(1) When micF was cloned into a high-copy-number plasmid it repressed ompF gene expression, whereas when cloned into a low-copy-number plasmid it did not.
(2) On removal of selective pressure, the His+ phenotype was lost more readily than the Ura+ Trp+ markers, with a corresponding decrease in plasmid copy number.
(3) We have generated a series of mutants in the two copies of this motif present in human immunodeficiency virus type 1.
(4) The v-erb A oncogene of avian erythroblastosis virus is a mutated and virally transduced copy of a host cell gene encoding a thyroid hormone receptor.
(5) The fact that the security service was in possession of and retained the copy tape until the early summer of 1985 and did not bring it to the attention of Mr Stalker is wholly reprehensible,” he wrote.
(6) A method for constructing Ti plasmids bearing multiple copies of a sequence integrated in tandem is described.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) This 54-bp fragment is present at about 2000-2500 copies in the bovine male genome.
(9) Construction of a repR-lacZ fusion proved that the increase in copy number was due to a proportional increase in the amount of RepR protein.
(10) The E2A mutants were propagated by growth in human cell lines which express an integrated copy of the DBP gene under the control of a dexamethasone-inducible promoter (D. F. Klessig, D. E. Brough, and V. Cleghon, Mol.
(11) The fusion was prepared in multicopy (pVLN102 plasmid) and low-copy-number states, the latter constructed as a lambda phage lysogen carrying a fur'-'lacZ insert.
(12) An expanded version of this paper, containing full experimental details of the semisynthesis and characterization of [GlyA1-3H]insulin, has been deposited as Supplementary Publication SUP 50129 (30 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem.
(13) All 51 undifferentiated NPCs contained significant numbers of EBV-genome copies per cell.
(14) By way of encouragement we've got 10 copies of Faber's smart new anniversary edition to give away.
(15) Programmed gene rearrangements are used in nature to to alter gene copy number (gene amplification and deletion), to create diversity by reassorting gene segments (as in the formation of mammalian immunoglobulin genes), or to control the expression of a set of genes that code for the same function (such as surface antigens).
(16) All three units are present in the same, probably single, copy number.
(17) Some derivatives of pIJ101, a 8.9 kb Streptomyces multi-copy plasmid, can co-exist with each other at similar copy numbers but others are strongly incompatible.
(18) Genomic southern hybridization experiments clearly indicate that the ribosomal RNA genes are unique single-copy DNA in H. cutirubrum.
(19) We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit.
(20) There are approximately 20 copies of Tc1(Hin) amongst the Tc1's present in the Bergerac genome.
Replication
Definition:
(n.) An answer; a reply.
(n.) The reply of the plaintiff, in matters of fact, to the defendant's plea.
(n.) Return or repercussion, as of sound; echo.
(n.) A repetition; a copy.
Example Sentences:
(1) In contrast, DNA polymerase alpha, the enzyme involved in chromosomal DNA replication, was relatively insensitive to CA1.
(2) Assessment of the likelihood of replication in humans has included in vitro exposure of human cells to the potential pesticidal agent.
(3) Apparently, the irradiation with visible light of a low intensity creates an additional proton gradient and thus stimulates a new replication and division cycle in the population of cells whose membranes do not have delta pH necessary for the initiation of these processes.
(4) In each study, all subjects underwent four replications (over two days) of one of the six permutations of the three experimental conditions; each condition lasted 5 min.
(5) It has also been used to measure the amount of excision repair performed by non-replicating cells damaged by carcinogens.
(6) PMN were found to be nonpermissive for HSV replication and were unable to bind virus in the absence of antibody.
(7) However, further improvement of culture systems is needed for active replication of HBV in vitro.
(8) Businesses fleeing Brexit will head to New York not EU, warns LSE chief Read more Amid attempts by Frankfurt, Paris and Dublin to catch possible fallout from London, Sir Jon Cunliffe said it was highly unlikely that any EU centre could replicate the services offered by the UK’s financial services industry.
(9) It is concluded that fibroblast replication is an important mechanism leading to the pathologic fibrosis seen in graft versus host disease and, by analogy, probably other types of immunologically mediated fibrosis.
(10) We found that, although controlled release delivery of ddC inhibited de novo FeLV-FAIDS replication and delayed onset of viremia when therapy was discontinued (after 3 weeks), an equivalent incidence and level of viremia were established rapidly in both ddC-treated and control cats.
(11) None of the compounds proved active against the replication of retroviruses (human immunodeficiency virus, murine sarcoma virus) at concentrations that were not toxic to the host cells.
(12) The M 13 specific DNA present in minicells isolated several hours after infection consists of single stranded viral DNA and double stranded replicative forms in nearly equal amounts.
(13) A standard protocol is reported for the highly efficient demonstration of replication patterns corresponding to R-type and G-type banding.
(14) Infidelity of replication is a hallmark of the HIV-1 RT, and replication errors by the enzyme on RNA and DNA templates are discussed.
(15) To determine if late viral genes contribute to target cell lysis, phosphonoacetic acid (PAA), an inhibitor of DNA polymerase activity, was used to block DNA replication that is required for expression of late viral proteins.
(16) Virus replication in nasal turbinates was not diminished while infection in the lung was suppressed sufficiently for the infected mice to survive the infection.
(17) The specificity of vaccinia restriction was demonstrated by the ability of myxoma virus to replicate in nonimmune and vaccinia-immune macrophages.
(18) After permeabilization, with attendant partial extraction, the preparation can be fixed, then viewed by either deep-etch replication, or by high-resolution scanning electron microscopy, with structure of interest revealed in deep view.
(19) The model is based on the concept that a cell with hypothetically unlimited replicative potential--i.e.
(20) However, the degree of inhibition of parasite replication after exposure to rMu-GM-CSF was not as great as after treatment with rMu-IFN-gamma, and much more rMu-GM-CSF than rMu-IFN-gamma was required to achieve an equivalent antimicrobial effect.