged.
(20) The different cytogenetic mechanism given in these cases shows us that with independence of it, it exists a triplication of the half distal short arm of chromosome 9 which gives specificity to these phenotypic features.