(1) The rate-limiting step for O2, NO, and isonitrile binding to all five proteins is ligand migration up to the initial geminate state, and the rate of this process determines the overall bimolecular association rate constant for these ligands.
(2) Analysis of the effect of photoselection by the linearly polarized excitation pulse indicates that a major contribution to the apparent geminate rebinding in the 50-ns relaxation arises from rotational diffusion of molecules containing unphotolyzed heme-CO complexes.
(3) This is due to the influence of the fluorine substituent(s) on the basicity of the amine function proximal to the fluoromethylene group, this effect being amplified by geminal disubstitution.
(4) Significant subnanosecond geminate recombination is observed in oxyhemoglobin down to 150 K, while below 100 K this geminate recombination disappears.
(5) In addition, the first prevalence statistics for the bilateral occurrence of fusion and gemination in the primary and permanent dentitions are determined.
(6) We discuss geminate recombination measurements of cyanomet hybrid hemoglobins with NO and consider these results in terms of alpha and beta subunit heterogeneity.
(7) The results were analyzed quantitatively in terms of a three-step reaction scheme, MbX in equilibrium B in equilibrium C in equilibrium Mb + X, where Mb is myoglobin, B represents a geminate state in which the ligand is present in the distal pocket but not covalently bound to the iron atom, and C, a state in which the ligand is still embedded in the protein but further away from the heme group.
(8) The picosecond geminate rebinding of molecular oxygen was monitored in a variety of different human, reptilian, and fish hemoglobins.
(9) Comparing the kinetic and thermodynamic process of the O2 geminate reaction among several Mbs, we concluded that the geminate O2 reaction with Mb is governed by the dynamic motion of the protein which is sensitively controlled by the static interaction of the heme moiety with the surroundings.
(10) The geminal nature of the magnitude-COSY detected partners to the resolved C beta H peaks is confirmed by strong NOESY cross-peaks.
(11) Typical geminate virus particles were observed in extracts of plants infected with ORF AC3 mutants indicating that this gene is not essential for coat protein synthesis or virus assembly but possibly acts by modulating virus levels in infected tissues.
(12) However, the rates of geminate recombination of NO and O2 and the affinity of myoglobin for O2 were dependent upon the basicity of residue 45.
(13) The vicinal (vic) isomer was excreted in a 2 times higher amount (16 nmol) than the geminal (gem) isomer (8 nmol).
(14) The fraction geminate signal was least at delays where the maximum proportion of liganded T state tetramer is expected.
(15) Geminate recombination phases were observed at 30 ns and 1 microsecond following photodissociation.
(16) It is suggested that all succedaneous teeth that are joined or fused together by dentin be referred to as fused teeth because of the frequent difficulty in differentiating fusion and gemination in the adult dentition.
(17) The geminal beta-methylene protons for the two cysteines bound to the iron(II) center were clearly identified, as well as the C alpha H and one C beta H for each of the cysteines bound to the iron(III).
(18) Measurement of the geminate process in the infrared CO-stretch bands shows distributed activation enthalpies with different distributions for each band, transitions between two bands that correspond to photolyzed ligands, and kinetic hole burning.
(19) The kinetics of geminate recombination for the diliganded species alpha 2CO beta 2 and alpha 2 beta 2CO of human hemoglobin were studied using flash photolysis.
(20) Flash photolysis kinetics of carbon monoxide hemoglobin show a decrease in the fraction of ligand recombination occurring as geminate when the hemoglobin has fewer ligands bound.
Molecule
Definition:
(n.) One of the very small invisible particles of which all matter is supposed to consist.
(n.) The smallest part of any substance which possesses the characteristic properties and qualities of that substance, and which can exist alone in a free state.
(n.) A group of atoms so united and combined by chemical affinity that they form a complete, integrated whole, being the smallest portion of any particular compound that can exist in a free state; as, a molecule of water consists of two atoms of hydrogen and one of oxygen. Cf. Atom.
Example Sentences:
(1) A series of human cDNA clones of various sizes and relative localizations to the mRNA molecule were isolated by using the human p53-H14 (2.35-kilobase) cDNA probe which we previously cloned.
(2) Glucocorticoids have numerous effects some of which are permissive; steroids are thus important not only for what they do, but also for what they permit or enable other hormones and signal molecules to do.
(3) The results demonstrated that K2PtCl4 was bound to a greater degree than CDDP in this system with 3-5 and 1-2 platinum atoms respectively, bound per transferrin molecule.
(4) Complementarity determining regions (CDR) are conserved to different extents, with the first CDR region in all family members being among the most conserved segments of the molecule.
(5) Even with hepatic lipase, phospholipid hydrolysis could not deplete VLDL and IDL of sufficient phospholipid molecules to account for the loss of surface phospholipid that accompanies triacylglycerol hydrolysis and decreasing core volume as LDL is formed (or for conversion of HDL2 to HDL3).
(6) However, the presence of these two molecules was restored if testosterone was supplemented immediately after orchiectomy.
(7) In the second approach, attachment sites of DTPA groups were directed away from the active region of the molecule by having fragment E1,2 bound in complex, with its active sites protected during the derivatization.
(8) PMNs could be primed for PMA-triggered oxidative burst by muramyl peptide molecules (MDP) and two of its adjuvant active nonpyrogenic derivatives.
(9) These same molecules may be equally responsible for the pathologic characteristics of the immune response seen, for example, in inflammatory bowel diseases.
(10) A cDNA library prepared from human placenta has been screened for sequences coding for factor XIIIa, the enzymatically active subunit of the factor XIII complex that stabilizes blood clots through crosslinking of fibrin molecules.
(11) T cell costimulation by molecules on the antigen presenting cell (APC) is required for optimal T cell proliferation.
(12) The lipid A moiety was shown to be responsible for this novel biological activity of the LPS molecule.
(13) Both systems indicated that the Kupffer cell modified endotoxin by enriching the lipid content of the molecule and shortening the length of the O-antigen.
(14) Photoreactions induced in that proper sensitizer molecules absorb UV-light or visible light.
(15) At 100 microM-ACh the apparent open time became shorter probably due to channel blockade by ACh molecules.
(16) Flow cytofluorometric analysis of the strain distribution of the molecules defined by the mAb revealed that two of the antibodies (I-22 and III-5) were directed against nonpolymorphic determinants of Thy-1, whereas V-8 mAb reacted only with Thy-1.2+ lymphocytes.
(17) At a fixed concentration of nucleotide the effectiveness of elution was proportional to the charge on the eluting molecule.
(18) The relative rates of reduction of several spin-labeled molecules that partition differently across the hy-drophobic-interface of inner membranes from rat liver mitochondria were investigated.
(19) The seve polypeptide chains investigated had generalyy similar properties; all contained two residues per molecule of tryptophan and N-acetylserine was the common N-terminal amino acid residue.
(20) Much information has accumulated on the isolation and characterization of a heterogeneous group of molecules that inhibit one or more of the bioactivities of interleukin 1.