What's the difference between freeze and thaw?

Freeze


Definition:

  • (n.) A frieze.
  • (v. i.) To become congealed by cold; to be changed from a liquid to a solid state by the abstraction of heat; to be hardened into ice or a like solid body.
  • (v. i.) To become chilled with cold, or as with cold; to suffer loss of animation or life by lack of heat; as, the blood freezes in the veins.
  • (v. t.) To congeal; to harden into ice; to convert from a fluid to a solid form by cold, or abstraction of heat.
  • (v. t.) To cause loss of animation or life in, from lack of heat; to give the sensation of cold to; to chill.
  • (n.) The act of congealing, or the state of being congealed.

Example Sentences:

  • (1) In attacking the motion to freeze the licence fee during today's Parliamentary debate the culture secretary, Andy Burnham, criticised the Tory leader.
  • (2) Collagen production of rapidly thawed ligaments was studied by proline incubation at 1 day, 9 days, or 6 weeks after freezing and was compared with that of contralateral fresh controls.
  • (3) A simple method for ultrarapid freezing of cell cultures in monolayers was developed.
  • (4) We’re learning to store peak power in all kinds of ways: a California auction for new power supply was won by a company that uses extra solar energy to freeze ice, which then melts during the day to supply power.
  • (5) To selectively stain polyanionic macromolecules of growth plate cartilage and to prevent artifacts induced by aqueous fixation, proximal tibial growth plates were excised from rats, slam-frozen, and freeze-substituted in 100% methanol containing the cationic dye Alcian blue.
  • (6) The freeze-etch technique was used to study the morphology of Treponema refringens (Nichols).
  • (7) Reconstituted freeze dried allogeneic skin grafts contained virtually no blood, a phenomenon possibly analogous to the 'no reflow' phenomenon of microsurgery.
  • (8) The freezing procedure increased sperm motility in approximately 30% of samples from both animals.
  • (9) It is suggested that the intercalated disc functioned as a barrier to the freezing process.
  • (10) Healthbars such as Nakd fit this category and promise to deliver one of your five a day, based on the quantity of freeze-dried date paste used.
  • (11) Freeze-dried mannitol preparations were shown to be of a crystalline nature.
  • (12) Freezing may be valuable while quality control procedures are performed following radiolabeling as well as if temporary storage or shipment of radioantibodies prior to patient dosing is undertaken.
  • (13) The sea ice usually then begins to freeze again over the winter.
  • (14) We conclude that differences in incorporation between syngeneic and allogeneic bone grafts are reduced by pretreatment with deep-freezing or demineralisation.
  • (15) Previous studies are reviewed in the light of new information on retrograde axonal transport, circumventricular organs, the proper use of horseradish peroxidase, freeze-fracturing, immunocytochemistry and plasma protein gene expression in the developing human brain.
  • (16) Freezing enrichment cultures prior to testing for toxicity eliminated many nonbotulinal toxic substances that killed mice.
  • (17) The process of interaction between macrophages and promastigote and amastigote forms of Leishmania mexicana amazonensis was analyzed using freeze fracture and cytochemistry.
  • (18) The results of the rapid-freeze and deep-etch procedure showed that the ridges observed by the surface replica method consisted of linear arrangements of elliptical particles on the ES face of the plasma membrane.
  • (19) Cryotherapy with high-flow nitrous oxide was applied to the lid margin for 45 seconds in a freeze-thaw-freeze cycle.
  • (20) Three freeze-thaw cycles released a large proportion (50% to 60%) of the TCA-precipitable radioactivity from the worms.

Thaw


Definition:

  • (v. i.) To melt, dissolve, or become fluid; to soften; -- said of that which is frozen; as, the ice thaws.
  • (v. i.) To become so warm as to melt ice and snow; -- said in reference to the weather, and used impersonally.
  • (v. i.) Fig.: To grow gentle or genial.
  • (v. t.) To cause (frozen things, as earth, snow, ice) to melt, soften, or dissolve.
  • (n.) The melting of ice, snow, or other congealed matter; the resolution of ice, or the like, into the state of a fluid; liquefaction by heat of anything congealed by frost; also, a warmth of weather sufficient to melt that which is congealed.

Example Sentences:

  • (1) Collagen production of rapidly thawed ligaments was studied by proline incubation at 1 day, 9 days, or 6 weeks after freezing and was compared with that of contralateral fresh controls.
  • (2) Following thawing, the initial motility index (MI) scores of mf cryopreserved by either method were not significantly different from untreated controls; however, over a period of 15 days in culture the MI scores of both cryopreserved groups showed a small but significant overall decline, with the methanol technique producing the lowest scores.
  • (3) In order to maintain its activity, the enzyme was always stored in 1.0-ml aliquots at temperatures below -20 degrees C and each aliquot when thawed was used immediately; any left over enzyme was never reused.
  • (4) Cryotherapy with high-flow nitrous oxide was applied to the lid margin for 45 seconds in a freeze-thaw-freeze cycle.
  • (5) Three freeze-thaw cycles released a large proportion (50% to 60%) of the TCA-precipitable radioactivity from the worms.
  • (6) The effects of intravenous administration of DDAVP to blood donors and the use of DDAVP plasma for the production of cryoprecipitate in the closed thaw-siphon system were evaluated.
  • (7) Binding of [125I]-labelled ifenprodil, a non-competitive N-methyl-D-aspartate (NMDA) antagonist acting at the polyamine domain, was studied in washed, frozen-thawed synaptic membranes.
  • (8) These observations may be important in the development of laboratory protocols for freezing and clinical protocols for using frozen-thawed sperm.
  • (9) After being thawed at room temperature, the two CM samples were compared as to their pH, spinnbarkeit and ferning patterns, and it was found that they are quite similar.
  • (10) Freeze-thawed epimastigotes were used as a control antigen.
  • (11) To gain some understanding of the mechanism of cell fusion, cell ghosts prepared by freeze-thawing intact cells were incubated with intact cells.
  • (12) Particularly, the losses during blanching and thawing (drip) are discussed.
  • (13) However, mitochondrial susceptibility to rupture by freezing and thawing was not affected.
  • (14) Jackets were frozen for storage and were later thawed and placed on experimental alien lambs.
  • (15) This case illustrates: (1) acid medium, chymotrypsin, or sucrose are not needed for the procedure of zona cutting; (2) the zygotes resulting from zona cutting survive through freezing and thawing; and (3) oocyte retrieval can be done concomitant with conservative surgery for endometriosis.
  • (16) Ten eyes were treated with a single freeze-thaw cycle and were observed for 3 to 18 months.
  • (17) The choice of optimal freezing and thawing parameters is discussed.
  • (18) Although freezing and thawing produced additional decrements in all the assays, the hypotonic stress response was better by a factor of 3.5 than that previously obtained in a cryopreservation method using 0.5 molar glycerol.
  • (19) Lamb leg and rib roasts were more tender when cooked from the thawed state.
  • (20) Four- and eight-cell embryos from 3 mouse genotypes were cryopreserved to study the relationship of genetics and freezing variables on post-thaw survival.