(1) Estimations of the degree of incorporation of 14C from the radioactive labeled carbohydrate into the glycerol and fatty acid moieties were carried out.
(2) Similar results were obtained with 1-oleoyl 2-acetylglycerol (OAG), whereas 1, 2 diolein, 1-oleoyl glycerol, or 4 alpha-phorbol 12, 13-didecanoate had no effect.
(3) Changes in renal renin levels after the administration of glycerol were not significant, although lower renal renin values were consistently found in rabbits with more severe impairment of renal function.
(4) When labelled long-chain fatty acids or glycerol were infused into the lactating goat, there was extensive transfer of radioactivity into milk in spite of the absence of net uptake of substrate by the mammary gland.
(5) sn-Glycerol 3-phosphate was found to decrease the quasi-stationary concentration of Fru 2,6-P2.
(6) Rhesus monkey BAT mitochondria (BATM) possess an uncoupling protein that is characteristic of BAT as evidenced by the binding of [3H]GDP, the inhibition by GDP of the high Cl- permeability or rapid alpha-glycerol-3-phosphate oxidation.
(7) Glucose formation from a range of substrates, with the exception of glycerol, was increased by an increase in extracellular Ca(2+) concentration.
(8) A search in protein data banks revealed that IclR has a score of similarity of 43.7% with GylR, a transcriptional regulator of the glycerol operon of Streptomyces coelicolor.
(9) We conclude, therefore, that a direct deacylation of the acyl groups at the primary alcohol level of the glycerol probably does not occur, but postulate that transacylations may occur to account for the removal of the acyl moiety.
(10) The dynamic properties of cross-bridge movement were investigated in glycerol-treated muscle fibers under various conditions by analyzing tension responses to two types of length change.
(11) A 4.1-kb EcoRI fragment which includes the gene (gldA) encoding a glycerol dehydrogenase (G1DH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from Bacillus stearothermophilus var.
(12) Methods are described for the analysis of glucose, lactate, pyruvate, alanine, glycerol, 3-hydroxybutyrate and acetoacetate in perchloric acid extracts of human blood, using the Cobas Bio centrifugal analyser fitted with a fluorimetric attachment.
(13) Glycerol permeation and thus its osmotic action may be less in the soleus than in other muscles.
(14) The activity of succinic dehydrogenase, a mitochondrial enzyme, was decreased by the deficiency, but the activities of fumarase, sn-glycerol-3-phosphate dehydrogenase, alcohol dehydrogenase, sn-glycerol-3-phosphate oxidase and fatty acid synthetase were unaffected.
(15) This oxidation is sensitive to catalase and glutathione plus glutathione peroxidase, suggesting a requirement for H2O2 in the overall pathway of glycerol oxidation.
(16) The defect is due to a single mutation in glycerol 3-phosphate acyltransferase (EC 2.3.1.15).
(17) Insertion of the fusion-generating phage Mud1 (Ap, lacZ) yielded two similar isolates, DC511 and DC512, which were unable to grow aerobically on acetate or alpha-ketoglutarate but which could use succinate, malate, fumarate, glycerol, and various sugars.
(18) Glycerol gradient centrifugation partially dissociated the complex to yield two peaks of exonuclease III activity, one at 7.7 S together with the DNA polymerase, and one at 4.0 S without polymerase activity.
(19) The native mass of factor a was estimated to be 240-260 kDa by gel filtration, but its sedimentation rate in a glycerol gradient was similar to that of a much smaller globular protein, suggesting an extended conformation.
(20) The radiochemical testings further indicate that the mutation has inactivated an inducible glycerol kinase, while a low residual activity may be due to a second, basal and non-inducible glycerol kinase, in accordance with a proposal by North (1973, 1974) that Neurospora has two glycerol kinases with these properties.
Glyoxal
Definition:
(n.) A white, amorphous, deliquescent powder, (CO.H)2, obtained by the partial oxidation of glycol. It is a double aldehyde, between glycol and oxalic acid.
