(n.) Any luminous winged insect, esp. luminous beetles of the family Lampyridae.
Example Sentences:
(1) A 1,300-base pair fragment of 5'-flanking sequence containing these elements, ligated upstream from a firefly luciferase reporter gene and transfected into cultured normal rat kidney cells, demonstrated strong promoter activity.
(2) Responding quickly, whatever the channel, is one of the most important things when it comes to how happy clients feel about the interaction they’ve had,” said Simon Hay, co-founder of online learning platform Firefly .
(3) Two genes, uidA encoding beta-glucuronidase (GUS) from Escherichia coli and Luc, encoding firefly luciferase (LUC), were used to analyze the ability of a cap, polyadenylated tail, and the 5'- and 3'-untranslated regions (UTR) from tobacco mosaic virus (TMV) to regulate expression.
(4) The method is based on the expression of an N-terminally extended firefly luciferase gene which requires frameshifting in order to be translated as a functional enzyme.
(5) A bioluminescent indicator for protein kinase A has been developed by mutating V217 in firefly (Photinus pyralis) luciferase to R, and the C-terminal peroxisomal signal removed by PCR.
(6) ATP remaining in the sample after incubation was extracted in boiling buffer and assayed by the firefly luciferase assay.
(7) ATP synthase and ATPase activities of the particles were measured in similar reaction mixtures by luminescence of firefly luciferin-luciferase.
(8) The Cre-lox site-specific recombination system of bacteriophage P1 was used to excise a firefly luciferase (luc) gene which had previously been incorporated into the tobacco genome.
(9) The effects of substrates (ATP and luciferin) on stability of firefly luciferase embedded into phosphatidylcholine liposomes have been studied.
(10) The endogenous release of adenosine 5'-triphosphate (ATP) from strips of guinea-pig gallbladder during transmural stimulation (TS) was measured with a firefly luciferine-luciferase reaction.
(11) A cDNA encoding the firefly luciferase [Photinus luciferin: oxygen 4-oxidoreductase (decarboxylating, ATP-hydrolyzing), EC 1.13.12.7] was cloned downstream from the polyhedrin gene promoter of Autographa californica nuclear polyhedrosis virus and expressed in Spodoptera frugiperda clone-9 cells.
(12) Recent advances in the analytical applications of bacterial and firefly luciferases and firefly luciferin are reviewed.
(13) The full-length, intronless luciferase gene was inserted into mammalian expression vectors and introduced into monkey (CV-1) cells in which enzymatically active firefly luciferase was transiently expressed.
(14) Hay, from Firefly, said he uses two types of survey in his business: a “How do you feel about our product?” survey that is sent out a few times each year, giving customers the chance to suggest ideas, questions and problems.
(15) Using a very sensitive firefly biolumnescence method 6% of the normal content of Ap4A was, however, found in platelets from sick animals.
(16) Fusion constructs containing 5'-flanking sequences from the rat PRL gene linked to either the bacterial chloramphenicol acetyltransferase (CAT) gene or the firefly luciferase gene were transiently expressed in GH3 cells and the transcriptional response to Ca2+ channel modulators was assessed.
(17) These analogues are the first examples of bioluminescent substrates for firefly luciferase that do not contain a benzothiazole moiety.
(18) This is quite obvious in the firefly where the energy, required for light production, is derived from ATP and in a couple of strains of luminescent bacteria where reduced pyridine nucleotides through reduction of flavine mononucleotide is utilized in the light reaction.
(19) The ATP concentration of intact, cold-tolerant (ground squirrel) red cells and cold-sensitive (guinea-pig and human) red cells was monitored by use of the firefly tail, luciferin-luciferase assay.
(20) The enzymatic activity of firefly luciferase provides a sensitive, rapid means to assay transcriptional activity of regulated activation sequences of DNA when fused to the protein coding sequence of the luciferase gene.
Glowworm
Definition:
(n.) A coleopterous insect of the genus Lampyris; esp., the wingless females and larvae of the two European species (L. noctiluca, and L. splendidula), which emit light from some of the abdominal segments.
Example Sentences:
(1) For all its posing and grooming, there are no nightclubs - the only flashing lights along this coast are the glowworms strobing across the grass at dusk.