(1) The assembly reaction is accompanied by characteristic changes in fluorescence emission and dichroic absorption.
(2) Such an increase in antibody binding occurred simultaneously with an increase in the fluidity of surface lipid regions, as monitored by fluorescence depolarization of 1-(trimethylammoniophenyl)-6-phenyl-1,3,5-hexatriene.
(3) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
(4) The sensitivity of an indirect fluorescent antibody (IFA) test (screening test) for the detection of antibodies to cytomegalovirus (CMV) was examined by using 128 serum specimens and quaternary aminoethyl (QAE)-Sephadex A50 column chromatography to separate IgM from IgG class antibodies.
(5) Absorbance or fluorescence measurements may be used for detection.
(6) In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double-staining immunocytochemical methods employing highly specific antibodies to localize the following peptide-containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon.
(7) Likewise, they had little or no effects on the fluorescence anisotropy of TMA-DPH, which is also thought to be located in the interfacial region of the lipid bilayer, either when the probe was located in the outer layer of the plasma membrane or when the probe was located in the inner membrane compartment.
(8) Furthermore, experiments with the fluorescence-activated cell sorter revealed increased forward light scatter from resting exudate PMN compared to blood PMN.
(9) Sulphuric acid fluorescence is used for quantitation and specificity is achieved by the addition of tritiated oestrone to the urine hydrolysate.
(10) At the light-microscopic level, adrenergic fibres were identified due to their formaldehyde-induced fluorescence.
(11) Equilibrium and kinetic studies of the interaction of gene 32 protein of T4 phage with single-stranded fd DNA were performed monitoring the changes in protein fluorescence.
(12) The drug is extracted from serum or urine with ethyl acetate, separated by TLC, and determined by fluorescence quenching densitometry.
(13) In all these subjects, fluorescent staining and G-banding on chromosomes from cultured leukocytes confirmed their karyotype.
(14) Vital staining of neuroblastoma cells with acridine orange produces a bright intracellular red-orange fluorescence most probably due to the occurrence of RNA.
(15) Time-resolved tyrosine fluorescence anisotropy shows global correlation times broadly in agreement with the NMR results, but with an additional faster correlation time [approximately 600 ps].
(16) The specific fluorescence was affected following reserpine or 6-hydroxydopamine treatment; however, the rewarming process restored fluorescence only in the reserpine-treated tissue.
(17) I evaluated use of the fluorescence polarization technique to measure neocarzinostatin, a proteinaceous antitumor antibiotic, and its antibody, in serum.
(18) In the analysis of background fluorescence, the principal components were, as for the two-step technique, autofluorescence and propidium spectral overlap.
(19) Luminal and myoepithelial cells have been separated from normal adult human breast epithelium using fluorescence activated cell sorting.
(20) The influence of exogenous gangliosides on the structure of the viral envelope was studied using fluorescent and photoactivatable phospholipids incorporated into the viral membrane.
Ultraviolet
Definition:
(a.) Lying outside the visible spectrum at its violet end; -- said of rays more refrangible than the extreme violet rays of the spectrum.
Example Sentences:
(1) Preincubation of the bacteria at 56 degrees C for 30 minutes and ultraviolet irradiation resulted in a noticeable decrease in adherence.
(2) Ultraviolet difference spectrophotometry indicates that the inactivated enzyme retains its capacity for binding the nucleotide substrates whereas the spectral perturbation characteristic of 3-phosphoglycerate binding is abolished in the modified enzyme.
(3) Allelic complementation was not observed, despite testing of a large number of allele pairs, and alleles suppressible by the ochre suppressor SUP11 were absent from a sample of 48 spontaneous mutants and occurred infrequently (7%) among a sample of ultraviolet-induced mutants.
(4) The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously.
(5) On the other hand, ultraviolet (320-nm) light, absorbed by 3-hydroxy-pyridinium cross-links which were rapidly photolyzed, partially dissociated polymeric collagen aggregates from bovine Achilles tendon after subsequent heating.
(6) Utilizing the bilateral comparison technique in 30 hospitalized patients with chronic stable plaque-type psoriasis vulgaris, we closely monitored the clinical responses to ultraviolet radiation (Westinghouse fluorescent FS40 bulbs, 290--400 nm) and a variety of tar preparations and lubricant vehicles in combination and separately.
(7) No new peak appears in the ultraviolet spectrum (240 approximately 300 nm) while mycobacillin is inactivated.
(8) Eyes exposed to ultraviolet radiation with their lenses intact were devoid of significant retinal lesions.
(9) We have investigated enhancement of pigmentation in inbred C3H- mice using tail skin as a model for testing the effects of phosphorylated DOPA (DP) and ultraviolet radiation.
(10) The method involves liquid-liquid extraction and HPLC with ultraviolet detection.
(11) Use of sunglasses that block all ultraviolet radiation and severely attenuate high-energy visible radiation will slow the pace of ocular deterioration and delay the onset of age-related disease, thereby reducing its prevalence.
(12) The effect of DNA-crosslinking agents (cisplatin and mitomycin C), a DNA-intercalating agent (adriamycin) or monofunctional psoralen (4-Met-4', 5'-dihydropsoralen plus near-ultraviolet radiation) on DNA replication in cultured mouse FM3A cells was studied by sedimentation of the pulse-labeled DNA in an alkaline sucrose gradient.
(13) Both arrested and growing cells exhibited an equal level of constitutive EPR following ultraviolet irradiation.
(14) Pyridinoline was isolated from the cross-linked peptide by preparative amino acid analysis and reversed-phase HPLC and identified by its ultraviolet absorption spectra, its fluorescence excitation and emission spectra and, for the first time, its time-of-flight secondary ion-mass spectrum.
(15) In aqueous solution at neutral pH, all the COOH-terminal fragments attain a native-like conformation, as judged both by the content of secondary structure deduced from far-ultraviolet CD spectra and by the recognition of rabbit polyclonal antibodies specific for the COOH-terminal region in native thermolysin.
(16) Affected individuals were not clinically photosensitive, but their fibroblasts demonstrated gross cytopathic changes, low survival indices and an increased frequency of DNA single strand breaks following exposure to long-wave ultraviolet radiation (UVA).
(17) One hand was treated with conventional superficial radiotherapy and the other with topical 8-methoxy-psoralen and long-wave ultraviolet light (topical PUVA).
(18) Upon binding to saccharides, ricin D displayed ultraviolet difference spectra with maxima at 280 nm and 288 nm.
(19) Monodispersed N- and C-protected linear homo-oligomethionines (n = 2- -7) are studied by measurements of circular dichroism in the vacuum ultraviolet region.
(20) Daughter-strand gaps in deoxyribonucleic acid (DNA) synthesized after exposure of excision-deficient Escherichia coli to ultraviolet light are filled during subsequent incubation in buffer, and the rate of filling is increased when the incubation in buffer is carried out in the presence of 360-nm light.