(v. t.) A part broken off; a small, detached portion; an imperfect part; as, a fragment of an ancient writing.
Example Sentences:
(1) The nucleotide sequence of a 2.2-kb DNA fragment which contains the complete RAD7 gene was determined.
(2) In the second approach, attachment sites of DTPA groups were directed away from the active region of the molecule by having fragment E1,2 bound in complex, with its active sites protected during the derivatization.
(3) The Fc fragment of this protein reacted with and was solubilized by the staphylococcal A protein which also precipitated the intact immunoglobulin.
(4) In order to determine the extent of this similarity, I have developed a panel of probes for many of the Pacl restriction fragments and have shown that most of the Pacl and Notl fragments found in MBa are also present in MBb.
(5) Under milder trypsin digestion conditions three resistant fragments were produced from the free protein.
(6) Five of them had a fast-moving Eco RI fragment 5.6 kb long that hybridized with zeta-specific probe but not with alpha-specific probe.
(7) Comparative data for the fragments from RNAs 1, 2, and 3 show that they have nearly the same sequence as the RNA 4 fragment.
(8) A sperm whale myoglobin gene containing multiple unique restriction sites has been constructed in pUC 18 by sequential assembly of chemically synthesized oligonucleotide fragments.
(9) Urine specimens from patient REE also contained a light chain fragment that lacked the first (amino-terminal) 85 residues of the native light chain but otherwise was identical in sequence to the light chain REE.
(10) Northern hybridization analysis of R. toruloides RNA with a restriction fragment encoding part of the PAL gene indicates that PAL mRNA is 2.5 kilobases in length.
(11) The method of sonicating L3 and Mf fragment antigens used in this study is simple, and its results are easy to observe.
(12) Benzyloxycarbonylarginine p-nitrophenyl ester and other activated esters of N-a-sustituted arginine salts may be useful reagents for introduction of trypsin-labile protecting groups into peptide fragments for purpose of polypeptide semi-synthesis.
(13) The 6.6-kb DNA Bam HI fragment containing the resistance gene was cloned with the plasmid vector pIJ699.
(14) It was found that these Hageman factor fragments promoted rapid proteolysis of one-chain factor VII to a more active two-chain form.
(15) Restriction fragment length polymorphisms (RFLPs) were studied in a large Algerian family which includes 6 haemophiliacs and a previously described case of female haemophilia A.
(16) A 2.7-kilobase DNA fragment carrying the entire exotoxin A (ETA) structural gene was divided into three nonoverlapping probes.
(17) A 6.4 kilobase C4B-5'-specific Taq I fragment usually provided a reliable guide to the presence of a C4A deletion but unusually in one instance this fragment was found to be a marker of a functioning C4A gene.
(18) The method involves the use of a monoclonal antibody fragment mixture that binds to platelets.
(19) Three distinct antigenic regions of bovine somatotropin (bST) were identified on the basis of the ability of a set of monoclonal antibodies to bind to proteolytic fragments and deletion variants of recombinant bST (rbST) in Western blot analyses.
(20) The coding sequence for Spirulina platensis acetohydroxy acid synthase (AHAS, EC 4.1.3.18) is shown to be contained within a 4.2 Kb ClaI fragment (ilvX) that has been cloned from a recombinant lambda library.
Recrystallization
Definition:
(n.) The process or recrystallizing.
Example Sentences:
(1) The recrystallized surface protein arrays gave a resolution of approximately 1.3 nm in projection after correlation averaging of negatively stained preparations.
(2) A synthetic precursor to a potent dopaminergic agonist, (RS)-4-hydroxy-2-(di-n-propylamino)indan, has been resolved by classical recrystallization procedures, and the absolute configurations of the enantiomers have been established by X-ray crystallographic analysis.
(3) Pure alpha-epimer was obtained by recrystallization and low pressure silica gel column chromatography combined with HPLC method.
(4) Microsomal and low speed pellets with added cofactors formed products which recrystallized with either etiocholanolone or androsterone from [3H]T or [3H]DHT, respectively.
(5) The blood-brain barrier in the rhesus monkey was opened to intravascular Evans blue-albumin, without causing brain edema or altering brain electrolytes, by perfusing 2.5 molal recrystallized D,L-lactamide into the internal carotid artery for 20--30 sec.
(6) Electrophoresis, microscopical observation and pH dependency of encapsulability of recrystallized cores revealed that the electrostatic attractive force between gelatin molecules in solution and a polymer attached to the core particle cause gelatin adsorption on the core surface to result in a great improvement in encapsulability.
(7) The porosity in lased enamel was overall very similar to that seen in enamel heated in an electric furnace to a temperature of 600 degrees C. The presence of recrystallized enamel crystals indicated a temperature rise of approximately 1000 degrees C and the grain growth indicated that a temperature > or = 1000 degrees C existed for some time after the laser irradiation.
(8) Products of cholesterol auto-oxidation were concentrated from several lots of USP-grade cholesterol by recrystallizing cholesterol from the methanol extract, retaining the mother liquor, and evaporating the residuum to dryness under vacuum.
(9) Recrystallization of the synthetic product produced material identical in all respects with natural PGE1.
(10) Experiments showed that sulphadimidine crystals recrystallized from ethanol had better dissolution and absorption characteristics than sulphadimidine crystals recrystallized from methanol.
(11) Thermal studies (DSC and HSM) showed that form II melts at 196 degrees C, while form I melts at 193 degrees C, immediately followed by a resolidification and remelt at 196 degrees C. The conversion of form II to form I was accomplished by recrystallization from ethanol or methanol, and the form I-to-form II transition was obtained by controlled heating of form I around 194 degrees C. Quantitative XRD was used to determine the polymorphic composition, with a detection limit of less than 1% of the minor form and a linearity of 0-10% form I in form II (correlation coefficient of 0.999).
(12) Microcapsule batch reproducibility depended mainly on the variation in particle size distribution of the recrystallized core material.
(13) After the administration of 3-H-testosterone to castrated hamsters, extracts from crude nuclei were separated into bound and free fractions by gel filtration of Sephadex G-25, Subsequent analysis by thin-layer chromatography and recrystallization showed that dihydrotestosterone was the predominant radioactive steroid in the bound fraction and that it increased steadily with time until 1 hr after the injection.
(14) 1,2-Dimethyl-3-hydroxy-4-pyridone (1), a crystalline oral iron chelator, forms an acetic acid solvate (2) on recrystallization from acetic acid and carbon tetrachloride.
(15) Recrystallization of the purified enzyme under different conditions readily produces a variety of single crystals.
(16) Ice recrystallization was practically absent at the temperature normally used in cryo-sectioning (-70 degrees C).
(17) A convenient, detailed procedure is given for the recrystallization of commercial dithionite from 0.1 M NaOH-methanol under anaerobic conditions.
(18) Thus, the protein penetrates partway into the bilayer, distorts the packing of the lipid fatty acid chains, and prevents recrystallization, thus decreasing the phase transition temperature.
(19) Winter stem fluid from the bittersweet nightshade, Solanum dulcamara L., also showed the recrystallization inhibition activity characteristic of the animal thermal hysteresis proteins (THPs), suggesting a possible function for the THPs in this freeze tolerant species.
(20) The chimeric proteins varied in their effectiveness of inhibiting ice recrystallization according to the number of 11-amino acid repeats present in the antifreeze moiety.