What's the difference between esculin and polychrome?
Esculin
Definition:
(n.) A glucoside obtained from the Aesculus hippocastanum, or horse-chestnut, and characterized by its fine blue fluorescent solutions.
Example Sentences:
(1) Although treatment with beta-carotene tended to decrease the incidence and number of large intestinal carcinomas, beta-carotene, selenium, esculin and eugenol all decreased the incidence of kidney nephroblastomas, the differences were not statistically significant.
(2) Methodology for reading and interpreting positive reactions and time of incubation of the bile-esculin medium was defined.
(3) All isolates produced acetoin from glucose and hydrolyzed esculin.
(4) Esculin was hydrolyzed only by the biotype 1, serotype O:6 strain.
(5) These data indicate that the bile-esculin test provided a reliable means of identifying group D streptococci and differentiating them from non-group D streptococci.
(6) We examined 113 strains of fresh clinical isolates of E. coli and assessed the ability of colonies in a population to hydrolyze esculin with and without preincubation in inducible substrates at 24, 48, and 72 h. The number of strains capable of fermenting salicin, a sugar with a beta-glucoside linkage like esculin, was studied under the same conditions.
(7) The non-O1 strains displayed strong enzymatic and hemolytic activities, except for esculin hydrolysis.
(8) Recognition of Listeria colonies is evident by black discoloration of the medium due to esculin hydrolysis without need for special illuminating equipment.
(9) The phenotypic characteristics of group 1 were similar to those of a previously described CDC group designated A-4, with the major differentiating characteristic being the inability to hydrolyze esculin.
(10) We then used the hemolytic reactions, susceptibility of bacitracin and sulfamethoxazole-plus-trimethoprim disks, CAMP disk reactions on sheep blood Trypticase soy agar and bile-esculin and 6.5% NaCl agar tolerance tests with incubation in normal atmosphere.
(11) This asaccharolytic organism does not metabolize esculin, urea, meat, gelatin, casein, or nitrate.
(12) It appeared that the esculin hydrolysis enzyme(s) of E. coli was inducible rather than constitutive.
(13) Four additional tests (esculin hydrolysis, acetoin production, lysine decarboxylation, and gas production from glucose) identified within 24 hr all examples of the three common species of Aeromonas.
(14) Fuchsin glistening colonies as well as the total bacterial counts on Sabouraud agar and Leifson agar as well as on kanamycin-esculin agar showed frequency peaks which were one power of ten lower.
(15) The organism was gram-negative, produced hemolysis on blood agar, and gave a positive reaction for oxidase, urease, o-nitrophenyl-beta-D-galactopyranoside, and esculin.
(16) Twenty-one strains, representing five serotypes, were esculin- and salicin-negative, and were considered to be the primary cause of disease in the majority of these cases.
(17) The organism is catalase- and oxidase-positive, hydrolyzes esculin, and forms acid in glucose, xylose, and maltose after 21 days' incubation.
(18) Seven of these eighteen tests were used to construct a flexible, dichotomous key, Aerokey II, for identifying clinical aerontonads: esculin hydrolysis, gas from glucose, acid from arabinose, indole production, acid from sucrose, Voges-Proskauer reaction, and resistance to cephalothin (30 micrograms).
(19) A set of six diagnostic tests (production of hydrogen peroxide, arginine and esculin hydrolysis, polysaccharide production, Voges-Proskauer reaction, mannitol fermentation) was used for speciation of the viridans streptococci; in addition, search for spreading zones around the colonies was included in the diagnostic procedure.
(20) Of the isolates 100% were positive in the following reactions: catalase production, nitrate and nitrite reduction, esculin hydrolysis, and acid production from glucose, sucrose, maltose, salicin, and glycerol.
Polychrome
Definition:
(n.) Esculin; -- so called in allusion to its fluorescent solutions.
(a.) Executed in the manner of polychromy; as, polychrome printing.
Example Sentences:
(1) These findings are supported by animal experiments in rabbits with the aid of the polychrome sequence marking technique using fluorochromes.
(2) Experiments in sheep, dogs, and rabbits combining intravital staining of blood circulation and polychrome fluorescent labeling of bone remodeling leads to the conclusion that early bone porosis in the vicinity of the implants is the result of internal remodeling of cortical bone and is induced by necrosis rather than by unloading.
(3) The following methods were used: polychrome injection of arteries, veins, lymph capillaries and vessels, macro-microdissection, staining after van Gieson and with hematoxylin-eosin, impregnation with 0,25--1% solution of silver nitrate, dehydration and clearing, calculation of thickness of loops per 1 mm2 and their depth by means of ocular micrometer.
(4) The tannic acid-polychromic stain method (Mason et al., 1985) designed for the identification of the cultured alveolar type II cells has been adapted to cryostat sections of human lungs.
(5) Polychrome sequence marking and histological tests demonstrated that filling up of bony defects started later with turbine-driven drills than with drills of 2,000 r.p.m.
(6) Impressive polychrome wall murals painted on plaster converted Teotihuacan into a painted city.
(7) High-performance liquid chromatographic analysis of degraded stain samples has shown the products of methanolic degradation to be different from those obtained in aqueous polychroming reactions.
(8) Polychrome injection of arteries and veins was made.
(9) Improved polychrome staining of 1-1.5 mum epoxy sections is achieved with sequential applications of a single basic fuchsin-methylene blue mixture at two different pH values.
(10) Useful extensions of CL microscopy to the field of spectral imaging are obtained with the introduction of a slit, a polychromator and an area detector, substituting for the linear imager.
(11) Drăgan's high-speed PBT (polychrome blue tannin) staining method enables cellular elements to be detected in oral smears in one minute at most.
(12) The different classes of immunoglobulins and their kinetics are determined by the use of monospecific antibodies linked to different enzymes which give a polychromic specific staining.
(13) The staining abnormalities of the first degree disorders are characterized by nonreactivity to the Polychrome stain of the Papanicolaou technique as well as to scarlet red stain of the Shorr technique.
(14) The image of the distribution of 81Br- or 19F-, coded in another color scale, can be superimposed, giving a polychromic image of the cell, thus showing the intracellular localization of the compound.
(15) At 60 C polychroming is rapid, the absorption peak reaching 640-620 nm in 3 days.
(16) The TBLN are revealed by means of polychromic injection of Gerota mass into the lung tissue, or directly into the lymph nodes revealed.
(17) The liver tissue specimens were fixed in OsO4, embedded in epoxy resin, cut 4 microns thick and stained with polychrome.
(18) Polychromic injection of arteries, veins and lymphatic bed, staining of preparations after van Gieson, Weigert, with hematoxylin-eosin and morphometry were the methods applied.
(19) We developed a tannic acid and polychrome stain that can visualize these intracellular inclusions in rat alveolar type II cells by light microscopy and that can be used for autoradiography.
(20) These deposits were polychromic, exhibiting blue, green, red, pink, orange, gold, and yellow colors.