(1) In general, optimal DAGAT activity in vitro was observed when long-chain unsaturated acyl-CoAs and diacylglycerols (DAGs) containing long acyl chains were used as substrates for in vitro TAG synthesis (although 1,2-didecanoin was also very effective).
(2) Incubation of microsomes with CDP-DAG of different fatty acid composition results in quantitative and qualitative differences in lysoPI formation.
(3) We now report that two synthetic diacylglycerols (DAG) replicate the stimulatory and inhibitory effects of TPA on frog skin.
(4) The distribution of PKC returned to control values by 24 h. High glucose did not stimulate phosphoinositide hydrolysis, as evidenced by the absence of an increase in the water-soluble inositol phosphates, indicating that DAG was not generated through the action of a phosphoinositide-specific phospholipase C. Cells treated with the cell-permeable DAG analogue 1-oleoyl-2-acetyl glycerol to activate PKC displayed approximately two-fold increases of fibronectin, laminin, and type IV collagen mRNA levels after normalization against actin.
(5) We observed a 30-45% increase in DAG in rat gastrocnemius and diaphragm muscles, 5-15 min after intramuscular or intravenous injections of 1-3 U of insulin per rat, doses which would be expected to activate insulin receptors more fully.
(6) Concomitantly, an increase of inositol phosphates and diacylglycerol (DAG) production was observed.
(7) The accumulation of [14C]C20:4-DAG (lower in ET than in saline-infused rats) was paralleled by a decrease in phosphatidylinositol (PI) labelling, whereas phosphatidic acid showed a transient increase by 5 min in saline- but not in ET-infused rats.
(8) To determine the role of the DAG produced upon bombesin stimulation, we examined the effects of another activator of protein kinase C, the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA).
(9) It is concluded that carbachol increases [Ca2+]i by facilitating Ca2+ entry through L-type Ca2+ channels via a 1,2-diacylglycerol (DAG)-protein kinase C (PKC)-dependent pathway while quisqualate mobilizes Ca2+ from inositol 1,4,5-trisphosphate (IP3)-sensitive stores.
(10) In order to characterize the properties of the glyceroglycolipid membranes, ESR spectroscopic studies were carried out with an acyl spin-labeled galactosyl ceramide (SL-GC) or a headgroup spin-labeled phospholipid (SL-6-DPPA) in 1,2-dipalmitoyl[beta-cellobiosyl-(1'---3)]glycerol (Cel-DAG) liposomal membranes.
(11) The E. coli dgkA locus which contains the coding sequences for DAG kinase was subcloned into an eukaryotic expression vector, pMT2.
(12) The mean residence time for pharmacologically active molecules in the body was six times shorter for DAG (1.9 hr) than for DBD (11.4 hr).
(13) These data suggest that PKC is a mediator in the generation of DAG.
(14) These results suggest that DAG accumulation is involved in the potentiating effect of A23187 on CCh-stimulated amylase secretion.
(15) It is concluded that insulin-dependent PDH activation, PIG hydrolysis, and IG and DAG generation are mediated by the wild-type but not by the mutated insulin receptor of Val996.
(16) Since most alloantibodies detected only by 2SP-IAT or LISS-DAG were of doubtful clinical significance, and these techniques produced a high number of unwanted positive reactivities, we conclude that 2SP-IAT and LISS-DAG are not appropriate for the pretransfusion screening for unexpected antibodies.
(17) 261, 8597-8600), lipase from Rhizopus arrhizus selectively degraded the 1-acyl-containing species (DAG), but the ether lipid (EAG) was resistant and was identified and quantified after thin layer chromatography separation.
(18) 1-Oleoyl-2-acetylglycerol, a synthetic DAG analog, stimulated endothelial cell DNA synthesis in a concentration-dependent manner.
(19) Whereas DPG and DS are stable at physiological pH, DAG is unstable, undergoing hydrolysis (regeneration of DF) and rearrangement (intramolecular acyl migration to the 2-, 3- and 4-O-acyl-positional isomers).
