(1) Zebrin II-negative Purkinje cells are present in a continuous region encompassing the rostromedial part of the valvula, the lobus transitorius, lobe C1 and the ventral part of lobe C2, and in a small, lateral zone of the posterior part of the caudal lobe.
(2) The distribution of mossy fiber terminals originating from the lower thoracic-higher lumbar spinal cord was compared to the distribution of zebrin I bands.
(3) In each hemicerebellum there is one zebrin II+ band abutting the midline (P1+), and two others laterally in the vermis (P2+, P3+).
(4) A fourth zebrin II+ compartment straddles the paravermian region (P4+).
(5) A monoclonal antibody to zebrin II from the weakly electric fish Apteronotus recognizes a 36 kD polypeptide in homogenates of Monodelphis cerebellum that appears to be identical to the antigen in the rat.
(6) In the adult gymnotiform teleost Eigenmannia, Purkinje cells in the corpus cerebelli (CCb), lateral valvula cerebelli (VCbl), and eminentia granularis anterior (EGa) are zebrin II+.
(7) One such marker is the polypeptide antigen zebrin I that is recognized by monoclonal antibody (mab) Q113.
(8) In order to establish whether the different compartments share a common organizational plan, a systematic comparative analysis of the patterns of parasagittal zonation in the cerebellar cortex of the rat has been undertaken, by using the parasagittal compartmentation of zebrin I+ and zebrin I- Purkinje cells as revealed by monoclonal antibody Q113 as a reference frame.
(9) One such marker is the antigen zebrin I, a 120 kD polypeptide of unknown function that is expressed differentially by a subset of Purkinje cells.
(10) Zebrin II antigenicity is first present at 6 days postspawning (P6) in the EGa and at P8 in the CCb.
(11) In the present study, zebrin I has been used to reveal the molecular heterogeneity of the cerebellar cortex in the squirrel monkey (Saimiri sciureus).
(12) Zebrin I positive Purkinje cells appeared in seven sagittal bands (P1+ to P7+ bands).
(13) Immunocytochemistry of apteronotid brains reveals that zebrin II immunoreactivity is confined exclusively to Purkinje cells in the corpus cerebelli, lateral valvula cerebelli, and the eminentia granularis anterior.
(14) Additionally, Eigenmannia has a third class of Purkinje cells, in the EGp and EGm, that never express zebrin II immunoreactivity, indicating that zebrin II expression is not an obligatory feature of Purkinje cell development in all vertebrates.
(15) In weakly electric gymnotiform teleosts, monoclonal antibody anti-zebrin II recognizes developing pyramidal cells in the ampullary organ-receptive medial segment of the medullary electrosensory lateral line lobe (ELL) and in the mechanoreceptive nucleus medialis.
(16) During postnatal development, zebrin II is first expressed between P14 and P21 in Purkinje cells of the posterior lobe vermis, and spreads throughout the cerebellar cortex by P28.
(17) In all there are 7 zebrin II+ and 7 zebrin II- compartments in each hemicerebellum.
(18) MabQ113 recognizes a polypeptide antigen, zebrin I, that is confined to a subset of Purkinje cells.
(19) Purkinje cells in the eminentia granularis posterior (EGp) and medialis (EGm) and the medial valvula cerebelli (VCbm) are zebrin II-.
(20) Examination of these cells with the zebrin antibodies demonstrates that in spite of the morphologic and laminar disorganization of these cells in the anterior lobe, they are organized into the appropriate number of correctly positioned immunopositive zebrin clusters.