Example Sentences:
(1) Therefore, all the results suggest that 1O2 formed from glyoxal is related to its mutagenesis, but that neither O2- nor H2O2 is intracellularly predominantly related to it.
(2) In addition, the possible mutagenic effect of glyoxal was assessed in postmeiotic cells up to 7 days after treatment.
(3) The complement-fixing activity of the virus was not reduced by methyl glyoxal treatment.
(4) The distribution of hydroxyproline oxidase, hydroxyoxoglutarate aldolase and alanine-glyoxalate transaminase were determined in detail.
(5) The location of calcium in a rapid-frozen and freeze-substituted maturation stage enamel organ of the rat incisors was demonstrated by means of the glyoxal bis(2-hydroxyanil) (GBHA) staining method, which formed insoluble red precipitates of calcium-GBHA complex.
(6) Based on the assumption that an inactivating particle keeps the channel closed, several protein-modifying agents including trypsin, papain, glyoxal, and phenylglycoxal that remove Na+-channel inactivation were tested.
(7) The extent of glyoxal reaction can be easily and sensitively measured using an assay based on the intercalation of ethidium into duplex DNA.
(8) The strains did not grow on oxalate, glyoxalate, glycolate, malonate or propionate.
(9) Condensation of the glyoxal obtained by cupric acetate oxidation of 21-hydroxycorticosteroids with acetous phenylhydrazine reagent affords a near UV chromophore.
(10) Three of the base replacements led to a more compact secondary structure of RNA segment 8, which seems to be responsible for the faster migration rate during PAGE and which seems to resist, at least partially, the treatment with glyoxal.
(11) By using a histofluorescent stain (glyoxalic acid) and a histochemical stain (thiocholine) in 17 fresh cadavres, we have demonstrated that the sympathetic fibres arise from sacral sympathetic ganglia.
(12) The chemicals studied were: bleomycin, t-butyl hydroperoxide, chromium trioxide, cumene hydroperoxide, formaldehyde, glyoxal, glutaraldehyde, hydrogen peroxide, paraquat, and phenylhydrazine.
(13) It was proved to be 8-(1'-D-ribityl)lumazine, which appeared to have been formed by a reaction between glyoxal and a possible intermediate in the cells.
(14) In this paper, both glyoxal bis(4-phenyl-3-thiosemicarbazone) (GBPT) and bathocuproine disulphonate (BCDS) methods for copper have been applied to the spectrophotometric determination of copper in foods, feedingstuffs and plants.
(15) MGR I was specific to 2-oxoaldehydes [glyoxal, methylglyoxal (Km = 15.4 mM) and phenylglyoxal], whereas MGR II was active on both 2-oxoaldehydes [glyoxal (Km = 10 mM), methylglyoxal (Km = 1.43 mM), phenylglyoxal (Km = 4.35 mM) and 4,5-dioxovalerate] and some aldehydes (propionaldehyde and acetaldehyde).
(16) The glyoxal bis(guanylhydrazone) chain of the EMGBG cation deviated strongly from planarity, thus differing dramatically from the corresponding chains of the glyoxal, methylglyoxal and propylglyoxal analogs.
(17) In contrast to unmodified tRNA, glyoxalated tRNA was rapidly degraded upon injection.
(18) Glyoxal treatment significantly increased the incidence of adenocarcinomas in the pylorus of the glandular stomach of rats pretreated with MNNG and sodium chloride.
(19) Total cytoplasmic or nuclear polyadenylated RNAs from infected cells were denatured with glyoxal, separated by electrophoresis on agarose gels, and transferred to diazobenzyloxymethyl paper.
(20) With a single oral high-dose treatment of glyoxal, a great decline in the incorporation of L-[3H]leucine was shown particularly in the liver, and this probably led in part to a reduction in the serum protein levels in rats following subchronic exposure to glyoxal.