(1) When micF was cloned into a high-copy-number plasmid it repressed ompF gene expression, whereas when cloned into a low-copy-number plasmid it did not.
(2) On removal of selective pressure, the His+ phenotype was lost more readily than the Ura+ Trp+ markers, with a corresponding decrease in plasmid copy number.
(3) We have generated a series of mutants in the two copies of this motif present in human immunodeficiency virus type 1.
(4) The v-erb A oncogene of avian erythroblastosis virus is a mutated and virally transduced copy of a host cell gene encoding a thyroid hormone receptor.
(5) The fact that the security service was in possession of and retained the copy tape until the early summer of 1985 and did not bring it to the attention of Mr Stalker is wholly reprehensible,” he wrote.
(6) A method for constructing Ti plasmids bearing multiple copies of a sequence integrated in tandem is described.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) This 54-bp fragment is present at about 2000-2500 copies in the bovine male genome.
(9) Construction of a repR-lacZ fusion proved that the increase in copy number was due to a proportional increase in the amount of RepR protein.
(10) The E2A mutants were propagated by growth in human cell lines which express an integrated copy of the DBP gene under the control of a dexamethasone-inducible promoter (D. F. Klessig, D. E. Brough, and V. Cleghon, Mol.
(11) The fusion was prepared in multicopy (pVLN102 plasmid) and low-copy-number states, the latter constructed as a lambda phage lysogen carrying a fur'-'lacZ insert.
(12) An expanded version of this paper, containing full experimental details of the semisynthesis and characterization of [GlyA1-3H]insulin, has been deposited as Supplementary Publication SUP 50129 (30 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem.
(13) All 51 undifferentiated NPCs contained significant numbers of EBV-genome copies per cell.
(14) By way of encouragement we've got 10 copies of Faber's smart new anniversary edition to give away.
(15) Programmed gene rearrangements are used in nature to to alter gene copy number (gene amplification and deletion), to create diversity by reassorting gene segments (as in the formation of mammalian immunoglobulin genes), or to control the expression of a set of genes that code for the same function (such as surface antigens).
(16) All three units are present in the same, probably single, copy number.
(17) Some derivatives of pIJ101, a 8.9 kb Streptomyces multi-copy plasmid, can co-exist with each other at similar copy numbers but others are strongly incompatible.
(18) Genomic southern hybridization experiments clearly indicate that the ribosomal RNA genes are unique single-copy DNA in H. cutirubrum.
(19) We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit.
(20) There are approximately 20 copies of Tc1(Hin) amongst the Tc1's present in the Bergerac genome.
Duplicating
Definition:
(p. pr. & vb. n.) of Duplicate
Example Sentences:
(1) Short incubations with heparin (5 min) caused a release of the enzyme into the media, while longer incubations caused a 2-8-fold increase in net lipoprotein lipase secretion which was maximal after 2-16 h depending on cell type, and persisted for 24 h. The effect of heparin was dose-dependent and specific (it was not duplicated by other glycosaminoglycans).
(2) Preliminary data also suggest that high-molecular-weight rearrangements of the duplicated region are present in all tissues.
(3) In the second comparison, HSV was isolated from 225 of 1,026 (21.9%) specimens and duplicate human foreskin fibroblast cell wells stained at 24 and 72 h were PAP positive in 241 of 1,026 (23.5%).
(4) Evidence reported here shows that, consistent with prediction, 10 carcinogens are all active in inducing tandem duplications.
(5) So we concluded that duplications and accessories should be thought to have similar meanings with the ordinary branching patterns of MCA in the occurrence of aneurysms.
(6) The 500-bp element arose by duplication of one half of a 180-bp ancestor and insertion of a foreign segment between the two duplicated parts followed by amplification.
(7) A case of incomplete peno-scrotal transposition, with a perineal anorectal duplication, vesico-ureteric reflux and thoracic hemivertebrae is presented.
(8) For the case of the fluctuating pressure, the strength of the artery becomes considerably lower than those under constant amplitude and two-step-multi-duplicated pulsatile pressure.
(9) Reciprocal translocations involving the short arm of acrocentric chromosomes can segregate to produce partial duplications without associated deletions.
(10) The authors report a case of total bladder duplication by frontal septum.
(11) Control-operated cells with centrosomes left in the karyoplast progress through the cell cycle, duplicate the centrosome, and form clonal cell colonies.
(12) Partial duplication of the proximal part of the long arm of chromosome 5, on the other hand, is associated mainly with musculoskeletal abnormalities including muscle hypotrophy and hypotonia, scoliosis, lordosis, pectus carinatum, cubitus valgus, and genu valgum, in addition to psychomotor retardation.
(13) Using fluorescent in situ hybridization and digital imaging microscopy, we mapped probe p32.1 (D11S16) to the proximal part of region 11p14 (11p14.1) and demonstrated duplication of this probe in our patient.
(14) The efflux rate for EB of strains with duplicated ebr genes was twice the rate of strains with a single ebr gene.
(15) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(16) The 3' untranslated region of the VMRI gene 11 equivalent contains a clear duplication of a portion of its coding sequence.
(17) The regulatory region of the casein gene contains two different TATA signals flanking the duplication site in the promoter region.
(18) A 68-year-old female patient was admitted for the examination of duplication of right ureter and right hydronephrosis.
(19) The curiously double nature of the virgin in this tale, her purity versus her duplicity, seems unquestionably related to the infantile split mother, as elucidated by Klein--a connection explored in an earlier paper.
(20) Furthermore, duplications in the vicinity of this locus involving the beta-amyloid gene and the proto-oncogene ets-2 have been reported in association with AD.