(n.) An unorganized or unformed ferment, in distinction from an organized or living ferment; a soluble, or chemical, ferment. Ptyalin, pepsin, diastase, and rennet are good examples of enzymes.
Example Sentences:
(1) In contrast, DNA polymerase alpha, the enzyme involved in chromosomal DNA replication, was relatively insensitive to CA1.
(2) The accumulation of lipids and enzymes such as simple estarase, lipase, beta-HDH, alpha-GDH and NADPH-reductase in those areas, suggests that lipids are not a simple excretory product.
(3) It was found that the skeletal muscle enzyme of the chick embryo is independent of the presence of creatine and consequently is another constitutive enzyme like the creatine kinase of the early embryonic chick heart.
(4) Previous attempts to purify this enzyme from the liquid endosperm of kernels of Zea mays (sweet corn) were not entirely successful owing to the lability of partially purified preparations during column chromatography.
(5) These results demonstrate that increased availability of galactose, a high-affinity substrate for the enzyme, leads to increased aldose reductase messenger RNA, which suggests a role for aldose reductase in sugar metabolism in the lens.
(6) As far as acrophase table is concerned for all enzymes and fractions the acrophase occurred during the night.
(7) This theory was confirmed by product analysis and by measuring the affinity of the substrate for the enzyme by its inhibition of p-nitrophenyl glucoside hydrolysis.
(8) This result demonstrates that branching enzyme belongs to a family of the amylolytic enzymes.
(9) These results are discussed in relation to the possible existence of enzyme-bound intermediates of nitrogen fixation.
(10) During enzyme purification two nucleases were identified.
(11) Binding data for both ligands to the enzyme yielded nonlinear Scatchard plots that analyze in terms of four negatively cooperative binding sites per enzyme tetramer.
(12) In the measurement, enzyme-labeled and unlabeled antigens (Ag* and Ag) were allowed to compete in binding to the antibody (Ab) under conditions where Ag* much less than Ab much less than Ag.
(13) Nucleotide, which is essential for catalysis, greatly enhances the binding of IpOHA by the reductoisomerase, with NADPH (normally present during the enzyme's rearrangement step, i.e., conversion of a beta-keto acid into an alpha-keto acid, in either the forward or reverse physiological reactions) being more effective than NADP.
(14) The enzyme, when assayed as either a phospholipase A2 or lysophospholipase, exhibited nonlinear kinetics beyond 1-2 min despite low substrate conversion.
(15) The specific limited trypsinolysis of bacteriophage T7 RNA polymerase (T7RP) was performed in the presence of various components of the polymerase reaction and some GTP-analogs--irreversible inhibitors of the enzyme.
(16) Hepatic enzyme elevations were more dramatic after blunt trauma, reflecting greater hepatocellular disruption.
(17) Treatment of the bound F1-ATPase with 4-chloro-7-nitrobenzofurazan prevented complete release of the enzyme by ATP.
(18) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
(19) The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei.
(20) We have measured the antibody specificities to the two polysaccharides in sera from asymptomatic group C meningococcal carriers and vaccinated adults by a new enzyme-linked immunosorbent assay (ELISA) procedure using methylated human serum albumin for coating the group C polysaccharide onto microtiter plates.
Rennin
Definition:
(n.) A milk-clotting enzyme obtained from the true stomach (abomasum) of a suckling calf. Mol. wt. about 31,000. Also called chymosin, rennase, and abomasal enzyme.
Example Sentences:
(1) Whey obtained by acid precipitation or by the application of rennin was devoid of bactericidal activity but was capable of slowing down proliferation of E coli.
(2) Mucor rennin was efficiently excreted from the yeast host as a heavily glycosylated form.
(3) One-day-old rats have a protease with a pH optimum of 3.8 to 4.2 similar to that of calf rennin.
(4) Another expression system for production of Mucor rennin in Saccharomyces cerevisiae was also established.
(5) The greatest differences between experimental and control cheeses (produced with rennin) in the contents of the above-mentioned constituents were observed within the first 30 days.
(6) The lactogenic response of mouse mammary gland explants to human placental lactogen (hPL) and ovine pituitary prolactin (oPRL) was examined on days 10 to 18 of pregnancy by measuring 3H-amino acid incorporation into calcium-rennin precipitable casein.
(7) Activation of the four separate components of prochymosin (prorennin) at pH 5.0 demonstrated that each zymogen was the precursor to an electrophoretically distinct chymosin (rennin).
(8) Polyacrylamide-gel electrophoresis confirmed the chromatographic results, but crystalline rennin was shown to consist of four bands.
(9) This was done using the yeast GAL7 promoter and the prepeptide sequence of a fungal aspartic proteinase, Mucor pusillus rennin (MPR).
(10) The curves were characteristic of a limited, specific attack by rennin on these proteins.
(11) The stomach of newborn pig contains a proteinase that is immunologically closely related to calf chymosin (rennin) (EC 3.4.23.4.).
(12) It is concluded that a I:I mixture of porcine pepsin and rennin may be used for the production of small medium hard cheeses without impairing their quality of nutritive value.
(13) Both milk-clotting proteases have their optimum activity at pH 5.2 and 45 degrees C. The microbiological rennin has a second maximum activity at pH 3.5 and 55 degrees C. Temperatures above 55 degrees C cause a rapid decrease of activity.
(14) The prepro-peptide of fungal aspartic proteinase, Mucor pusillus rennin, is useful as a secretion leader for efficient secretion of human growth hormone (HGH) from Saccharomyces cerevisiae.
(15) Comparative studies have been made on the effects of diazoacetyl-DL-norleucine methyl ester (DAN), 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) and pepstatin on acid proteases, including those from Acrocylindrium sp., Aspergillus niger, Aspergillus saitoi, Mucor pusillus, Paecilomyces varioti, Rhizopus chinensis, and Trametes sanguinea, and also porcine pepsin [EC 3.4.23.1] and calf rennin [EC 3.4.23.4] for comparative purposes.
(16) Simple, reliable procedures for the assay of pepsin and rennin-like enzyme activities are described as a means of identifying gastric fluid-containing samples in forensic science laboratories.
(17) Aspergillus flavus produced extracellularly an active rennin-like enzyme when grown aerobically in whey media.
(18) When anti-sera to bovine pepsinogen and chymosin (rennin) was used, immunoreactive tumor cells were found in 12 of 23 gastric adenocarcinomas irrespective of the tumor subtype, degree of differentiation, or the presence or absence of intestinal metaplasia in the adjacent gastric mucosa.
(19) Calf Chymosin and a fungal protease from Mucor pusillus (Mucor rennin) are members of the aspartic proteinases used as milk-coagulants in cheese industry.
(20) The role of individual amino acid residues in the 98-102 and 111-112 regions of bovine kappa-casein in its interaction with the milk-clotting enzyme chymosin (rennin) was investigated.