(1) HPLC was used to investigate this reaction and included the assignment of an HPLC peak to be due to N-OAc-GAF.
(2) The microsomal fraction catalyzed the binding of N-OH-GAF to DNA at a rate that was about twice that observed for N-OH-AAF.
(3) The level of Ca2+ in liver cytosol fraction in cortisone-treated mice was higher 2 hr after GAF injection than that in the cortisone alone-treated one.
(4) Endotoxin-tolerant mice were also nonresponsive to endotoxin-stimulated GAF production.
(5) Through the use of inhibitors of protein synthesis and inhibitors of protein kinases, the activation conditions of AAF, GAF, and ISGF-3 could be distinguished.
(6) Based on these assays, this represents a purification of GAF synthase of approximately 10,076- and 8925-fold with recoveries of 16% and 19%, respectively.
(7) This facile rearrangement reaction was studied for the case of N-acetoxy-N-glycolyl-2-aminofluorene (N-OAc-GAF), which gave N-hydroxy-N-(acetoxyacetyl)-2-aminofluorene (N-OH-AcAAF) as the sole product of this rearrangement.
(8) Patients were further randomized to receive on the first 4 postoperative days a) a standard dextrose-saline regimen (DS), b) an amino acid regimen (AA), or c) a glucose-amino acid-fat regimen (GAF) via a peripheral vein.
(9) GAF elutes from Sephadex G-200 along with markers of known molecular weight in the region of 100,000 to 200,000.
(10) after an intravenous injection of phenol-water lipopolysaccharide, but they react normally (produce GAF?)
(11) A five-day, double-blind parallel clinical study was conducted to determine the effect on plaque removal of a pre-brush rinse containing 0.03 triclosan (Irgacare MP, Ciba-Geigy Corp.) and 0.125% of a copolymer of methoxyethylene and maleic acid (Gantrez, GAF Corp.), as compared to a matching placebo pre-brush rinse.
(12) Covalent binding of N-OH-GAF to DNA was minimal through the action of cytosolic N,O-acyltransferase, which confirms our earlier observation that N-OH-GAF is a potent suicide inhibitor of this enzyme.
(13) It efficiently competed for the association of the GAF with the GBP promoter but this competition was due to the presence of two low affinity sites, the ISRE and an ISRE-like sequence, suggesting that the GAS and ISRE, though both having clear preferences for specific proteins, may nevertheless share a certain degree of structural homology.
(14) The liver glycogen level in 2 hr after injection with GAF plus cortisone-treated mice was markedly lower than that in cortisone alone-treated mice.
(15) The resistance inducing activity was retained after treatment of the extract with detergent (GAF Emulphogene BC 720) to remove lipopolysaccharide and adsorption to alum gel.
(16) Scott Walker says that women don’t really ever need abortions to save their lives , and Mike Huckabee – the gift who keeps on gaffing – is out there actually arguing that 10-year-old rape victims should be forced to give birth.
(17) With calmodulin and NADPH as cofactors, purified soluble GAF synthase induced an increase of 1.05 mumol of cGMP per 10(6) RFL-6 cells per 3 min per mg of protein.
(18) The activity of TO in cortisone treated mice after injection of purified GAF was markedly lower than that in cortisone alone treated mice.
(19) Interferon-gamma (IFN-gamma) induces the transcription of the gene encoding a guanylate binding protein by activating a latent cytoplasmic factor, GAF (gamma-activated factor).
(20) The AAF has characteristics very similar to those of the previously described GAF.
Gaffer
Definition:
(n.) An old fellow; an aged rustic.
(n.) A foreman or overseer of a gang of laborers.
Example Sentences:
(1) A breathless Sturridge was still trying to digest his part in the game when he paid tribute to Hodgson, saying: “I’m grateful to the gaffer for allowing me to score and it’s a beautiful feeling to represent your country in the rivalry against another great country.
(2) The gaffer’s not actually spoken to me and I’ll go in and say goodbye but I think it will be fine.
(3) I am grateful to the gaffer for the opportunity and God for allowing me to score.
(4) This one showed a girl bound with gaffer tape being forced to have sex with an adult.
(5) We look forward to it, the gaffer will look forward to it, he will have a game plan and will try to get into the next round.” It was Thistle’s first defeat in seven games but their defender Danny Seaborne said they had been undone by a striker enjoying a great run of form.
(6) The teams we’ve played against on the way to the final – Manchester United, which is always an important derby, Borussia Dortmund, because of the gaffer, and Villarreal, who are a very good side and fourth in La Liga – they are all worthy of playing in the Champions League.
(7) The manager and the chairman were a big influence; the gaffer made it clear last season that there was no way I was leaving and I didn’t want to.
(8) Under the gaffer we have controlled matches more than we've been controlled."
(9) Leicester let Premier League joy sink in before Champions League dreams begin Read more The striker said: “I think a good thing that helped us straight after that game was the gaffer had seen exactly how much we’d all fought for each other and put into that game, and because we weren’t in the week after because we’d been knocked out of the FA Cup he gave us a week off to completely forget about everything and recharge the batteries.
(10) The players deserve it, the gaffer and the staff deserve it and the fans deserve it.
(11) I am more comfortable playing up front but I will always do a job for the team, wherever the gaffer wants me to play.” Hurling remains a passion and although some of his Southampton colleagues have paid an interest, none is willing to pick up a stick and have a puck around.
(12) When his vehicle was recovered later, there was a roll of gaffer tape on his dashboard.
(13) I’ve had enough of all this,” which prompted the midfielder to round on his manager, replying: “You as well gaffer.
(14) The season is only midway through and the gaffer Ed Miliband and his chief tactician Cruddas are getting on pretty well.
(15) Gaffers seemed in short supply and none had, apparently, been allowed to use their own initiative in allowing who to release from the pen of police in which the protesters were corralled.
(16) "José's the man and it was hard for Davide playing in front of his old gaffer," he said.
(17) But me and the gaffer, we don’t make the decisions – there are people above us who make the decisions and they never approached me.” The manager himself had expressed public fear over the ability of Celtic to land transfer targets who would be suitable to ensure Champions League qualification once again.
(18) Anyway, do staff really vote on the basis of the views held by the gaffer?
(19) To experience this atmosphere and togetherness is an amazing feeling.” Asked whether he should be in the first XI against Slovakia on Monday, he said: “That’s the gaffer’s decision.
(20) The lighting assistants watch her while pretending to do something with gaffer tape; the catering team take their time gathering the coffee pots; the work experience girls simply try not to gawp.