(1) The assembly reaction is accompanied by characteristic changes in fluorescence emission and dichroic absorption.
(2) Such an increase in antibody binding occurred simultaneously with an increase in the fluidity of surface lipid regions, as monitored by fluorescence depolarization of 1-(trimethylammoniophenyl)-6-phenyl-1,3,5-hexatriene.
(3) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
(4) The sensitivity of an indirect fluorescent antibody (IFA) test (screening test) for the detection of antibodies to cytomegalovirus (CMV) was examined by using 128 serum specimens and quaternary aminoethyl (QAE)-Sephadex A50 column chromatography to separate IgM from IgG class antibodies.
(5) Absorbance or fluorescence measurements may be used for detection.
(6) In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double-staining immunocytochemical methods employing highly specific antibodies to localize the following peptide-containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon.
(7) Likewise, they had little or no effects on the fluorescence anisotropy of TMA-DPH, which is also thought to be located in the interfacial region of the lipid bilayer, either when the probe was located in the outer layer of the plasma membrane or when the probe was located in the inner membrane compartment.
(8) Furthermore, experiments with the fluorescence-activated cell sorter revealed increased forward light scatter from resting exudate PMN compared to blood PMN.
(9) Sulphuric acid fluorescence is used for quantitation and specificity is achieved by the addition of tritiated oestrone to the urine hydrolysate.
(10) At the light-microscopic level, adrenergic fibres were identified due to their formaldehyde-induced fluorescence.
(11) Equilibrium and kinetic studies of the interaction of gene 32 protein of T4 phage with single-stranded fd DNA were performed monitoring the changes in protein fluorescence.
(12) The drug is extracted from serum or urine with ethyl acetate, separated by TLC, and determined by fluorescence quenching densitometry.
(13) In all these subjects, fluorescent staining and G-banding on chromosomes from cultured leukocytes confirmed their karyotype.
(14) Vital staining of neuroblastoma cells with acridine orange produces a bright intracellular red-orange fluorescence most probably due to the occurrence of RNA.
(15) Time-resolved tyrosine fluorescence anisotropy shows global correlation times broadly in agreement with the NMR results, but with an additional faster correlation time [approximately 600 ps].
(16) The specific fluorescence was affected following reserpine or 6-hydroxydopamine treatment; however, the rewarming process restored fluorescence only in the reserpine-treated tissue.
(17) I evaluated use of the fluorescence polarization technique to measure neocarzinostatin, a proteinaceous antitumor antibiotic, and its antibody, in serum.
(18) In the analysis of background fluorescence, the principal components were, as for the two-step technique, autofluorescence and propidium spectral overlap.
(19) Luminal and myoepithelial cells have been separated from normal adult human breast epithelium using fluorescence activated cell sorting.
(20) The influence of exogenous gangliosides on the structure of the viral envelope was studied using fluorescent and photoactivatable phospholipids incorporated into the viral membrane.
Labeling
Definition:
(p. pr. & vb. n.) of Label
Example Sentences:
(1) It was tested for recovery and separation from other selenium moieties present in urine using both in vivo-labeled rat urine and human urine spiked with unlabeled TMSe.
(2) Within the outflow tract wall, the labelled cells were enmeshed by strands of alcian blue-stained extracellular matrix.
(3) Competition with the labelled 10B12 MAb for binding to the purified antigen was demonstrated in sera of tumor-bearing and immune rats.
(4) Simplicity, high capacity, low cost and label stability, combined with relatively high clinical sensitivity make the method suitable for cost effective screening of large numbers of samples.
(5) Following central retinal artery ligation, infarction of the retinal ganglion cells was reflected by a 97 per cent reduction in the radioactively labeled protein within the optic nerve.
(6) Estimations of the degree of incorporation of 14C from the radioactive labeled carbohydrate into the glycerol and fatty acid moieties were carried out.
(7) In addition autoradiography was performed to localize labelled cells in the inner ear.
(8) When the data correlating DHT with protein synthesis using both labelling techniques were combined, the curves were parallel and a strong correlation was noted between DHT and protein synthesis over a wide range of values (P less than 0.001).
(9) Photoirradiation of F1 in the presence of the analog leads to inactivation depending linearly on the incorporation of label.
(10) Significant amounts of 35S-labeled material were lost during the alkali treatment.
(11) In all groups, there was a fall in labeling index with time reflecting increasing tumor size.
(12) In the measurement, enzyme-labeled and unlabeled antigens (Ag* and Ag) were allowed to compete in binding to the antibody (Ab) under conditions where Ag* much less than Ab much less than Ag.
(13) This mAb inhibited monocyte binding of both soluble FITC-labeled IgA and IgA-coated E, whereas it did not inhibit IgG binding.
(14) Autoradiographic studies with tritiated thymidine showed that both epithelial and mesenchymal tumor cells were labeled.
(15) However, when conjugated to an antigen-bearing cell, a "non-antigen bearing" cell was labeled near the cell interaction area.
(16) In oleate-labeled particles, besides phosphatidic acid the product of PLD action radioactivity was also detected in diglyceride as a result of resident phosphatidate phosphohydrolase, which hydrolyzed the phosphatidic acid.
(17) Intracellular localization of the labeled substance in the tumor tissue was examined autohistoradiographically.
(18) It could be demonstrated by radioimmune precipitation of virus labeled with[35S]methionine that all three polypeptides are specific for hog cholera virions.
(19) The labeling index of the treated groups was significantly reduced when compared to that of control group in both tumors.
(20) After absorption of labeled glucose, two pools of trehalose are found in dormant spores, one of which is extractable without breaking the spores, and the other, only after the spores are disintegrated.