(n.) A small point or spot, made with a pen or other pointed instrument; a speck, or small mark.
(n.) Anything small and like a speck comparatively; a small portion or specimen; as, a dot of a child.
(v. t.) To mark with dots or small spots; as, to dot a line.
(v. t.) To mark or diversify with small detached objects; as, a landscape dotted with cottages.
(v. i.) To make dots or specks.
Example Sentences:
(1) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
(2) Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression.
(3) The method described uses film DOT-I and DOT-II by Dupont, whereby the exposure of the step wedge takes place on a linear accelerator with a photo energy of 10 MeV.
(4) The results indicated a very good comparability between the dot-blot assay and IF-tests, and this dot-blot method was ascertained as a simple and useful method for the scrub typhus serodiagnosis.
(5) Using a silver staining technique (AgNOR technique), we have investigated the nucleolar organizer-associated proteins (NORs) in formalin-fixed paraffin embedded conjunctival specimens of 15 intraepithelial squamous carcinomas, 10 hyperplastic-dysplastic samples and 10 control epithelial fragments; the mean number of intranuclear black dots was determined for each case.
(6) An increase in cytoplasmic PRL mRNA content was evident in all the animals treated with estrogen as revealed with cytoplasmic dot blot analysis.
(7) All these strains produced an enterotoxic principle, antigenically related to cholera coli family of enterotoxins, as detected by latex agglutination and immuno-dot-blot tests.
(8) ELISA, cDNA dot blot hybridization and transmission by vector aphids were used to investigate the occurrence and degree of cross-protection produced in oat plants by virus isolates representing five strains or serotypes of barley yellow dwarf virus, namely PAV, MAV, SGV, RPV and RMV.
(9) With anti-Go alpha antibodies, the immunofluorescence was more clearly focussed on a dotted pattern and the co-location with the voltage-dependent Ca2+ channel immunoreactivity indicates that both proteins were located in very close subcellular structures.
(10) Thresholds were measured for detecting perturbations in a regular lattice of dots by modulating local dot density, local dot luminance, or some combination of the two.
(11) As revealed by a dot immunobinding assay, the amount of immunoreactive bovine and human MFGM-associated antigen increased dramatically with the onset of lactation after delivery.
(12) All nine responders were negative for HBV DNA in serum by dot blot during or after treatment, but seven remained positive by polymerase chain amplification and Southern-blot hybridization.
(13) DNA sequencing and oligonucleotide dot-blot analysis of class II genes from two race-specific haplotypes indicate that susceptibility to IDDM is closely linked to the DQA1 locus and suggest that both the DQB1 (ref.
(14) The number of dots, the summed area of dots and the MF were separately evaluated by the "t" test.
(15) Hepatitis B virus DNA (HBV-DNA) in serum was studied in 67 anti-HBe patients using dot-blot hybridization, a modified technique and polymerase chain reaction (PCR).
(16) Dot Masr reporter Mohamed Abu Asay says in the past he could never report on the budget deficit in Egypt and the Saudi-Egyptian military operation in Yemen in the same piece.
(17) Nuclei from the normal mouse liver were partially digested with micrococcal nuclease, followed by DNA extraction, agarose gel electrophoresis and dot blot hybridization with 32P-labeled cDNA probes of CPS1 and ACT complex.
(18) Reconstituted flagellar filaments were demonstrated by three complementary methods: transmission electron microscopy, antigenic reactivity with H7 antiserum by a dot blot immunoassay, and immunogold localization of antiserum raised to the purified antigen to intact flagella on whole E. coli O157:H7.
(19) Rather, the sequences of alpha-amylase mRNA are rapidly degraded during heat shock as shown by in vitro translation and dot blot hybridization with a cDNA probe.
(20) Prism fixation disparity curves were determined in three different experimental situations: the routine method according to Ogle, a method to stimulate the synkinetic convergence (Experiment I, with one fixation point as sole binocular stimulus) and a method to stimulate the fusion mechanism (Experiment II, with random dot stereograms).
Speckled
Definition:
(imp. & p. p.) of Speckle
(a.) Marked or variegated with small spots of a different color from that of the rest of the surface.
Example Sentences:
(1) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
(2) Ensemble averaging of a large number of unfiltered spectra was used as the "gold standard" in the evaluation, i.e., as the output of an ideal filter which reveals the exact nature of the underlying Doppler spectrum after speckle has been eliminated.
(3) Rather than the predominant homogeneous pattern seen elsewhere in systemic lupus erythematosus and rheumatoid arthritis, the speckled pattern was commonest among Thai patients with these two diseases (67.9% and 76.9% respectively).
(4) The localization of these key components of the pre-mRNA splicing machinery to speckled nuclear regions suggests that these regions may be involved in pre-mRNA splicing.
(5) The commonest FANA pattern is the speckled pattern.
(6) It is shown that there exists a trade-off between speckle reduction and reproduced density range.
(7) The secretory granules of the pro-acinar cells contained speckled or rod-like substructures which stained intensively for carbohydrates and were digested by proteolytic enzymes.
(8) Clinical and laboratory findings were correlated from 46 patients with IgG localization in epidermal nuclei in a speckled (particulate) pattern on direct immunofluorescence of normal skin.
(9) A 2 year-old girl presented with bacterial meningitis followed by a lupus erythematosus syndrome consisting of erythematous rash, Raynaud's phenomenon and mutilating cicatricia atrophy; high speckled antinuclear antibodies and anti-Sm and anti-Ro antibodies titers; a selective absence of C4; moderate mesangial proliferation with IgM and C1q mesangial deposits.
(10) Indirect immunofluorescence examination of cultured human embryo cells, using the serum of patients with nasopharyngeal cancer, showed a speckled nuclear pattern.
(11) A new online signal processing technique is described to reduce speckle noise in ultrasound images.
(12) Serological examination revealed antinuclear antibodies with a titer of 1:5120 (speckled pattern) and anti-RNP antibody with a titer of 1:32.
(13) Time-average speckle interferometry has been applied to obtain displacement patterns on the chest wall produced by cardiac action, in the absence of breathing, during various phases of the cardiac cycle.
(14) We present applications to speckle reduction, detection of specular reflectors, attenuation estimation and ultrasound imaging.
(15) Live animal speckle scores classified carcasses as Select or Choice with 77% accuracy.
(16) A unique sparsely speckled antinuclear antibody pattern was seen.
(17) The sand was brown-red and the speckles of salt sparkled in the sun.
(18) They re-enter the newly formed nuclei of the two daughter cells at early telophase, producing speckled nuclear fluorescent patterns typical of interphase cells.
(19) This agrees with earlier manual-visual measurements but the higher precision due to computer evaluation of the speckle patterns gives much more reliable estimate of the repositioning error.
(20) The monoclonal antibody reacted with the 350-kDa protein in immunoblot analysis and immunostained intranuclear speckles; both immunoreactions were abolished by treatment with alkaline or acid phosphatase.