What's the difference between fluorescein and resorcinolphthalein?
Fluorescein
Definition:
(n.) A yellowish red, crystalline substance, C20H12O5, produced by heating together phthalic anhydride and resorcin; -- so called, from the very brilliant yellowish green fluorescence of its alkaline solutions. It has acid properties, and its salts of the alkalies are known to the trade under the name of uranin.
Example Sentences:
(1) We have evaluated the life-span of B lymphocytes by measuring the functional reactivity of normal B cells upon transfer into xid mice, which do not respond to anti-mu, fluoresceinated-Ficoll (FL-Ficoll) and 2,4,6-trinitrophenyl aminoethylcarbamylmethyl Ficoll (TNP-Ficoll).
(2) In 60 rhesus monkeys with experimental renovascular malignant arterial hypertension (25 one-kidney and 35 two-kidney model animals), we studied the so-called 'hard exudates' or white retinal deposits in detail (by ophthalmoscopy, and stereoscopic color fundus photography and fluorescein fundus angiography, on long-term follow-up).
(3) Fluorescein isothiocyanate (FITC)-ricin exhibited binding to schistosomula and adult worms, but not to cercariae or to freshly transformed schistosomula.
(4) The greater biliary excretion and hepatic extraction of cholyl-lys-F relative to free fluorescein further suggest that conjugation with a bile salt may be an efficient way of targeting compounds to the liver.
(5) Fluorescein-coupled peanut agglutinin (PNA) has been used at the single-cell level to study mouse lymphocyte subpopulations.
(6) Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, biochemical, and serological procedures.
(7) Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA.
(8) The DNA and protein contents of isolated basal cells were stained with propidium iodide and fluorescein isothiocyanate, respectively, and analysed by flow cytometry using the total protein fluorescence as an estimate of cell size and the DNA fluorescence pulse width as an estimate of nuclear size.
(9) Fluorescein-labelled gamma globulin fractions of anti-avidin and anti-uteroglobin both bound to blastocysts, but pure FITC-IgG showed binding only of anti-uteroglobin.
(10) The differences between periodate- and NAGO-induced aldehydes were demonstrated by flow cytometry of cells stained with a novel fluoresceinated hydrazide and by an electrophoretic procedure performed with biocytin hydrazide and 125I-streptavidin.
(11) For this, rabbit anti-chicken globulin fluorescein isothiocyanate conjugate was used.
(12) Active transport of fluorescein across the blood-retinal barrier in the direction of vitreous to blood does not seem to be significant within the first 2 hr after fluorescein injection.
(13) The treatment was almost only in those angiopathies successful, in which the fluorescein angiography showed a preponderance of the hyperpermeability over the obliterating process of retinal capillaries.
(14) Survivability was judged by morphological appearance, trypan blue exclusion and fluorescein diacetate staining.
(15) Cytofluorographic analyses confirmed the increased expression of high-affinity FcR, binding fluoresceinating murine IgG2a.
(16) Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry.
(17) Indirect immunofluorescence microscopy with fluorescein isothiocyanate-anti-mouse immunoglobulin G also identified the antigen on the surface of cultured and fresh tumor cells and detected the antigen on 16 of 17 Grade 3 bladder tumor specimens as well as on some kidney and testicular tumor cells.
(18) The expression of EBVR was established by the binding of fluorescein isothiocyanate (FITC)-labeled EBV to the cells, while surface markers were stained by phycoerythrin (PE)-indirect immunofluorescence, using monoclonal antibodies.
(19) IC50 values of 6.8.10(-9), 1.7.10(-8), 1.8.10(-8) and 5.4.10(-9) M were measured in rat liver membranes for NBD-, fluorescein-, rhodamine-Trp25-glucagon and native glucagon, respectively.
(20) Sodium fluorescein and fluorescinated dextrans (FD) of selected molecular weights were combined and perfused into the anterior chamber of normal and inflamed eyes of cynomolgus monkeys.