(1) Synthetic DNA corresponding to the hydrophobic domain of cytochrome b5 was enzymatically fused in-frame to cloned DNA corresponding to the C-terminus of the Escherichia coli enzyme, beta-galactosidase.
(2) The latter findings reinforce the concept that in pathologic states associated with cerebral oedema, pinocytotic vesicles fuse to form transendothelial channels which transport plasma proteins into brain.
(3) DNA fragments coding for signal peptides with different lengths (28, 31, 33 and 41 amino acids from the translation initiator Met) were prepared and fused with the E. coli beta-lactamase structural gene.
(4) When these sequences were fused to the N terminus of yeast cytochrome oxidase subunit IV lacking its own presequence, they directed the attached subunit IV to its correct intramitochondrial location in vivo.
(5) In addition, 15 double mutant xylS genes were constructed in vitro by fusing parts of various mutant genes to produce mutant regulators exhibiting C-terminal and N-terminal amino acid substitutions.
(6) Descending neurons have opposite structural polarity, arising in the brain and terminating in segmental regions of the fused ventral ganglia.
(7) Some of them situated in a particular environment fused with the tail sequence to produce monomeric ubiquitin genes that were maintained across species.
(8) Fusing equimolecular amounts of 3-oxaspiro[5.5]undecane-2.4-dione with certain amino compounds afforded the corresponding N-substituted azaspirodiones.
(9) Theoretical analyses of parameters for submicron fluorescence recovery after photobleaching measurements in intact mitoplasts support the finding of highly mobile redox components diffusing at the same rates as determined in conventional fluorescence recovery after photobleaching measurements in fused, ultralarge inner membranes.
(10) A simple method has been developed for fusing synaptic vesicles into spherical structures 20-50 micron in diameter.
(11) The results obtained allow to conclude that heterophasic condition of the fused cells is one of the causes of pathological mitosis of polykaryons and of their death.
(12) At high protein concentrations, three footprints fuse to a 106-bp protected region, suggesting that this segment specifically binds several proteins of lower affinity or abundance.
(13) Traction spurs with segmental hypermobility were found more commonly at the L4-5 level in patients whose spines were not fused, particularly women.
(14) As for possible causes of reduced Leydig cell activity it was investigated whether the testis was (1) hypoplastic; (2) abnormally fused with the epididymis; (3) located in the abdomen; (4) or UT was associated with hypospadias.
(15) We report a case of seminoma associated with crossed fused renal ectopia and a duplicated vena cava.
(16) Large intracellular vacuoles, which arose from dilated cisternae of the rough-surfaced endoplasmic reticulum, were fused together, and marked swelling of the mitochondria was also noted.
(17) Apparently the myoblasts have become postmitotic and competent to fuse into muscle fibers during their initial exposure to fusion inducing medium, even though cytodifferentiation has been blocked.
(18) Our results clearly demonstrate that capillary GC analysis of amino acids using fused silica bonded-phase columns provides data with good precision and in general excellent agreement with ion-exchange analyses.
(19) We have perturbed the dynamics of the nuclear lamins by means of cell fusion between mitotic and interphase cells and have studied redistribution of lamins in fused cells as a function of extracellular pH levels.
(20) To identify regulatory elements of these promoters, we fused CSF-1R genomic sequences to bacterial reporter genes and introduced the resulting constructs into human cell lines and mouse fibroblasts.
Fusion
Definition:
(v. t.) The act or operation of melting or rendering fluid by heat; the act of melting together; as, the fusion of metals.
(v. t.) The state of being melted or dissolved by heat; a state of fluidity or flowing in consequence of heat; as, metals in fusion.
(v. t.) The union or blending together of things, as, melted together.
(v. t.) The union, or binding together, of adjacent parts or tissues.
Example Sentences:
(1) To identify the NHE-1 protein and to establish its cellular and subcellular localization in the rabbit kidney, we prepared antibodies to a NHE-1 fusion protein.
(2) Three criteria of fusion ventricular complexes were found to be undiagnostic for right and left ventricular complexes in SVE.
(3) Furthermore, these data support our previous suggestion that the expression of human lymphoid differentiation antigens in human-mouse lymphoid hybrids is influenced by the differentiation stage of the fusion partners.
(4) Several technical advantages of this method of fusion make this approach particularly useful in patients with rheumatoid arthritis.
(5) These differences point to the fact that the mechanisms that regulate satellite cell mitotic and fusion behavior are also not the same in all muscles.
(6) Expansion of the cell sheet following attachment, and the fusion of epiblasts advancing toward each other, does not require the presence of mineralocorticoid.
(7) The ophthalmic headache's crisis is caused, in fact, by a spasm of convergence on an unknown exophory of which the amplitude of fusion is satisfying, and the presence of which can only be seen with test under screen.
(8) In the companion paper, we quantitatively account for the observation that the ability of a solute to promote fusion depends on its permeability properties and the method of swelling.
(9) Opsin becomes incorporated into the disk membrane by a process of membrane expansion and fusion to form the flattened disks of the outer segment.
(10) One mutant, BS260, was completely noninvasive on HeLa cells and mapped to a region on the 220-kb virulence plasmid in which we had previously localized several avirulent temperature-regulated operon fusions (A.E.
(11) Using a soluble ICAM-2 Ig fusion protein (receptor globulin, Rg) we demonstrate the costimulatory effect of ICAM-2 during the activation of CD4+ T cells.
(12) With thermosensitive mutants non-defective for G and M antigens, cell fusion is much more extensive at the non-permissive temperature (39-6 degrees C) than at the permissive one (31 degrees C).
(13) This suggests that the fusion protein traps the SII in nonstimulatory interactions and that antibody 2-7B inhibits SII binding to RNA polymerase II.
(14) Ca2+ has a central role in various cellular phenomena involving membrane fusion.
(15) Polypeptides of egg-borne Sendai virus (egg Sendai), which is biologically active on the basis of criteria of the infectivity for L cells and of hemolytic and cell fusion activities, were compared by polyacrylamide gel electrophoresis with those of L cell-borne (L Sendai) and HeLa cell-borne Sendai (HeLa Sendai) viruses, which are judged biologically inactive by the above criteria.
(16) Pulse-chase experiments showed that the ornithine transcarbamylase precursor and the thiolase traveled from the cytosol to the mitochondria with half-lives of less than 5 min, whereas the three fusion proteins traveled with half-lives of 10-15 min.
(17) The results of this study suggest that the effects of benzylated CD4(81-92) derivatives on HIV-1 binding or fusion should not be used to reach conclusions about the function of the corresponding CD4 region.
(18) Construction of a repR-lacZ fusion proved that the increase in copy number was due to a proportional increase in the amount of RepR protein.
(19) The best understood fusion mechanism is that of influenza virus, for which sequences involved in pH-dependent fusion can be correlated with the crystallographic structure of the spike protein.
(20) The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form.