What's the difference between fluorescein and uranin?
Fluorescein
Definition:
(n.) A yellowish red, crystalline substance, C20H12O5, produced by heating together phthalic anhydride and resorcin; -- so called, from the very brilliant yellowish green fluorescence of its alkaline solutions. It has acid properties, and its salts of the alkalies are known to the trade under the name of uranin.
Example Sentences:
(1) We have evaluated the life-span of B lymphocytes by measuring the functional reactivity of normal B cells upon transfer into xid mice, which do not respond to anti-mu, fluoresceinated-Ficoll (FL-Ficoll) and 2,4,6-trinitrophenyl aminoethylcarbamylmethyl Ficoll (TNP-Ficoll).
(2) In 60 rhesus monkeys with experimental renovascular malignant arterial hypertension (25 one-kidney and 35 two-kidney model animals), we studied the so-called 'hard exudates' or white retinal deposits in detail (by ophthalmoscopy, and stereoscopic color fundus photography and fluorescein fundus angiography, on long-term follow-up).
(3) Fluorescein isothiocyanate (FITC)-ricin exhibited binding to schistosomula and adult worms, but not to cercariae or to freshly transformed schistosomula.
(4) The greater biliary excretion and hepatic extraction of cholyl-lys-F relative to free fluorescein further suggest that conjugation with a bile salt may be an efficient way of targeting compounds to the liver.
(5) Fluorescein-coupled peanut agglutinin (PNA) has been used at the single-cell level to study mouse lymphocyte subpopulations.
(6) Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, biochemical, and serological procedures.
(7) Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA.
(8) The DNA and protein contents of isolated basal cells were stained with propidium iodide and fluorescein isothiocyanate, respectively, and analysed by flow cytometry using the total protein fluorescence as an estimate of cell size and the DNA fluorescence pulse width as an estimate of nuclear size.
(9) Fluorescein-labelled gamma globulin fractions of anti-avidin and anti-uteroglobin both bound to blastocysts, but pure FITC-IgG showed binding only of anti-uteroglobin.
(10) The differences between periodate- and NAGO-induced aldehydes were demonstrated by flow cytometry of cells stained with a novel fluoresceinated hydrazide and by an electrophoretic procedure performed with biocytin hydrazide and 125I-streptavidin.
(11) For this, rabbit anti-chicken globulin fluorescein isothiocyanate conjugate was used.
(12) Active transport of fluorescein across the blood-retinal barrier in the direction of vitreous to blood does not seem to be significant within the first 2 hr after fluorescein injection.
(13) The treatment was almost only in those angiopathies successful, in which the fluorescein angiography showed a preponderance of the hyperpermeability over the obliterating process of retinal capillaries.
(14) Survivability was judged by morphological appearance, trypan blue exclusion and fluorescein diacetate staining.
(15) Cytofluorographic analyses confirmed the increased expression of high-affinity FcR, binding fluoresceinating murine IgG2a.
(16) Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry.
(17) Indirect immunofluorescence microscopy with fluorescein isothiocyanate-anti-mouse immunoglobulin G also identified the antigen on the surface of cultured and fresh tumor cells and detected the antigen on 16 of 17 Grade 3 bladder tumor specimens as well as on some kidney and testicular tumor cells.
(18) The expression of EBVR was established by the binding of fluorescein isothiocyanate (FITC)-labeled EBV to the cells, while surface markers were stained by phycoerythrin (PE)-indirect immunofluorescence, using monoclonal antibodies.
(19) IC50 values of 6.8.10(-9), 1.7.10(-8), 1.8.10(-8) and 5.4.10(-9) M were measured in rat liver membranes for NBD-, fluorescein-, rhodamine-Trp25-glucagon and native glucagon, respectively.
(20) Sodium fluorescein and fluorescinated dextrans (FD) of selected molecular weights were combined and perfused into the anterior chamber of normal and inflamed eyes of cynomolgus monkeys.
Uranin
Definition:
(n.) An alkaline salt of fluorescein, obtained as a brownish red substance, which is used as a dye; -- so called from the peculiar yellowish green fluorescence (resembling that of uranium glass) of its solutions. See Fluorescein.
Example Sentences:
(1) In experiments on irradiated (10 Gy) mice a study was made of the morphological changes in the small intestine mucosa and in its functional status estimated by absorption of stain uranine.
(2) A mixture of a cell culture-adapted strain (C-486) of calf rotavirus and poliovirus type 1 (Sabin) was prepared in tryptose phosphate broth containing 0.1% uranine (physical tracer) and antifoam at a final concentration of 0.001%.
(3) At an early stage of the disease (6-24 hours) insufficient was uranin fluorescence in blood plasma, but more informative were the changes in adhesive properties of leukocytes the dynamics of lymphocytes (lymphopenia), reticulocytes (reticulocytopenia) and shifts in reticulograms (increased per cent of juvenile forms).
(4) In the experiments performed on kidneys of 5 species of marine Teleostei, morphological peculiarities in secretion of 8 fluorescent organic acids (uranin, primulin, tripaphlavin, erythrosin etc.)
(5) Laboratory tests with aerosolized spores and animal room tests with uranine dye indicate the effectiveness of a prototype bedding disposal cabinet in reducing airborne contamination generated by cage cleaning procedures.
(6) The strongest protection was elicited with acriflavine-, auramine O-, eosin Y-, neutral red-, night blue-, patent blue V-, thymol blue-, uranin-, and xylene cyanol FF-treated vaccines.
(7) This technique was assessed using the fluorescent exogenous agent uranin (fluorescein sodium).
(8) A multiple regression equation was developed from data obtained with uranine dye to define the aerosol concentration of the dye in the system as a function of the concentration of the dye in the spray fluid and the rate at which it was aerosolized.
(9) The authors have studied the influence of rose bengal, and, by comparison, of uranin, on choleresis in the rabbit.
(10) Intensification of lumininescence by the addition of uranin, eosin, tetrachlorfluorescein and erythrozan was found.
(11) It is assumed that fluorescein and uranin transports are carried out by producing a four-fold transporting complex (a carrier+a substratum+2Na+; in the case of fluorescein 2Na+ appear from the medium, whereas in the case of uranin only one Na+ from the medium is used.
(12) Aerosol deposited in the "T" and elbow, endotracheal tube, large airway model, and collection bag was quantitated separately using 0.1% uranine as a tracer.
(13) The process of luminescence intensification with the addition of uranine was thoroughly studied (kinetic regularities, spectra, concentration relationships).
(14) Administration of furosemide before disconnection of the renal circulation is accompanied by an increase of uranine transport from the nephron lumen to the blood as compared with the control.
(15) The KmNa value for fluorescein transport is twice as much as that for uranin transport.
(16) From the fact that uranine had the lower acute toxicity than halogens substituted compounds and the toxicities of these 4 dyes increased through irradiation, it was assumed that halogen atoms in dyes might be responsible for these strong acute toxicities to fish.
(17) This success rate was comparable to those for Evans blue, uranin or neutral red in this test system.
(18) The instrument was calibrated using uranine test aerosols, photofluorescence and optical microscopy techniques.
(19) It has been established in acute experiments on rats that furosemide reduces the supply of uranine from the lumen of renal tubules to the interstitial space and systemic blood flow.
(20) All the substances studied accumulate in the cell and only some of them (uranin, primulin, titanic yellow, etc.)