(20) Addition of PRL to hepatocyte cultures significantly increased [3H]-glycerol incorporation into DAG within 5 minutes which was followed by a loss of cytosolic PKC activity by 10 minutes.
Sagging
Definition:
(p. pr. & vb. n.) of Sag
(n.) A bending or sinking between the ends of a thing, in consequence of its own, or an imposed, weight; an arching downward in the middle, as of a ship after straining. Cf. Hogging.
Example Sentences:
(1) When the posterior capsule was sectioned, no significant changes were noted in the severity of the sag or the rotation.
(2) Axonal regeneration with the ANG was equal to SAGs as measured by axonal diameters, physiological, and functional methods, although the SAG demonstrated statistically higher axonal counts.
(3) Yards away from a genuine station, he used a huge funnel to fill up a car sagging under the weight of its occupants and market produce.
(4) Guanacline, but not guanethidine or SAG, produced fluorescent lipopigment in all species examined.
(5) For cross-linked alpha alpha, however, the curve sags at temperatures somewhat below the region of principal cooperative loss of helix, the latter occurring at higher temperature but with the same steepness as in the non-cross-linked case.
(6) Myosin ATPase staining showed that about 80% of the LGM consists of type II A fibres, whilst the remainder are type II B. Physiological determination of the contractile properties of motor units indicated two classes of units: those that were relatively fatigue resistant and did not show a sag property (like fast-twitch, fatigue-resistant fibres or FR) and those that were relatively fatigable and did show a sag property (like fast-twitch, fatigable fibres or FF).
(7) The time-dependent sag elicited by hyperpolarization was reduced when Na+ or K+ was removed from the normal bath solution but was abolished with the removal of both Na+ and K+.
(8) In his bid to revitalise Spain's sagging monarchy, Felipe VI must be willing to show that he will handle things differently to his father, said Urreiztieta.
(9) By lacking possibilities of the comparison of equivalent (produced under the same preparation and storage conditions) concentrates of erythrocytes the higher transfusion efficiency of the SAG-S concentrates of erythrocytes to be expected could not be verified.
(10) This domed white building is now a magnet for national expectations, and many wonder whether it will sag under the weight of so much anticipation.
(11) Superantigens (SAg) interact with T lymphocytes bearing particular V beta sequences as part of their T cell receptor (TcR).
(12) Both produce substantial labeling of PC but [14C]SMG gives rise to the highest proportion of TG and the lowest of PA and PI, whereas [14C]SAG yields the opposite pattern.
(13) There is nowhere to go except further into an area of the city 750 metres wide by 500 metres deep that runs along the coast from the television station – with its pair of wrecked and punctured dishes – to the edge of District Two, overlooked by the pavilion and its sagging roof.
(14) As the temperature of the tarts increases a race will start between the sag of melting fat and the drying of the structure-forming gluten network.
(15) Several species were treated chronically with varying doses of guanethidine, guanacline or SAG; the superior cervical ganglia were examined light microscopically for neuronal destruction and for osmiophilic fluorescent lipopigment accumulation.
(16) An important exception concerned SE to which an equal antibody response is produced in high and low lines of sAg selection.
(17) Cheerful and eager to be helpful, he arrives to collect me the following morning, dressed in sagging brown corduroy jacket, faded blue T-shirt, blue silk cravat and socks beneath his Velcro-strapped sandals.
(18) A statistically significant improvement was observed, on the SAG score obtained during the follow-up, in patients (n = 16) who were not admitted to hospital in the 12 month period following discharge.
(19) Jannetta has summarized this concept as follows: "As we age, our arteries elongate and our brains 'sag'.
(20) The observation that ingrowth of SAG neurites to presumptive sensory areas of the inner ear preceded cytodifferentiation of those receptor cells suggested a causal relationship.