(n.) The act or process of filtering; the mechanical separation of a liquid from the undissolved particles floating in it.
Example Sentences:
(1) Gel filtration of the 40,000 rpm supernatant fraction of a homogenate of rat cerebral cortex on a Sepharose 6B column yielded two fractions: fraction II with the "Ca(2+) plus Mg(2+)-dependent" phosphodiesterase activity and fraction III containing its modulator.
(2) In this review, we demonstrate that serum creatinine does not provide an adequate estimate of glomerular filtration rate (GFR), and contrary to recent teachings, that the slope of the reciprocal of serum creatinine vs time does not permit an accurate assessment of the rate of progression of renal disease.
(3) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
(4) The authors conclude that H. pylori alone causes little or no effect on an intact gastric mucosa in the rat, that either intact organisms or bacteria-free filtrates cause similar prolongation and delayed healing of pre-existing ulcers with active chronic inflammation, and that the presence of predisposing factors leading to disruption of gastric mucosal integrity may be required for the H. pylori enhancement of inflammation and tissue damage in the stomach.
(5) The molecular weight of antigen RFB2 was estimated to be approximately 85,000 daltons based on the results of gel filtration on Sepharose CL-6B.
(6) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
(7) The flow properties of white cells were tested after myocardial infarction, by measuring the filtration rates of cell suspensions through 8 microns pore filters.
(8) Slight cross-reactivity was apparent when crude preparations of cellular or culture filtrate antigens, used in this laboratory to detect antibodies to Candida albicans, Coccidioides immitis and Cryptococcus neoformans, were probed with hyperimmune rabbit antisera to A. fumigatus.
(9) The culture filtrate and OM preparation were capable of inhibiting the chemotaxis of PMNL in response to the chemotactic factors of E. coli but LPS of B. fragilis was not able to do so.
(10) The enzyme from rat lung was purified about 30-fold by pH treatment (NH4)2SO4 fractionation, and gel filtration.
(11) Ten filtrating blebs remained after a 5 months' follow-up period.
(12) Medium molecules have been detected by two methods, gel filtration and screening technique, in patients with diffuse purulent peritonitis and with chronic renal insufficiency.
(13) Even though the administration of demethylchlortetracycline did not produce significant decreases in the glomerular filtration rate or renal blood flow in our patient, it is advisable to control the renal function in individuals treated with this drug since it may on occasion determine renal insufficiency.
(14) The fucose-labeled glycoproteins were converted to glycopeptides by pronase digestion and separated into two major classes by gel filtration on Sephadex-G-50.
(15) Receptors solubilized with Triton X-100 eluted in gel filtration as a major peak of Mr = 330,000 and retained high affinity binding (KD = 0.25 nM).
(16) It is inferred that in this experimental model (1) high-density lipoproteins are probably excreted in the glomerular filtrate, (2) alterations in the composition of the excreted lipoproteins may occur during their passage through the nephron.
(17) Fractionation of uremic serum by gel filtration on Sephadex G-200 shows that the inhibitory activity elutes with an apparent molecular weight of greater than 200,000 daltons.
(18) Ratfish serum proteins have been fractionated by ammonium sulfate, followed by gel filtration on Sepharose 6B.
(19) In other dogfish, glomerular filtration rate, urine flow, and Na and K excretory rates were measured for 3 days following implantation of desoxycorticosterone (DOCA), adrenocorticotropin (ACTH), or spironolactone; a control group was given no drug.
(20) An isolation technique involving filtration and discontinuous density gradient centrifugation was utilized for obtaining Giardia lamblia cysts from human feces.
Percolation
Definition:
(n.) The act or process of percolating, or filtering; filtration; straining. Specifically (Pharm.), the process of exhausting the virtues of a powdered drug by letting a liquid filter slowly through it.
Example Sentences:
(1) As soon as the component with the lower mechanical stability is percolating the powder system, tablet hardness is controlled entirely by this component.
(2) This group consisted of 101 cases of whom 38 underwent semen treatment with Centrifugation on a Discontinuous Percoll Gradient (CDPG) and 63 with Pellet Swim-up (PS); the control group was made up of 31 normospermic patients where the semen was treated by PS.
(3) The attempt to fractionate the testicular cells by centrifugation in the continuous and discontinuous Percoll gradient was undertaken.
(4) The platelet subpopulations were separated with discontinuous gradients of Percoll.
(5) By using Percoll discontinuous density gradient centrifugation, peripheral blood nonphagocytic and nonadherent mononuclear cells were divided into the low and high density fractions for which natural killer (NK) cells and T cells were enriched, respectively.
(6) We conclude that both localized memory and percolation are possible in stimulatory idiotypic networks.
(7) The effect of activated PMN was tested on the motility of Percoll-washed spermatozoa in the presence and absence of reactive oxygen species scavengers or seminal plasma (whole or fractionated).
(8) To cope with this problem, in 27 IVF cycles, sperm selection was performed by centrifugation on discontinuous Percoll density gradient.
(9) Thirdly, the discontinuous percoll density gradient centrifugation was used to separate P. carinii cysts.
(10) Fractionation by Percoll density centrifugation of peripheral blood leucocyte cells, from atopic subjects with seasonal hay fever, unmasked IgE-B cell populations whose individual capacities to synthesize IgE in vitro were obscured in cultures of unfractionated B cells.
(11) Inspection of the cavity margins revealed absence of percolation at the dentin margins.
(12) In general, IEL of satisfactory yield and of good viability were obtained with EDTA treatment of the gut tissues, followed by rapid passages of the resultant cells through nylon-wool columns and centrifugation on two-step Percoll density gradients (45% and 80%).
(13) Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes.
(14) Percoll-purified high density small lymphocytes had little or no migratory capacity under these conditions, requiring a longer incubation time (4 hr) for consistent migration.
(15) Guinea pig marrow cell suspensions were first enriched for megakaryocytes by density equilibrium centrifugation in continuous Percoll density gradients.
(16) Using Percoll density gradient centrifugation, 1,25(OH)2D3 receptors could be localized in the basal cell fraction.
(17) After introduction of erythrocyte targets, there was a 20- to 30-min delay before initiation of phagocytosis that was not observed with monocytes prepared by the standard Percoll-gradient technique.
(18) To accomplish this, blood was obtained from patients with ragweed AR, granulocytes were isolated and fractionated by continuous density Percoll gradients, and the density distribution of these cells was determined after centrifugation.
(19) A cellular compartment from brown adipose tissue (BAT) of newborn rats was isolated by Percoll-density-gradient centrifugation and was shown to proliferate and to undergo adipose conversion in vitro in primary culture.
(20) Collagenase-dispersed cells from human chorion laeve were examined on Percoll gradients.