What's the difference between fluorescein and indicator?

Fluorescein


Definition:

  • (n.) A yellowish red, crystalline substance, C20H12O5, produced by heating together phthalic anhydride and resorcin; -- so called, from the very brilliant yellowish green fluorescence of its alkaline solutions. It has acid properties, and its salts of the alkalies are known to the trade under the name of uranin.

Example Sentences:

  • (1) We have evaluated the life-span of B lymphocytes by measuring the functional reactivity of normal B cells upon transfer into xid mice, which do not respond to anti-mu, fluoresceinated-Ficoll (FL-Ficoll) and 2,4,6-trinitrophenyl aminoethylcarbamylmethyl Ficoll (TNP-Ficoll).
  • (2) In 60 rhesus monkeys with experimental renovascular malignant arterial hypertension (25 one-kidney and 35 two-kidney model animals), we studied the so-called 'hard exudates' or white retinal deposits in detail (by ophthalmoscopy, and stereoscopic color fundus photography and fluorescein fundus angiography, on long-term follow-up).
  • (3) Fluorescein isothiocyanate (FITC)-ricin exhibited binding to schistosomula and adult worms, but not to cercariae or to freshly transformed schistosomula.
  • (4) The greater biliary excretion and hepatic extraction of cholyl-lys-F relative to free fluorescein further suggest that conjugation with a bile salt may be an efficient way of targeting compounds to the liver.
  • (5) Fluorescein-coupled peanut agglutinin (PNA) has been used at the single-cell level to study mouse lymphocyte subpopulations.
  • (6) Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, biochemical, and serological procedures.
  • (7) Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA.
  • (8) The DNA and protein contents of isolated basal cells were stained with propidium iodide and fluorescein isothiocyanate, respectively, and analysed by flow cytometry using the total protein fluorescence as an estimate of cell size and the DNA fluorescence pulse width as an estimate of nuclear size.
  • (9) Fluorescein-labelled gamma globulin fractions of anti-avidin and anti-uteroglobin both bound to blastocysts, but pure FITC-IgG showed binding only of anti-uteroglobin.
  • (10) The differences between periodate- and NAGO-induced aldehydes were demonstrated by flow cytometry of cells stained with a novel fluoresceinated hydrazide and by an electrophoretic procedure performed with biocytin hydrazide and 125I-streptavidin.
  • (11) For this, rabbit anti-chicken globulin fluorescein isothiocyanate conjugate was used.
  • (12) Active transport of fluorescein across the blood-retinal barrier in the direction of vitreous to blood does not seem to be significant within the first 2 hr after fluorescein injection.
  • (13) The treatment was almost only in those angiopathies successful, in which the fluorescein angiography showed a preponderance of the hyperpermeability over the obliterating process of retinal capillaries.
  • (14) Survivability was judged by morphological appearance, trypan blue exclusion and fluorescein diacetate staining.
  • (15) Cytofluorographic analyses confirmed the increased expression of high-affinity FcR, binding fluoresceinating murine IgG2a.
  • (16) Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry.
  • (17) Indirect immunofluorescence microscopy with fluorescein isothiocyanate-anti-mouse immunoglobulin G also identified the antigen on the surface of cultured and fresh tumor cells and detected the antigen on 16 of 17 Grade 3 bladder tumor specimens as well as on some kidney and testicular tumor cells.
  • (18) The expression of EBVR was established by the binding of fluorescein isothiocyanate (FITC)-labeled EBV to the cells, while surface markers were stained by phycoerythrin (PE)-indirect immunofluorescence, using monoclonal antibodies.
  • (19) IC50 values of 6.8.10(-9), 1.7.10(-8), 1.8.10(-8) and 5.4.10(-9) M were measured in rat liver membranes for NBD-, fluorescein-, rhodamine-Trp25-glucagon and native glucagon, respectively.
  • (20) Sodium fluorescein and fluorescinated dextrans (FD) of selected molecular weights were combined and perfused into the anterior chamber of normal and inflamed eyes of cynomolgus monkeys.

