(n.) Any luminous winged insect, esp. luminous beetles of the family Lampyridae.
Example Sentences:
(1) A 1,300-base pair fragment of 5'-flanking sequence containing these elements, ligated upstream from a firefly luciferase reporter gene and transfected into cultured normal rat kidney cells, demonstrated strong promoter activity.
(2) Responding quickly, whatever the channel, is one of the most important things when it comes to how happy clients feel about the interaction they’ve had,” said Simon Hay, co-founder of online learning platform Firefly .
(3) Two genes, uidA encoding beta-glucuronidase (GUS) from Escherichia coli and Luc, encoding firefly luciferase (LUC), were used to analyze the ability of a cap, polyadenylated tail, and the 5'- and 3'-untranslated regions (UTR) from tobacco mosaic virus (TMV) to regulate expression.
(4) The method is based on the expression of an N-terminally extended firefly luciferase gene which requires frameshifting in order to be translated as a functional enzyme.
(5) A bioluminescent indicator for protein kinase A has been developed by mutating V217 in firefly (Photinus pyralis) luciferase to R, and the C-terminal peroxisomal signal removed by PCR.
(6) ATP remaining in the sample after incubation was extracted in boiling buffer and assayed by the firefly luciferase assay.
(7) ATP synthase and ATPase activities of the particles were measured in similar reaction mixtures by luminescence of firefly luciferin-luciferase.
(8) The Cre-lox site-specific recombination system of bacteriophage P1 was used to excise a firefly luciferase (luc) gene which had previously been incorporated into the tobacco genome.
(9) The effects of substrates (ATP and luciferin) on stability of firefly luciferase embedded into phosphatidylcholine liposomes have been studied.
(10) The endogenous release of adenosine 5'-triphosphate (ATP) from strips of guinea-pig gallbladder during transmural stimulation (TS) was measured with a firefly luciferine-luciferase reaction.
(11) A cDNA encoding the firefly luciferase [Photinus luciferin: oxygen 4-oxidoreductase (decarboxylating, ATP-hydrolyzing), EC 1.13.12.7] was cloned downstream from the polyhedrin gene promoter of Autographa californica nuclear polyhedrosis virus and expressed in Spodoptera frugiperda clone-9 cells.
(12) Recent advances in the analytical applications of bacterial and firefly luciferases and firefly luciferin are reviewed.
(13) The full-length, intronless luciferase gene was inserted into mammalian expression vectors and introduced into monkey (CV-1) cells in which enzymatically active firefly luciferase was transiently expressed.
(14) Hay, from Firefly, said he uses two types of survey in his business: a “How do you feel about our product?” survey that is sent out a few times each year, giving customers the chance to suggest ideas, questions and problems.
(15) Using a very sensitive firefly biolumnescence method 6% of the normal content of Ap4A was, however, found in platelets from sick animals.
(16) Fusion constructs containing 5'-flanking sequences from the rat PRL gene linked to either the bacterial chloramphenicol acetyltransferase (CAT) gene or the firefly luciferase gene were transiently expressed in GH3 cells and the transcriptional response to Ca2+ channel modulators was assessed.
(17) These analogues are the first examples of bioluminescent substrates for firefly luciferase that do not contain a benzothiazole moiety.
(18) This is quite obvious in the firefly where the energy, required for light production, is derived from ATP and in a couple of strains of luminescent bacteria where reduced pyridine nucleotides through reduction of flavine mononucleotide is utilized in the light reaction.
(19) The ATP concentration of intact, cold-tolerant (ground squirrel) red cells and cold-sensitive (guinea-pig and human) red cells was monitored by use of the firefly tail, luciferin-luciferase assay.
(20) The enzymatic activity of firefly luciferase provides a sensitive, rapid means to assay transcriptional activity of regulated activation sequences of DNA when fused to the protein coding sequence of the luciferase gene.
Nocturnal
Definition:
(a.) Of, pertaining to, done or occuring in, the night; as, nocturnal darkness, cries, expedition, etc.; -- opposed to diurnal.
(a.) Having a habit of seeking food or moving about at night; as, nocturnal birds and insects.
(n.) An instrument formerly used for taking the altitude of the stars, etc., at sea.
Example Sentences:
(1) We evaluated the circadian pattern of gastric acidity by prolonged intraluminal pHmetry in 15 "responder" and 10 "nonresponder" duodenal ulcer patients after nocturnal administration of placebo, ranitidine, and famotidine.
(2) Both treatments depressed nocturnal pineal melatonin content in rats and hamsters.
(3) Nocturnal ST segment changes were abolished in six patients on atenolol, in six patients on nifedipine, and in five patients on isosorbide mononitrate.
(4) Stage REM frequently appeared within 10 min of stage 1 onset and the normal sequence of stages REM and 4 were altered, demonstrating that the organization of sleep within a nap is quite different from that in monophasic nocturnal sleep.
(5) The drug proved to be of high value in alleviating nocturnal coughing controlling spastic bronchitis in children, as a pretreatment before bronchological examinations and their anaesthesia.
(6) A statistically significant difference (p less than 0.01) was found between salmeterol and the association for this criteria: during the first period, 46% of subjects treated by salmeterol did not present nocturnal awakenings during the last treatment week by comparison with 15% of subjects taking the association; during the second period, corresponding figures were 39% for salmeterol by comparison with 26% for the association.
(7) Results from studies show that there can be a general hangover the morning following nocturnal doses of 2 mg.
(8) Nafarelin also allows assessment of the bioactivity of endogenous gonadotropin, is a more potent stimulus of pituitary-testicular function than endogenous GnRH secretion, and is more cost-effective than nocturnal sampling.
(9) Nocturnal penile tumescence results correlated well with the angiographic picture.
(10) One type of short-axon horizontal cell (HC) and one type of axonless HC are described in the retina of Carinae noctua, a crepuscular bird and Tyto alba, a pure nocturnal bird.
(11) When administered to adult patients with urge incontinence (generally as a 25mg twice-daily dose) terodiline reduces diurnal and nocturnal micturition frequency and incontinence episodes.
(12) To determine what effect higher nocturnal STC would have in patients with chronic obstructive pulmonary disease (COPD) on overnight lung function, oxygen saturation, and sleep quality, two different theophylline products were used to give higher or lower STC during the night.
(13) A diagnosis of paroxysmal nocturnal hemoglobinuria may be suggested with magnetic resonance imaging, based on the massive renal cortical hemosiderosis that occurs in this disease.
(14) No IgE circadian rhythm was validated in healthy children while a large amplitude (approximately equal to 30% of the 24 hours mean) circadian rhythm with 2 diurnal peaks and a nocturnal trough was demonstrated (P less than 0.0023) in the asthmatics.
(15) These results extend the scope of immunologic circadian rhythms to the reticuloendothelial system as a feature of a bioperiodic defense mechanism, most active during the habitual rest light span of nocturnally active mice.
(16) The degree of change was comparable during the diurnal and nocturnal periods.
(17) The administration of vitamin E, a natural antioxidant, to a patient with paroxysmal nocturnal haemoglobinuria (PNH) failed to diminish the urinary excretion of 59-Fe as monitored by 59-Fe whole body counting and urinary loss of isotope.
(18) Sleep percentages were higher when recordings were done during the nocturnal period.
(19) In comparison with age-matched normal controls, the fragile-X group showed lower melatonin values and a significant impairment of the nocturnal rise in this hormone.
(20) Accordingly, the effect of alpha-adrenergic stimulation and anticholinergic suppression was found to be insufficient to achieve nocturnal continence in patients with ileocaecal bladder replacement.