What's the difference between dye and safranine?

Dye


Definition:

  • (v. t.) To stain; to color; to give a new and permanent color to, as by the application of dyestuffs.
  • (n.) Color produced by dyeing.
  • (n.) Material used for dyeing; a dyestuff.
  • (n.) Same as Die, a lot.

Example Sentences:

  • (1) The most successful dyes were phenocyanin TC, gallein, fluorone black, alizarin cyanin BB and alizarin blue S. Celestin blue B with an iron mordant is quite successful if properly handled to prevent gelling of solutions.
  • (2) The actions of the polyvalent cationic dye Ruthenium Red and the enzyme neuraminidase were studied at the frog neuromuscular junction.
  • (3) Significant increases in the extravasation of dye were observed in both animal groups sensitized with IgG1 and IgG2 antibodies.
  • (4) While the reduced form of the "derived" polyphenolic compounds, generated during tissue homogenization, appeared to enhance dye binding with bovine serum albumin, their influence on the protein assay directly in crude homogenates was extremely diverse.
  • (5) To selectively stain polyanionic macromolecules of growth plate cartilage and to prevent artifacts induced by aqueous fixation, proximal tibial growth plates were excised from rats, slam-frozen, and freeze-substituted in 100% methanol containing the cationic dye Alcian blue.
  • (6) This dye is concentrated and secreted by the parietal cells.
  • (7) The rhodamine 123-induced growth inhibition was partially reversed by treating the dye-pre-exposed infected erythrocytes with the proton ionophore carbonyl-cyanide m-chlorophenylhydrazone, which dissipates transmembrane proton gradients.
  • (8) The duration of electrophoresis was based on the migration of a marker dye for a predetermined distance.
  • (9) Effects of fixation with glutaraldehyde (GA), glutaraldehyde-osmium tetroxide (GA-OsO(4)), and osmium tetroxide (OsO(4)) on ion and ATP content, cell volume, vital dye staining, and stability to mechanical and thermal stress were studied in Ehrlich ascites tumor cells (EATC).
  • (10) By using pH- or Ca(2+)-sensitive dyes and recording at the ion-sensitive and -insensitive (isosbestic) wavelengths, the method can measure both cell volume changes and intracellular ionic activities.
  • (11) An argon dye laser system with lambda em=630 nm (400 mW cm-2) was used for PDT with a total light dose of 400 J cm-2.
  • (12) In a complete system, consisting of a dye-donor couple, ferredoxin, thioredoxin and ferredoxin-thioredoxin reductase, light activation of purified spinach fructose-1,6-bisphosphatase was observed in vitro.
  • (13) When given 30 min after acetic acid instillation SC-41930 prevented the rise in myeloperoxidase and dye extravasation observed in the acetic acid inflammed tissue.
  • (14) A comparison was made between the Q's estimated by the CO2 rebreathing method during tethered swimming and previously published data on Q determined by the dye-dilution method during free swimming in a flune.
  • (15) A novel staining procedure for enumerating osteoclasts on neonatal mouse calvaria with the vital fluorescent dye acridine orange is described.
  • (16) Thus, angiographic dye appears to decrease heart rate by a direct effect on pacemaker tissue and by reflex vagal suppression of the sinus pacemaker.
  • (17) The purpose of this study was to compare the level of apical dye penetration when different sealers were used.
  • (18) This protein which we call CBP-58 bears similarities to the endoplasmic reticulum protein, calreticulin, in that it has a pI of 4.7 containing approximately 30% glutamate and aspartate, has a high capacity for calcium, and stains blue with the carbocyanine dye, 'Stains-all'.
  • (19) Minced and triturated fragments from the spinal cord of normal rat fetuses (15-18 days gestation) labeled with the fluorescent dye fast blue (FB) were successfully transplanted into juvenile myelin-deficient rat spinal cord under direct observation.
  • (20) In vitro, the soluble core PEI and membrane both bound reactive substances of limited aqueous stability, such as from [14C]N-methyl-N-nitrosourea ([14C]NMU), and aqueous stable dyes of molecular weight up to 1000.

Safranine


Definition:

  • (n.) An orange-red nitrogenous dyestuff produced artificially by oxidizing certain aniline derivatives, and used in dyeing silk and wool; also, any one of the series of which safranine proper is the type.

Example Sentences:

  • (1) Stage IV cells contain only safranin-positive granules.
  • (2) In 13- and 15-day embryos, mast cells showed a pale metachromasia with toluidine blue, and stained blue with Alcian Blue-Safranin O (AB-S).
  • (3) At 30 and 60 days, an S-100 positive band of cells separated a deep safranin-O positive hypertrophic layer from a fibrocellular surface layer.
  • (4) The increase of cAMP level also affected the maturation of mast cells, as the ratio of cells of mixed granulation increased, compared to the alcian blue- and safranin-present model inhibited degranulation.
  • (5) Astra blue (AB) safranine stained highly significantly more mast cells than did toluidine blue.
  • (6) There was a profound loss of safranin-O stainability in the nucleus pulposus, annulus fibrosus and cartilagenous endplate.
  • (7) Other staining techniques assessed were the modified Ziehl-Neelsen, safranin-methylene blue and auramine-phenol fluorescence.
  • (8) A zone of safranin O depletion was present in the ventral anulus fibrosus adjacent to the nucleus pulposus in all treated discs, indicating proteoglycan loss.
  • (9) The distribution and localization of S-100 protein was compared with safranin-O staining and H and E morphology in relatively unaffected, degenerative, and osteophytic regions of human articular cartilage from 26 joints obtained at the time of total joint replacement for osteoarthritis.
  • (10) Both safranin O and Giemsa were suitable nonfluorescent staining techniques; lomofungin was not.
  • (11) After 8-12 weeks, however, many subserosal mast cells became positive for berberine sulfate and safranin.
  • (12) Defects in treated joints contained Safranin O staining material that was histologically similar to a disorganized hyaline cartilage.
  • (13) Intestinal MC stained with the same dyes as oral MC except for 0.005% toluidine blue, berberine sulfate, and safranin.
  • (14) A new microspectrophotometric method was developed for quantitation of glycosaminoglycans with Safranin O dye in articular cartilage matrix.
  • (15) It is concluded that the use of safranine to monitor the changes in membrane potential during Ca2+ transport by mitochondria should be avoided or special care be taken.
  • (16) The selectivity of myoepithelial cell staining is enhanced by oxidation of sections, nuclear staining by Safranin-O, and differentiation with Tartrazine.
  • (17) Ferric ion-ferrocyanide staining and safranin-0-counterstaining of neocortical tissue from cats with GM1 gangliosidosis have established that pyramidal neuron meganeurites occur proximal to axonal initial segments and that they are distinct from axonal spheroids.
  • (18) Cytochrome oxidase reconstituted vesicles, supplemented with ascorbate and cytochrome c. induce large spectral changes of the positive dye safranine, reversed by uncouplers and inhibitors of respiration.
  • (19) Histologic specimens of the articular cartilage were stained with Safranin-O to assess proteoglycans-enhanced chrondrocyte function, and the synovium was stained with pentachrome.
  • (20) Synaptosomes were severely energy-deprived, because anaerobic glycolysis was inhibited by 90% from the aerobic level and mitochondrial membrane potential dropped below the limit that could be detected by the safranine method.

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