What's the difference between diamine and thionine?
Diamine
Definition:
(n.) A compound containing two amido groups united with one or more basic or positive radicals, -- as contrasted with a diamide.
Example Sentences:
(1) The behaviour of DAO suggests that the enzyme plays an important role in the control of intracellular diamine concentration.
(2) The preservation of diamine oxidase activity during starvation implies a need for the enzyme not related to mucosal proliferation or digestion.
(3) Ornithine decarboxylase (ODC) and diamine oxidase (DAO), enzymes involved in polyamine metabolism, are highly expressed by small bowel enterocytes.
(4) Its penetration into the cells was blocked with the diamines putrescine and cadaverine.
(5) 195, 164 168] concluded that low concentrations of diamines reversed the direction of the proton pump.
(6) When administered in combination with the diamine oxidase inhibitor, aminoguanidine, an almost complete block of histamine metabolism was observed.
(7) In bis-(dichloroacethyl)-octamethylen-diamine (bis-diamine)-induced malformed hearts, localization of HNK-1 immunoreactivity was not remarkably altered in the early embryonic heart.
(8) Free polyamines as well as activity of diamine oxidase were distinctly increased in blood, while concentration of total diamino acids was simultaneously decreased in blood serum of the patients as compared with that of healthy persons.
(9) The substitution of an amino group for methyl one in a diamine increased its inhibitory effect.
(10) The authors suggest a method for simultaneous measurements of the blood serum monoamine oxidase and diamine oxidase activities that permits cut down the time of the blood and substrate incubation to 10 min, essentially shortens the procedure and simplifies it.
(11) Forty-one specimens of mucinous cystadenoma (MCA) of the ovary and 13 specimens of normal uterine endocervix were investigated histochemically using alcian blue pH 2.5-PAS, high iron diamine-alcian blue pH 2.5, galactose oxidase-Schiff reaction (GOS, for detection of gastric surface mucous cell mucins), paradoxical Concanavalin A staining (PCS, for detection of gastric gland cell mucins) and periodic acid-sodium borohydride-potassium hydroxide-PAS (PA-SB-PH-PAS) reaction (for detection of mucins of the large intestine).
(12) Sections were treated with nitrous acid and then stained with Alcian Blue pH 1.0, high iron diamine or Aldehyde Fuchsin for sulphated glycoconjugates.
(13) To enhance the efficiency and specificity of diamine methods in light microscopy, these methods were sensitized by silver enhancement in combination with trichloro(ethylene) platinate (KTP).
(14) Inhibition of MDA production to below control levels using the antioxidant N,N'-diphenyl-phenylene diamine (DPPD) however, had no inhibitory effect on cocaine-induced cytotoxicity in either mouse or rat cells.
(15) From all mammals investigated so far only in rabbits diamine oxidase could not be detected in any tissue except the gut.
(16) The objective of the present investigation was to examine the effect of (2R,5R)-6-heptyne-2,5-diamine (methyl-acetylenicputrescine; MAP), an irreversible inhibitor of ODC, on the induction of alloreactivity in vivo.
(17) During early pregnancy the rise of serum diamine oxidase (DAO) is confined within narrow limits to certain weeks of gestation.
(18) Derivatization of carboxyl groups on the carbon surface with a poly(oxyalkalene)diamine (Jeffamine ED-600), followed by biotinylation of the free amine, allowed the attachment of a fluorescein isothiocyanate (FITC) conjugate of ExtrAvidin.
(19) No changes in plasma diamine oxidase activity were observed in saline-treated animals during intestinal ischemia and following revascularization.
(20) Diamine oxidase was also assayed in spent culture media to assess its spontaneous release by CaCo-2 cells.
Thionine
Definition:
(n.) An artificial red or violet dyestuff consisting of a complex sulphur derivative of certain aromatic diamines, and obtained as a dark crystalline powder; -- called also phenylene violet.
Example Sentences:
(1) CIN III nuclei in CS and PEC specimen were Thionin-Feulgen stained and digitized.
(2) It involves the selective staining by the thiazine dye thionine and the interpretations were facilitated by a preceding primary alkylamine O-deacylation step.
(3) On optical analysis, the thiazin dye molecules (azure B, AZURE C and Thionin) are bound radially on the membrane.
(4) The author describes the morphology and distribution of the neurosecretory cells in the supraoesophageal ganglion of the adult female Culex pipiens molestus, using paraldehyde fuchsin and paraldehyde thionine-paraldehyde fuchsin as vital staining techniques.
(5) In attempts to evaluate immunocytochemically autopsy and biopsy material previously obtained and processed for conventional histologic staining, we had to resort to immunostaining of tissues embedded years ago or even sections already stained with hematoxylin-eosin or aldehyde thionin-PAS-orange G. Hypophysial growth hormone and prolactin proved remarkably resistant to such prior treatment with regard to their antigenic properties, and could be readily immunostained in tissue embedded in paraffin 3-4 years earlier, and after destaining of sections prepared up to 7 years earlier.
(6) frequently causes damage to sections and gives inconsistent results because of insufficient primary oxidation and difficulties in making the thionin-Schiff reagent.
(7) In mature light-adapted barley plants, mRNA encoding leaf-specific thionins may reaccumulate if these plants are exposed to pathogens or other stresses.
(8) The enzyme is also sensitive to externally added thionins when expressed in the cytoplasmic compartment of tobacco protoplasts transformed with the Gus gene under the 35S promoter of the cauliflower mosaic virus.
(9) Thionin concentration in mature endosperm of barley cv.
(10) Alcoholic thionin gave the most intense nuclear stain, with a very high reproducibility of the staining pattern.
(11) These values compare to an apparent Km for P. thionin of 1.6 microM for erythrocyte hemolysis and a binding constant of 2.1 microM (Osorio e Castro, V. R. Van Kuiken, B.
(12) The development of the optic tectum and the establishment of retinotectal projections were investigated in the quail embryo from day E2 to hatching day (E16) with Cresyl violet-thionine, silver staining and anterograde axonal tracing methods.
(13) Serial sections were examined by the following procedures: 1) Digestive PAS reaction, 2) High iron diamine-Alcian blue pH 2.5 staining, 3) Modification PAS and Thionin Schiff reaction to differentiate sialic acid, 4) Immuno-peroxidase method.
(14) Parallel to the decline in mRNA content, the de novo synthesis of leaf-specific thionins ceases rapidly upon illumination of etiolated seedlings.
(15) The average cross-sectional area of the total neuronal population as measured in adjacent thionin-stained sections is about 280 micron2.
(16) The rostral-caudal extent of injection sites were mapped in the horizontal plane from sequential coronal, thionin-stained sections and "primary" and "secondary" injection zones were defined according to specific criteria.
(17) A cDNA library, prepared from developing barley endosperm, was screened for thionin recombinants.
(18) The action of the delipidization step and the type of differentiation fluid was assessed for two stains, Harris' hematoxylin and thionin, in frozen sections of rat brains.
(19) The injection sites were histologically confirmed using conventional Thionin stains.
(20) Two hundred and six unselected liver biopsies were stained with Gomori's aldehyde fuschin, aldehyde thionine, and a modified orcein stain, according to Shikata and others (1974).