Indicator


Definition:

  • (n.) One who, or that which, shows or points out; as, a fare indicator in a street car.
  • (n.) A pressure gauge; a water gauge, as for a steam boiler; an apparatus or instrument for showing the working of a machine or moving part
  • (n.) An instrument which draws a diagram showing the varying pressure in the cylinder of an engine or pump at every point of the stroke. It consists of a small cylinder communicating with the engine cylinder and fitted with a piston which the varying pressure drives upward more or less against the resistance of a spring. A lever imparts motion to a pencil which traces the diagram on a card wrapped around a vertical drum which is turned back and forth by a string connected with the piston rod of the engine. See Indicator card (below).
  • (n.) A telltale connected with a hoisting machine, to show, at the surface, the position of the cage in the shaft of a mine, etc.
  • (n.) The part of an instrument by which an effect is indicated, as an index or pointer.
  • (n.) Any bird of the genus Indicator and allied genera. See Honey guide, under Honey.
  • (n.) That which indicates the condition of acidity, alkalinity, or the deficiency, excess, or sufficiency of a standard reagent, by causing an appearance, disappearance, or change of color, as in titration or volumetric analysis.

Example Sentences:

  • (1) Indicators for evaluation and monitoring and outcome measures are described within the context of health service management to describe control measure output in terms of community effectiveness.
  • (2) These data indicate a steady improvement in laboratory performance over the last 10 years.
  • (3) Isotope competition studies indicated that the pathway was regulated by isoleucine.
  • (4) The results indicated that neuropsychological measures may serve to broaden the concept of intelligence and that a brain-related criterion may contribute to a fuller understanding of its nature.
  • (5) These results indicated that the PG determination was the most accurate predictor of fetal lung well-being prior to birth among the clinical tests so far reported.
  • (6) The findings indicate that there is still a significant incongruence between the value structure of most family practice units and that of their institutions but that many family practice units are beginning to achieve parity of promotion and tenure with other departments in their institutions.
  • (7) The predicted non-Lorentzian line shapes and widths were found to be in good agreement with experimental results, indicating that the local orientational order (called "packing" by many workers) in the bilayers of small vesicles and in multilamellar membranes is substantially the same.
  • (8) These studies led to the following conclusions: (a) all the prominent NHP which remain bound to DNA are also present in somewhat similar proportions in the saline-EDTA, Tris, and 0.35 M NaCl washes of nuclei; (b) a protein comigrating with actin is prominent in the first saline-EDTA wash of nuclei, but present as only a minor band in the subsequent washes and on washed chromatin; (c) the presence of nuclear matrix proteins in all the nuclear washes and cytosol indicates that these proteins are distributed throughout the cell; (d) a histone-binding protein (J2) analogous to the HMG1 protein of K. V. Shooter, G.H.
  • (9) Electrophysiologic studies are indicated in patients with sustained paroxysmal ventricular tachycardia, ventricular fibrillation or aborted sudden death.
  • (10) In cardiac tissue the adenylate system is not a good indicator of the energy state of the mitochondrion, even when the concentrations of AMP and free cytosolic ADP are calculated from the adenylate kinase and creatine kinase equilibria.
  • (11) Comparison of wild type and the mutant parD promoter sequences indicated that three short repeats are likely involved in the negative regulation of this promoter.
  • (12) Results indicated a .85 probability that Directive Guidance would be followed by Cooperation; a .67 probability that Permissiveness would lead to Noncooperation; and a .97 likelihood that Coerciveness would lead to either Noncooperation or Resistance.
  • (13) The data from this experience as well as others previously reported can yield prognostic indicators of survival in cases of accidental hypothermia.
  • (14) The data indicate that ebselen is likely to be useful in the therapy of inflammatory conditions in which reactive oxygen species, such as peroxides, play an aetiological role.
  • (15) This induction is sensitive to actinomycin D but not to protein synthesis inhibitor puromycin, indicating an effect of estradiol at the transcriptional level, possibly mediated by the estrogen receptor.
  • (16) Quantitative determinations indicate that the amount of PBG-D mRNA is modulated both by the erythroid nature of the tissue and by cell proliferation, probably at the transcriptional level.
  • (17) A disease in an IgD (lambda) plasmocytoma is described, where after therapy with Alkeran and prednisone a disappearance of all clinical and laboratory findings indicating an activity could be observed.
  • (18) Our results indicate that increasing the delay for more than 8 days following irradiation and TCD syngeneic BMT leads to a rapid loss of the ability to achieve alloengraftment by non-TCD allogeneic bone marrow.
  • (19) gamma-Aminobutyric acid (GABA) and glutamate release from the treated side was higher than the control value during the first 2-3 h, a result indicating an important role of glial cells in the inactivation of released transmitter.
  • (20) These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.