What's the difference between decantation and supernatant?

Decantation


Definition:

  • (n.) The act of pouring off a clear liquor gently from its lees or sediment, or from one vessel into another.

Example Sentences:

  • (1) A survey instrument was mailed to a stratified random sample of 1000 nurses from the membership list of the American Association of Critical-Care Nurses to determine whether there are generally accepted standards for decanting intravenous (IV) solutions before the addition of medication.
  • (2) In cultivation of lymphoid cells of the same animals with a nonspecific antigen (tuberculin) the decanted fluids produced no significant cytotoxic action on mous fibroblasts.
  • (3) Water is decanted by opening a faucet connected to the inferior part of the recipient.
  • (4) At the end of the 30-min preincubation period, a 0.2-ml sample was taken for the determination of renin release, and the remaining medium was decanted.
  • (5) We suggest that swabs should not be dipped repeatedly into the flask of liquid nitrogen but that, instead, a small aliquot of nitrogen should be decanted into a smaller 'clean' vessel and a new cotton swab used for each patient.
  • (6) The company, which now intends to move more upmarket, said the crash from profits a year earlier of £112.1 million was largely caused by the impact of recession, but a particularly poor performance from its 250-strong Ratners chain resulted from “adverse publicity” following Gerald Ratner’s infamous description of a decanter set sold by the group as “total crap”.
  • (7) 74 New Church Street, +27 21 423 4530, backpackers.co.za Dutch Manor Facebook Twitter Pinterest This self-styled “antique hotel” is furnished with four-poster beds, leather armchairs, period paintings and porcelain, plus a crystal decanter of sherry for the welcome drink.
  • (8) After incubation, bound and free thyroxine are separated by aspirating or decanting the disc and buffer from the tube.
  • (9) After extraction, the enzyme is heat inactivated for two minutes at 100 degrees C. At this point, the assay can be stopped for 24-48 hours by storage of extraction samples at 2-3 degrees C. The assay is concluded with assembly of standard curve tubes and by addition of antibody, antigens system to all tubes for the final two hour incubation followed by the Dextran charcoal separation of unbound components and the decanting of bound complexes into scintillation counting vials.
  • (10) Lord Carlile, who sits as a non-aligned peer in the House of Lords, told the Observer that the security implications and costs of “decanting” all MPs, peers and palace staff to other buildings around Whitehall should make the authorities think again about the wisdom of such a move.
  • (11) Minelli offers dry cinnamon-and-nutmeg biscuits and an unusual Chinese tea – white monkey paw – which he has meticulously prepared, sticking a thermometer into the kettle, heating the water to precisely 70C, setting a digital alarm for five minutes to allow the tea to brew before decanting it into a vacuum flask.
  • (12) (2) I-125 monoiodoinsulin was used to prevent artifacts resulting from variability in ligand binding due to excessive iodination, (3) separation of free and bound insulin was accomplished by rapid precipitation of hormone-antibody complex with polyethylene glycol, and (4) decanting the supernatants and counting the pellets in the automatic gamma counter.
  • (13) Miller said the new rules were designed to protect "small-scale bloggers" and to "ensure that the publishers of special interest, hobby and trade titles such as the Angling Times and the wine magazine Decanter are not caught in the regime", but Hello!
  • (14) After 5 min centrifugation at 85 g, using an angle head and decantation into a polystyrol tube, second centrifugation.
  • (15) Nonadherent PBL were then removed, after gentle agitation, by decanting and gently washing the monolayer.
  • (16) The consumption of alcoholic beverages stored in lead crystal decanters is judged to pose a hazard.
  • (17) A 315-day feeding trial (F 3) was carried out with fattening bulls (starting weight: approximately 125 kg per animal) during which 4 groups of 7 bulls each were fed 4 mixtures of pelleted food : (1) concentrates (2) concentrates + 50% straw meal (3) concentrates + 25% straw meal and 25% decanted solids from pig faeces (4) concentrates + 50% decanted solids from pig faeces.
  • (18) After exposures ranging from 0 to 60 min, the medium was decanted and cells were harvested.
  • (19) A spokesperson for Newham council said: “We are pleased that we have been able to reach a peaceful, legally binding agreement which allows us to take back the property by 7 October, particularly given the increasingly aggressive nature of the protest.” In a campaign that some have come to see as embodying the capital’s housing crisis in miniature, the women are calling for the estate to be repopulated with those in housing need, for the “decanting” of existing tenants to stop immediately and for demolition to end.
  • (20) Under a schedule accompanying the crime and courts bill, certain magazines will be exempt and will not have to join the new regulator, including hobby magazines, such as Angling Times and Decanter, and scientific journals and community or student publications.

