What's the difference between expression and primer?

Expression


Definition:

  • (n.) The act of expressing; the act of forcing out by pressure; as, the expression of juices or oils; also, of extorting or eliciting; as, a forcible expression of truth.
  • (n.) The act of declaring or signifying; declaration; utterance; as, an expression of the public will.
  • (n.) Lively or vivid representation of meaning, sentiment, or feeling, etc.; significant and impressive indication, whether by language, appearance, or gesture; that manner or style which gives life and suggestive force to ideas and sentiments; as, he reads with expression; her performance on the piano has expression.
  • (n.) That which is expressed by a countenance, a posture, a work of art, etc.; look, as indicative of thought or feeling.
  • (n.) A form of words in which an idea or sentiment is conveyed; a mode of speech; a phrase; as, a common expression; an odd expression.
  • (n.) The representation of any quantity by its appropriate characters or signs.

Example Sentences:

  • (1) Here we have asked whether protection from blood-borne antigens afforded by the blood-brain barrier is related to the lack of MHC expression.
  • (2) Similar experimental manipulation has yielded in vitro lines established from avian B-cell lymphomas expressing elevated levels of c-myc or v-rel.
  • (3) When micF was cloned into a high-copy-number plasmid it repressed ompF gene expression, whereas when cloned into a low-copy-number plasmid it did not.
  • (4) We also show that proliferation of primary amnion cells is not dependent on a high c-fos expression, suggesting that the function of c-fos is more likely to be associated with other cellular functions in the differentiated amnion cell.
  • (5) Injection of resistant mice with Salmonella typhimurium did not result in the induction of a population of macrophages that expressed I-A continuously.
  • (6) Stimulation is also observed with mixtures of APC expressing DPw3 and APC expressing A1, and likewise, DPw3+ APC become stimulatory when preincubated with supernatants from A1-positive cells.
  • (7) BL6 mouse melanoma cells lack detectable H-2Kb and had low levels of expression of H-2Db Ag.
  • (8) These studies show that metabolic activation is necessary for the expression of the mutagenic activity of aflatoxins B1 and G1 in N. crassa.
  • (9) We also show that the gene of the main capsid protein is expressed from its own promoter in an Escherichia coli strain.
  • (10) Using the oocyte system to express size-fractionated mRNA, we have also determined that the mRNA coding for this protein is between 1.9-2.4 kilobases in length.
  • (11) Because many wnt genes are also expressed in the lung, we have examined whether the wnt family member wnt-2 (irp) plays a role in lung development.
  • (12) A beta-adrenergic receptor cDNA cloned into a eukaryotic expression vector reliably induces high levels of beta-adrenergic receptor expression in 2-12% of COS cell colonies transfected with this plasmid after experimental conditions are optimized.
  • (13) Four other independent LCMV-GP2(275-289) specific H-2Db-restricted CTL clones also expressed V alpha 4 and V beta 10 gene elements.
  • (14) Maximal yields of lipid and aflatoxin were obtained with 30% glucose, whereas mold growth, expressed as dry weight, was maximal when the medium contained 10% glucose.
  • (15) Recent studies have shown that an aberration in platelet-derived growth factor gene expression is unlikely to be a factor in proliferation of smooth-muscle cells.
  • (16) Thus, human bronchial epithelial cells can express the IL-8 gene, with expression in response to the inflammatory mediator TNF regulated mainly at the transcriptional level, and with elements within the 5'-flanking region of the gene that are directly or indirectly modulated by the TNF signal.
  • (17) In concert with TF expressed by monocytes and macrophages this endothelial cell procoagulant activity may play a role in the pathogenesis of thrombotic disease.
  • (18) The possibility that both IL 2 production and IL 2R expression are autonomously activated early in T cell development, before acquisition of the CD3-TcR complex, led us to study the implication of alternative pathways of activation at this ontogenic stage.
  • (19) A domain containing a CA repeat, similar to ones found in other late, cAMP-induced Dictyostelium genes, is required for cAMP-induced and developmental expression.
  • (20) This study examines the role of sex hormones in modulating the expression of autoimmunity in NZB x NZW F1 mice.

Primer


Definition:

  • (n.) One who, or that which, primes
  • (n.) an instrument or device for priming; esp., a cap, tube, or water containing percussion powder or other compound for igniting a charge of gunpowder.
  • (a.) First; original; primary.
  • (n.) Originally, a small prayer book for church service, containing the little office of the Virgin Mary; also, a work of elementary religious instruction.
  • (n.) A small elementary book for teaching children to read; a reading or spelling book for a beginner.
  • (n.) A kind of type, of which there are two species; one, called long primer, intermediate in size between bourgeois and small pica [see Long primer]; the other, called great primer, larger than pica.

Example Sentences:

  • (1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
  • (2) Elongation of existing RNA primers by the human polymerase-primase was semi-processive; following primer binding the DNA polymerase continuously incorporated 20 to 50 nucleotides, then it dissociated from the template DNA.
  • (3) This cDNA was obtained because of an identical 10 bp match with the 3' end of one of the GnRH primers.
  • (4) When PCR products in each of the 12 cats were subjected to a second amplification using the same primer pair (two-step amplification: double PCR), FIV proviral DNA was detected in all of the cats.
  • (5) In junctions, 3' PSS termini are preserved by fill-in DNA synthesis, although their 5' recessed ends cannot serve as a primer.
  • (6) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
  • (7) 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension.
  • (8) Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension.
  • (9) Primer extension and S1 nuclease protection analysis demonstrate that the hepatic and kidney specific mRNAs are transcriptionally initiated at different sites within the sialyltransferase gene.
  • (10) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
  • (11) Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5'-flanking DNA sequences.
  • (12) Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences.
  • (13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
  • (14) This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain.
  • (15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
  • (16) B.subtilis phage M2 uses a protein, instead of RNA, as the primer of its DNA replication.
  • (17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
  • (18) Primer extension experiments show that in fission yeast transcripts are initiated at the same starting point as in tomato, indicating for the first time that a plant promoter can be correctly recognized in fission yeast.
  • (19) Genomic clones for the mouse estrogen receptor have been isolated from a cosmid library and used in conjunction with the cDNA clones to study the expression of the receptor in vivo by RNase mapping, primer extension, and Northern blotting.
  • (20) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.