(n.) The substance which, added to the material of a cell wall, makes it waterproof, as in cork.
Example Sentences:
(1) Using tritiated apple cutin as substrate, the two cutinases showed similar substrate concentration dependence, protein concentration dependence, time course profiles, and pH dependence profiles with optimum near 10.0.
(2) Using unlabeled cutin, the rate of dihydroxyhexadecanoic acid release from apple fruit cutin by cutinase I was determined to be 4.4 mumol per min per mg.
(3) 7) capable of degrading cutin, the insoluble lipid-polyester matrix covering the surface of plants, and hydrolysing triglycerides.
(4) Both mRNA and protein were inducible by cutin hydrolysate, while hypovirulence agents suppressed the level of mRNA and the enzyme.
(5) Transformants with a disrupted CUT1 gene failed to produce a cutin-inducible esterase that is normally detected by activity staining on non-denaturing polyacrylamide gels.
(6) The seed coat of almond (Prunus amygdalus Batsch) contains up to 30% procyanidins with different degrees of polymerisation and, in addition, fatty oils, lignin, polysaccharides and cutin.
(7) omega-Hydroxy fatty acids were more effective in inducing cutinase than any of the other more polar acids of cutin.
(8) A radial immunodiffusion assay for cutinase was developed, and the induction of cutinase by cutin hydrolysate was confirmed by this direct assay.
(9) Thus, the high resistance of plant cuticles to transport of 2,4-D can be attributed to both low diffusion and partition coefficients in the transport-limiting layer made up of cutin and soluble lipids which are densely packed and highly ordered.
(10) Induction of cutinase by cutin or hydrolyzed cutin after growth on glucose medium was similarly reduced.
(11) The mutant possessed an 80 to 90% reduction in cutinase activity when grown for 3 to 5 days on acetate- or cutin-containing medium.
(12) Thin-layer chromatographic analysis of the products released from labeled apple fruit cutin showed that the extracellular enzyme released all classes of cutin monomers.
(13) However, unlike the previously studied fungal systems, cutin hydrolysate did not induce cutinase.
(14) From a physiological point of view, peroxygenase and this newly described epoxide hydrolase could be responsible, in vivo, for the biosynthesis of a class of oxygenated fatty acid compounds known to be involved in cutin monomers production and in plant defense mechanisms.
(15) Hygromycin-resistant transformants of F. solani pisi generated by electroporation were assayed for CAT activity inducible by cutin hydrolysate and for glucose repression of this induction.
(16) These results suggest that cutin monomer causes phosphorylation of a transcription factor that binds to the -225 to -360 segment of the cutinase gene and enhances transcription of this gene.
(17) Non-denaturing polyacrylamide gels stained for esterase activity show a single major band among intracellular and extracellular proteins from cutin-grown cultures that is not present among intracellular and extracellular proteins prepared from glucose-grown or carbon-starved cultures.
(18) The gene is expressed when cutin is the sole carbon source but not when the carbon source is cutin and glucose together or glucose alone.
(19) This demonstrates that permeance is determined by the soluble cuticular lipids associated with the cutin, rather than by cutin alone.
(20) The properties of the homogeneous cutinase I, cutinase II, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown Fusarium solani F. pisi (R.E.