Supernatant


Definition:

  • (a.) Swimming above; floating on the surface; as, oil supernatant on water.
  • (n.) The liquid remaining after solids suspended in the liquid have been sedimented by gravity or by centrifugation. Contrasted with the solid sediment, or (in centrifugation) the pellet.

Example Sentences:

  • (1) After stimulation with lipopolysaccharide and calcium ionophore A23187, culture supernatants of clones c18A and c29A showed cytotoxic activity against human melanoma A375 Met-Mix and other cell lines which were resistant to the tumor necrosis factor, lymphotoxin and interleukin 1.
  • (2) Stimulation is also observed with mixtures of APC expressing DPw3 and APC expressing A1, and likewise, DPw3+ APC become stimulatory when preincubated with supernatants from A1-positive cells.
  • (3) Gel filtration of the 40,000 rpm supernatant fraction of a homogenate of rat cerebral cortex on a Sepharose 6B column yielded two fractions: fraction II with the "Ca(2+) plus Mg(2+)-dependent" phosphodiesterase activity and fraction III containing its modulator.
  • (4) Control incubations revealed an inherent difference between the two substrates; gram-positive supernatants consistently contained 5% radioactivity, whereas even at 0 h, those from the gram-negative mutant released 22%.
  • (5) In fact, the addition of conditioned medium obtained by 48 hr preincubation of isolated monocytes with 10% PF-382 supernatant (M-CM2) or the concomitant addition of supernatant from PF-382 cells (PF-382-CM) and from unstimulated monocytes (M-CM1) are capable of fully replacing the presence of monocytes in the BFU-E assay.
  • (6) Stable factor-dependent B-cell hybridomas were used to monitor the purification of the growth factor from the supernatant of a clonotypically stimulated mouse helper T-cell clone.
  • (7) By contrast, the concentrations of IgA1 kappa and IgA1 lambda in PBMC culture supernatants, both spontaneous and PWM-stimulated, were identical in patients and controls.
  • (8) The 105 000 X g supernatant of the reaction mixture, which contained more than 85% of the thiobarbituric acid-reactive substances, did not inactivate the plasmid DNA.
  • (9) Insulin incubation of plasma membranes pretreated with protease inhibitors (leupeptin, phenylmethylsulfonylfluoride) or with exogenous trypsin, but not chymotrypsin substrates (esters of arginine and tyrosine) yields an inactive supernatant on PDH.
  • (10) Furthermore, exogenous IL-1, IL-2, IL-4, interferon-gamma, or a rat Con A supernatant failed to abrogate DFMO inhibition.
  • (11) Though no strict relationship could be observed between titers in the IH test and the time it took mice to die from the intravenous inoculation of mice (IIM test), results of the supernatants examined by both methods demonstrated that the IH test was more sensitive than the IIM one.
  • (12) The ratio of the metabolically produced Ic to Ib but not the total amount of N-oxygenated metabolites varied greatly depending of the liver microsomal fractions used in the incubation mixtures of Ia; more Ib was produced from Ia using 9000 g supernatant and conversely, more Ic was formed using the washed microsomes of the same liver.
  • (13) In healthy persons the supernatant of lymphocytes preincubated with PHA and ALG was found to show a stimulating effect to clonogenic properties of marrow progenitors, the mentioned effect being not in proportion to the concentration value.
  • (14) The particular advantage of the method described here is the ease with which the supernatants can be collected and transferred to counting vials with minimal handling of radioactive samples.
  • (15) Presence of LAF in the culture supernatants was assessed with the mouse thymocyte assay.
  • (16) Therefore, it appears that although IFN-alpha and IFN-beta are not responsible for the synergizing activity present in activated T cell supernatants, they nonetheless represent a previously unrecognized source of synergizing activity.
  • (17) When spleen cells were further cultured in vitro for 5 days in the presence of Concanavalin-A stimulated rat spleen cell supernatant (Con-A factor), CD4 or CD8 positive cells were detected in the VV (IL-6) injected group, while few positive cells were detected in the control groups.
  • (18) Strong protection was observed in animals vaccinated with 10 ml-equivalent doses of soluble, B. bovis exoantigen-containing supernatant fluids.
  • (19) Fractionation of the supernatant from ventral or dorsal hypothalamic homogenates resulted in a bimodal distribution of IR-TSH.
  • (20) The in vitro metabolism of strychnine was studied in the 9000g supernatant fractions from rat and rabbit livers.

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