What's the difference between dilute and fluorescein?

Dilute


Definition:

  • (v. t.) To make thinner or more liquid by admixture with something; to thin and dissolve by mixing.
  • (v. t.) To diminish the strength, flavor, color, etc., of, by mixing; to reduce, especially by the addition of water; to temper; to attenuate; to weaken.
  • (v. i.) To become attenuated, thin, or weak; as, it dilutes easily.
  • (a.) Diluted; thin; weak.

Example Sentences:

  • (1) It includes preincubation of diluted plasma with ellagic acid and phospholipids and a starting reagent that contains calcium and a chromogenic peptide substrate for thrombin, Tos-Gly-Pro-Arg-pNA.
  • (2) Dilutional studies comparing the mechanism of inhibition of monoamine oxidase produced by Gerovital H3 and by ipronizid demonstrated that Gerovital H3 was a reversible inhibitor of monoamine oxidase.
  • (3) A one point dilution enzyme-linked immunosorbent assay (ELISA) procedure suitable for determining immunoglobulin G (IgG) antibody levels to Toxoplasma gondii (T. gondii) in community seroepidemiological surveys is described.
  • (4) Under standardized conditions, the relationship between antigen content and inhibition of chromium release was linear in a semilogarithmic plot, indicating that the antigen content can be determined from testing two dilutions of a given preparation.
  • (5) Rats were injected subcutaneously with 10 ml of air into the dorsal skin to make an air-pouch and with 2 ml of antiserum at an appropriate dilution for passive sensitization, and then 5 ml of air was removed.
  • (6) When the two most toxic isolates (diets) were diluted, survival time increased but severe growth suppression was evident.
  • (7) The immobilizing activity of human normal sera occurred in low titres only, rarely in dilutions of greater than 1:32.
  • (8) The binding follows the principle of isotope dilution in the physiologic range of vitamin B12 present in human serum.
  • (9) Additionally, lymph node cells were cultured under limiting dilution conditions, and the resultant clones here tested for cytotoxicity in the presence or absence of antibodies against Ly2 and LFA-1.
  • (10) A lesser inhibitory effect (a decrease in the rate of precipitation) was observed when gallbladder bile was diluted but was lost after 10-fold dilution.
  • (11) To determine if computed tomography (CT) can accurately measure lung volume, we compared lung gas volume measured by helium dilution with the equivalent volume calculated from CT total lung volume and density in 13 supine dogs.
  • (12) After dilution as above, lipid oxide (LP) significantly increased in III- and II-LDL media, as compared to I- and IV-LDL media.
  • (13) Changes of circulating blood volume during isoflurane or sevoflurane anesthesia were investigated by the dual indicator dilution method in eighteen mongrel dogs.
  • (14) The translocation of nascent PtdSer to the mitochondria was unaffected by 45-fold dilution of the standard reaction thus indicating that the translocation intermediate was unlikely to be a freely diffusible complex.
  • (15) In order to obtain baseline information about Lewis antigen expression in human urothelium in order that changes during malignant transformation can be evaluated, urothelium from eight individuals of known erythrocyte Lewis types were stained by a Tween-modified indirect immunoperoxidase staining technique using goat antibodies directed toward the Lewis a and Lewis b determinants and mouse monoclonal antibodies directed toward the Lewis a determinant in serial dilutions.
  • (16) The diet dilution technique overcomes the major disadvantage of the graded supplementation method for determining the requirements of amino acids, namely that of the amino acid balance changing systematically in successive dietary treatments.
  • (17) The present work describes two methods, the method of instantaneous dilution and that of gel filtration.
  • (18) The best yields occurred in a chemostat at the pH range of 3.5 to 4.5 and temperature of 30 C. A beneficial effect on Ys was observed when the dilution rate (D) was increased.
  • (19) These diets were: diet C consisting of commercial Rat Chow: diet CG, the same diet diluted with 70% glucose calories, diet A, a simulated "American" diet made up of 25 widely used foods, diet AS, the same diet supplemented with small amounts of 25 vitamins and minerals.
  • (20) Three patients reacted with a wheal size greater than or equal to a histamine control at a dilution of 1:1,000 and 3 patients at 1:100.

