(1) After stimulation with lipopolysaccharide and calcium ionophore A23187, culture supernatants of clones c18A and c29A showed cytotoxic activity against human melanoma A375 Met-Mix and other cell lines which were resistant to the tumor necrosis factor, lymphotoxin and interleukin 1.
(2) If ascorbic acid was omitted from the culture medium, the extensive new connective tissue matrix was not produced.
(3) He is also the foremost theorist of the Tijuana-San Diego border in terms of what happens when the urban culture of the developing world collides with that of the developed world.
(4) Our data suggest that a rational use of surveillance cultures and serological tests may aid in an earlier diagnosis of FI in BMT patients.
(5) In attacking the motion to freeze the licence fee during today's Parliamentary debate the culture secretary, Andy Burnham, criticised the Tory leader.
(6) The extent of the infectious process was limited, however, because the life span of the cultures was not significantly shortened, the yields of infectious virus per immunofluorescent cell were at all times low, and most infected cells contained only a few well-delineated small masses of antigen, suggestive of an abortive infection.
(7) It involves creativity, understanding of art form and the ability to improvise in the highly complex environment of a care setting.” David Cameron has boosted dementia awareness but more needs to be done Read more She warns: “To effect a cultural change in dementia care requires a change of thinking … this approach is complex and intricate, and can change cultural attitudes by regarding the arts as central to everyday life of the care home.” Another participant, Mary*, a former teacher who had been bedridden for a year, read plays with the reminiscence arts practitioner.
(8) Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive.
(9) Patient or fetal cord serum is commonly used as a protein supplement to culture media used in in-vitro fertilization (IVF).
(10) The rate of accumulation of degraded LDL products was lower in collagen gel cultures, but the final levels achieved were the same in the two substrata.
(11) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
(12) A simple method for ultrarapid freezing of cell cultures in monolayers was developed.
(13) The results indicate that OA-bearing macrophages primed T cells and generated helper T cells, whereas the culture of normal lymphocytes with soluble OA in the absence of macrophages generated suppressor T cells.
(14) The effects of phenoxyacetic acid herbicides were investigated on the induction of chromosome aberrations in human peripheral lymphocyte cultures in vitro and in lymphocytes of exposed workers in vivo.
(15) Small pieces of anterior and posterior quail wing-bud mesoderm (HH stages 21-23) were placed in in vitro culture for up to 3 days.
(16) Human gingival fibroblasts were allowed to attach and spread on bio-glasses for 1-72 h. Unreactive silica glass and cell culture polystyrene served as controls.
(17) However, further improvement of culture systems is needed for active replication of HBV in vitro.
(18) This paper has considered the effects and potential application of PFCs, their emulsions and emulsion components for regulating growth and metabolic functions of microbial, animal and plant cells in culture.
(19) Cells (1 x 10(5)) were seeded in 12- x -75-mm tissue culture tubes and incubated with various doses of IL-1 beta, IL-1 alpha, TNF-alpha, and IFN-gamma, alone or in specific combinations, for 15 min, two, 12, 24, and 72 h. PGE concentrations in the media were measured by radio-immunoassay.
(20) This activation demonstrated in humans confirms the pharmacological results of the interferon induction obtained with SL04 in vivo in mice and in vitro in human cell cultures.
Isolation
Definition:
(n.) The act of isolating, or the state of being isolated; insulation; separation; loneliness.
Example Sentences:
(1) A series of human cDNA clones of various sizes and relative localizations to the mRNA molecule were isolated by using the human p53-H14 (2.35-kilobase) cDNA probe which we previously cloned.
(2) On the other hand, human IL-9, which is a homologue to murine P40, was cloned from a cDNA library prepared with mRNA isolated from PHA-induced T-cell line (C5MJ2).
(3) Open field behaviors and isolation-induced aggression were reduced by anxiolytics, at doses which may be within the sedative-hypnotic range.
(4) The ability of azelastine to influence antigen-induced contractile responses (Schultz-Dale phenomenon) in isolated tracheal segments of the guinea-pig was investigated and compared with selected antiallergic drugs and inhibitors of arachidonic acid metabolism.
(5) One thing seems to be noteworthy in their opinion: the bacterial resistance of the germs isolated from the urine is bigger than the one of the germs isolated from the respiratory apparatus.
(6) Thus, saponin and ammonium chloride can be used to isolate whole infected erythrocytes, depleted of hemoglobin, by selective disruption of uninfected cells.
(7) Detergent-solubilized HLA antigens were isolated from a human lymphoblastoid cell using an anti-beta2-microglobulin immunoaffinity column.
(8) A phytochemical investigation of an ethanolic extract of the whole plant of Echites hirsuta (Apocynaceae) resulted in the isolation and identification of the flavonoids naringenin, aromadendrin (dihydrokaempferol), and kaempferol; the coumarin fraxetin; the triterpene ursolic acid; and the sterol glycoside sitosteryl glucoside.
(9) The present findings indicate that the deafferented [or isolated] hypothalamus remains neuronally isolated from the environment if the operation is carried out later than the end of the first week of life.
(10) Proliferation assays using F3 showed that 15 (14 CD4+ and 1 CD8+) of the 18 isolated clones were specific for T. gondii.
(11) Similar to intact crayfish, animals with an isolated protocerebrum-eyestalk complex, exhibit competent circadian rhythms in the electroretinogram (ERG).
(12) Reiteration VII (within protein coding regions of genes US10 and US11) and reiteration IV (within introns of genes US1 and US12) were stable between the isolates (group 1).
(13) The role of O2 free radicals in the reduction of sarcolemmal Na+-K+-ATPase, which occurs during reperfusion of ischemic heart, was examined in isolated guinea pig heart using exogenous scavengers of O2 radicals and an inhibitor of xanthine oxidase.
(14) The agent present in the serum which causes dissolution of the fibrin clot was isolated and identified as pepsinogen.
(15) Completeness of isolation of the coronary and systemic circulations was shown by the marked difference in appearance times between the reflex hypotensive responses from catecholamine injections into the isolated coronary circulation and the direct hypertensive response from a similar injection when the circulations were connected as well as by the marked difference between the pressure pulses recorded simultaneously on both sides of the aortic balloon separating the two circulations.4.
(16) At the same time the duodenum can be isolated from the stomach and maintained under constant stimulus by a continual infusion at regulated pressure, volume and temperature into the distal cannula.
(17) Only the approximately 2.7 kb mRNA species was visualized in Northern blots of total cellular and poly(A+) RNA isolated from cardiac ventricular muscle.
(18) These major departmental transformations are being run in isolation from each other.
(19) This postulate is supported by a limited study of the serovars present among the isolates.
(20) Plasma membranes were isolated from rat kidney and their transport properties for sodium, calcium, protons, phosphate, glucose, lactate, and phenylalanine were investigated.