(1) Positive HEP values were obtained in 40 patients out of 42 with malignant tumours and in 5 patients out of 12 with other kidney diseases.
(2) Extracts were also prepared from vaccinia-infected HEp-2, RK and W-K cells respectively.
(3) One peptide, designated Hep III, which is thirteen amino acids in length and binds heparin, was active in directly promoting keratinocyte adhesion.
(4) An association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per HEp-2 cell in the adhesion assay.
(5) The secretion kinetics of nine proteins by Hep G2 cells in culture was investigated using pulse-chase techniques and immunoisolation of proteins with monospecific antibodies.
(6) This finding appeared to be due to Hep G2 cells expressing lipase activities which led to triacylglycerol and phospholipid hydrolysis and lipid reuptake.
(7) DT diaphorase, purified to homogeneity from human Hep G2 cells, did metabolize CB 1954 to this 4-hydroxylamino product, but the rate of CB 1954 reduction and thus production of the cytotoxic product, was much lower than that of purified Walker enzyme (ratio of Kcat = 6.4).
(8) By electron microscopy, E2348 was seen to adhere to HEp-2 cells in a manner that closely resembled EPEC adhesion to intestinal mucosa; bacteria were intimately attached to projections of the apical HEp-2 cell membrane and caused localized destruction of microvilli.
(9) These examinations showed that only the freshly isolated strain of Aa was found within the HEp-2 cells.
(10) B-PEO-HEP coated grafts still showed patency after 3 days.
(11) For poliovirus types 2 and 3 the observed differences in titres were highly significant (p = 0.001) between Hep 2C and Vero cells, Hep 2C and MRC-5 and also between Vero and MRC-5 cells, with higher titres on Hep 2C cells.
(12) R325-beta TK+, a herpes simplex virus 1 mutant carrying a 500-base-pair deletion in the alpha 22 gene and the wild-type (beta) thymidine kinase (TK) gene, was previously shown to grow efficiently in HEp-2 and Vero cell lines.
(13) Methods are described that are used for the titration of antinuclear, anticentromere, and anti-Scl-70 antibodies in systemic scleroderma, systemic lupus erythematosus, and rheumatoid arthritis: indirect immunofluorescence with various antigenic substrates (sections of fresh-frozen rat liver and Hep-2 cell culture), counter-current immunoelectrophoresis, isolation of Scl-70 antigen.
(14) The O157:H8 strains did not produce VT. All gave localised attachment to HEp-2 cells, associated with a positive fluorescence-actin staining test, and all hybridised with the E coli attaching and effacing (eae) probe.
(15) Hep G2 cells produce surplus A alpha and gamma fibrinogen chains.
(16) Adhesion may be studied in vitro systems using HeLa or HEp-2 cells, to which EPEC adhere in a localized pattern.
(17) The serum inhibitory effect appears limited to primary cells because no difference in Ad41 replication, as assayed by accumulation of Ad41 DNA, was found in infected continuous cell lines (HEp-2, 293) cultivated p.i.
(18) Additionally, the invasiveness of C. jejuni M96, a clinical isolate, was significantly increased for HEp-2 cells preinfected with coxsackievirus B3.
(19) Hep 5 impeded binding and degradation of 125I-labelled bovine LPL by perfused rat livers.
(20) To evaluate this possibility, three human cell lines, Hep G2, Hep 3B, and HA 22T, all thought to be arrested in different stages of differentiation based on their biochemical and morphological characteristics, were used as models.
Restricted
Definition:
(imp. & p. p.) of Restrict
Example Sentences:
(1) However, medicines have an important part to play, and it is now generally agreed that for the very poor populations medicines should be restricted to those on an 'essential drugs list' and should be made available as cheaply as possible.
(2) These eight large plasmids had indistinguishable EcoRI restriction patterns.
(3) The findings clearly reveal that only the Sertoli-Sertoli junctional site forms a restrictive barrier.
(4) Four other independent LCMV-GP2(275-289) specific H-2Db-restricted CTL clones also expressed V alpha 4 and V beta 10 gene elements.
(5) This analysis demonstrated that more than 75% of cosmids containing a rare restriction site also contained a second rare restriction site, suggesting a high degree of CpG-rich restriction site clustering.
(6) In order to determine the extent of this similarity, I have developed a panel of probes for many of the Pacl restriction fragments and have shown that most of the Pacl and Notl fragments found in MBa are also present in MBb.
(7) In both experiments, Gallus males were placed on a commercial feed restriction program in which measured amounts of feed are delivered on alternate days beginning at 4 weeks of age.
(8) the class- and specificity-restricted antigen-sensitive units.
(9) Possibilities to achieve this both in the curative and the preventive field are restricted mainly due to the insufficient knowledge of their etiopathogenesis.
(10) A sperm whale myoglobin gene containing multiple unique restriction sites has been constructed in pUC 18 by sequential assembly of chemically synthesized oligonucleotide fragments.
(11) Northern hybridization analysis of R. toruloides RNA with a restriction fragment encoding part of the PAL gene indicates that PAL mRNA is 2.5 kilobases in length.
(12) Dietary factors affect intestinal P450s markedly--iron restriction rapidly decreased intestinal P450 to beneath detectable values; selenium deficiency acted similarly but was less effective; Brussels sprouts increased intestinal AHH activity 9.8-fold, ECOD activity 3.2-fold, and P450 1.9-fold; fried meat and dietary fat significantly increased intestinal EROD activity; a vitamin A-deficient diet increased, and a vitamin A-rich diet decreased intestinal P450 activities; and excess cholesterol in the diet increased intestinal P450 activity.
(13) Unilateral VNAB lesions induced similar alterations but these were restricted to the ipsilateral PVN and median eminence.
(14) In contrast, in primordial follicles, FSH was restricted to the germ cell but was present in both the oocyte cytoplasm and germinal vesicle.
(15) It delimitates the restrictive conditions in which such methods could be used for clinical but not research purposes.
(16) We propose that the results mainly reflect a variable local impact of infection control and that a much more restrictive use of IUTCs is possible in many wards.
(17) Restriction fragment length polymorphisms (RFLPs) were studied in a large Algerian family which includes 6 haemophiliacs and a previously described case of female haemophilia A.
(18) This suggested that carcinogen-induced error incorporation during DNA synthesis was restricted solely to the treatment of a deoxynucleotide template.
(19) The UNTR rats were subjected to a continuous food restriction to maintain body weights equal to those of the TR rats.
(20) Male Sprague Dawley rats either trained (T, N = 9) for 11 wk on a rodent treadmill, remained sedentary, and were fed ad libitum (S, N = 8) or remained sedentary and were food restricted (pair fed, PF, N = 8) so that final body weights were similar to T. After training, T had significantly higher red gastrocnemius muscle citrate synthase activity compared with S and PF.