(1) It is theoretically considered that like glycation, carbamylation may occur between cyanic acid and not only Hb but also various peptides and proteins.
(2) The toxical potentiality of the cyanic radicle and the detoxicating characteristic of the amide group are studied following the evolution of the benzonitrile and benzamide toxicity when a cyanic or amide radicle is added to the original molecule.
(3) The rate of reaction between alpha-amino groups and cyanic acid was followed at 26 degrees and ionic strength 0.2 M as a function of pH of human hemoglobin Ao solutions to determine the pK and the pH-independent second order rate constant, kappa, for these groups in the alpha and beta chains.
(4) It has been demonstrated that the binding of cyanic acid, formed from urea which is increased in renal failure, to hemoglobin (Hb) results in the formation of HbA1.
(5) The combination of Hb and cyanic acid is called carbamylation, a nonspecific reaction between protein and cyanic acid.
(6) A nonspecific binding reaction between cyanic acid formed from urea and protein or peptide is called carbamylation.
(7) It is the respective localization of the cyanic or amide radicles which appears to play the most important role.
Cyanogen
Definition:
(n.) A colorless, inflammable, poisonous gas, C2N2, with a peach-blossom odor, so called from its tendency to form blue compounds; obtained by heating ammonium oxalate, mercuric cyanide, etc. It is obtained in combination, forming an alkaline cyanide when nitrogen or a nitrogenous compound is strongly ignited with carbon and soda or potash. It conducts itself like a member of the halogen group of elements, and shows a tendency to form complex compounds. The name is also applied to the univalent radical, CN (the half molecule of cyanogen proper), which was one of the first compound radicals recognized.
Example Sentences:
(1) Uptake into arterial collagens and release of labeled hydroxyproline were then measured after 2.5 h. The relative amounts of types I and III collagens were assessed from the levels of cyanogen-bromide-derived peptides alpha 1(I)CB8 and alpha 1(III)CB5, respectively, after sodium dodecyl sulfate polyacrylamide gel electrophoresis.
(2) The use of SDS-polyacrylamide gel electrophoresis of the cyanogen bromide derived peptides from fibrous cartilage collagens enabled to calculate type I to type II collagen ratio in this tissue.
(3) The sequence was obtained from analysis of peptides isolated from chymotryptic, cyanogen bromide, and limited acid cleavages of the protein.
(4) Cyanogen bromide cleavage of the 145000 subunit produced a single peptide of Mr 75000.
(5) The amino acid sequence of a brain-specific protein, S-100, has been determined by the analysis of four cyanogen bromide peptides and fourteen tryptic peptides derived from the protein.
(6) The mapping of the MAb epitopes was also facilitated by immunoblotting analysis of HlyA polypeptide fragments derived by cyanogen bromide cleavage.
(7) The heavy chain of a pathological immunoglobulin G (Daw) of type L, subclass gamma(2b) (We) and Gm(a+)(f-), has been cleaved with cyanogen bromide.
(8) Purified boveine growth hormone labeled enzymatically with iodine-125 was covalently coupled to cyanogen bromide activated Sepharose 4B gel and to diazotized zirconia-clad glass beads.
(9) This transformation is consistent with the general pathway for cyanogenic glycoside biosynthesis proposed on the basis of in vivo experiments.
(10) A covalent binding of transketolase to bromo-cyanogen-activated Sepharose 4B at the pH value of the maximum activity and stability of the enzyme equal to 7,6 has been carried out.
(11) By using synthetic peptides corresponding to human and murine protein AA segments and cyanogen bromide fragments of human protein AA, we show evidence that the amyloidogenic part of the molecule is the first 10-15 amino acid long segment.
(12) Dot-blot analysis indicated that antibody 47N3-6 could bind to a fragment, KRKRSQHse, derived from residues 132-137 of rHuIFN gamma-A, but could bind only weakly to the cyanogen bromide fragment corresponding to residues 138-146.
(13) Two primary sets of overlapping fragments were obtained by cleavage of the S-carboxymethylated protein at methionyl and lysyl bonds following treatment with cyanogen bromide and Achromobacter protease I, respectively.
(14) All seven cyanogen bromide peptides were isolated in pure form and were ordered by peptides isolated from tryptic digests.
(15) Competitive enzyme-linked immunosorbent assay experiments using peptide-specific antibodies against cyanogen bromide fragments generated from human dihydrofolate reductase show that the binding of folate, NADPH, and methotrexate, either in binary or in ternary complexes with the wild-type enzyme, causes a striking reduction in the binding of the antibodies.
(16) Cyanogen bromide cleavage peptides showed appropriate collagen formed by unstressed autogenous tendon transplants removed from rabbit knees up to 2 years from transplantation.
(17) Using oligonucleotide probes derived from the protein sequence of a cyanogen bromide fragment, we have cloned the gene for P.69 from B. pertussis CN2992.
(18) Antisera to extracts of a variety of bronchogenic carcinoma were raised in rabbits and extensively absorbed with immunoadsorbents prepared with normal lung extracts cyanogen bromide linked to Sepharose 4B, and glutaraldehyde insolubilized normal lung extracts.
(19) The labeled site not blocked by cAMP is also contained in a Mr = 28,000 cyanogen bromide fragment of the enzyme that does not overlap with the Mr = 36,000 proteolytic fragment.
(20) Verification that the deduced primary structure was that of p190 was demonstrated through microsequence analysis of a cyanogen bromide peptide generated from chick brain p190.