Fluorescein


Definition:

  • (n.) A yellowish red, crystalline substance, C20H12O5, produced by heating together phthalic anhydride and resorcin; -- so called, from the very brilliant yellowish green fluorescence of its alkaline solutions. It has acid properties, and its salts of the alkalies are known to the trade under the name of uranin.

Example Sentences:

  • (1) We have evaluated the life-span of B lymphocytes by measuring the functional reactivity of normal B cells upon transfer into xid mice, which do not respond to anti-mu, fluoresceinated-Ficoll (FL-Ficoll) and 2,4,6-trinitrophenyl aminoethylcarbamylmethyl Ficoll (TNP-Ficoll).
  • (2) In 60 rhesus monkeys with experimental renovascular malignant arterial hypertension (25 one-kidney and 35 two-kidney model animals), we studied the so-called 'hard exudates' or white retinal deposits in detail (by ophthalmoscopy, and stereoscopic color fundus photography and fluorescein fundus angiography, on long-term follow-up).
  • (3) Fluorescein isothiocyanate (FITC)-ricin exhibited binding to schistosomula and adult worms, but not to cercariae or to freshly transformed schistosomula.
  • (4) The greater biliary excretion and hepatic extraction of cholyl-lys-F relative to free fluorescein further suggest that conjugation with a bile salt may be an efficient way of targeting compounds to the liver.
  • (5) Fluorescein-coupled peanut agglutinin (PNA) has been used at the single-cell level to study mouse lymphocyte subpopulations.
  • (6) Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, biochemical, and serological procedures.
  • (7) Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA.
  • (8) The DNA and protein contents of isolated basal cells were stained with propidium iodide and fluorescein isothiocyanate, respectively, and analysed by flow cytometry using the total protein fluorescence as an estimate of cell size and the DNA fluorescence pulse width as an estimate of nuclear size.
  • (9) Fluorescein-labelled gamma globulin fractions of anti-avidin and anti-uteroglobin both bound to blastocysts, but pure FITC-IgG showed binding only of anti-uteroglobin.
  • (10) The differences between periodate- and NAGO-induced aldehydes were demonstrated by flow cytometry of cells stained with a novel fluoresceinated hydrazide and by an electrophoretic procedure performed with biocytin hydrazide and 125I-streptavidin.
  • (11) For this, rabbit anti-chicken globulin fluorescein isothiocyanate conjugate was used.
  • (12) Active transport of fluorescein across the blood-retinal barrier in the direction of vitreous to blood does not seem to be significant within the first 2 hr after fluorescein injection.
  • (13) The treatment was almost only in those angiopathies successful, in which the fluorescein angiography showed a preponderance of the hyperpermeability over the obliterating process of retinal capillaries.
  • (14) Survivability was judged by morphological appearance, trypan blue exclusion and fluorescein diacetate staining.
  • (15) Cytofluorographic analyses confirmed the increased expression of high-affinity FcR, binding fluoresceinating murine IgG2a.
  • (16) Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry.
  • (17) Indirect immunofluorescence microscopy with fluorescein isothiocyanate-anti-mouse immunoglobulin G also identified the antigen on the surface of cultured and fresh tumor cells and detected the antigen on 16 of 17 Grade 3 bladder tumor specimens as well as on some kidney and testicular tumor cells.
  • (18) The expression of EBVR was established by the binding of fluorescein isothiocyanate (FITC)-labeled EBV to the cells, while surface markers were stained by phycoerythrin (PE)-indirect immunofluorescence, using monoclonal antibodies.
  • (19) IC50 values of 6.8.10(-9), 1.7.10(-8), 1.8.10(-8) and 5.4.10(-9) M were measured in rat liver membranes for NBD-, fluorescein-, rhodamine-Trp25-glucagon and native glucagon, respectively.
  • (20) Sodium fluorescein and fluorescinated dextrans (FD) of selected molecular weights were combined and perfused into the anterior chamber of normal and inflamed eyes of cynomolgus monkeys.