(1) Anti-synthetic HIRP(957-980) serum HIR-27 was proved to cross-react with HIRP-related proteins in solubilized human placental membranes.
(2) We have investigated insulin responsiveness in relation to insulin sensitivity during sequential hyperglycemic clamping in low insulin responders (LIR), high insulin responders (HIR) and in women with a history of gestational diabetes (GD).
(3) 80% of internalized receptors after 20 min), the internalization in HIR-B cells reaches a maximum (approx.
(4) Human insulin receptor substrate-1 (hIRS-1) cDNAs were cloned from a lambda GT11 expression library using a monoclonal antibody (MAb) produced against a human hepatocellular carcinoma (HCC) cell line (FOCUS).
(5) Within 3 h after exposure to 70 nM insulin, ODC enzyme activity increased approximately 50-fold and mRNA accumulation 3-fold in the HIR 3.5 cells but not in normal fibroblasts.
(6) To further explore the specificity of the IR tyrosine phosphokinase (TPK) domain in IR function, we have altered the human IR (hIR) cDNA to encode truncated insulin-independent TPKs, which are expressed in chinese hamster ovary (CHO) cells as either membrane-anchored or cytosolic proteins.
(7) Glucose disposal and whole body carbohydrate oxidation were markedly lower in NIR and HIR vs. NIS (P less than 0.001 for disposal and oxidation).
(8) Internalization, degradation, and insulin-induced down-regulation of insulin receptors were studied comparatively in transformed Chinese hamster ovary (CHO) cell lines, CHO.T and CHO.IR.ros, respectively expressing either the wild-type human insulin receptor (hIR) or a mutated hybrid receptor in which the transmembrane and cytoplasmic domains of hIR were replaced by corresponding domains of the transforming protein p68gag-ros (v-ros) of avian sarcoma virus UR2.
(9) In conclusion, the HIR delta CT receptor retains intact protein kinase activity in vitro.
(10) Cells expressing 6.4 X 10(3) and 1.25 X 10(6) normal receptors and 2.5 X 10(5) HIR delta CT receptors, as well as control Rat 1 fibroblasts were selected for further analysis.
(11) Highly intensive physical loading suppresses the development of DH and enhances the suppressive effect of staphylococci on SRBC-induced HIR.
(12) Our experiments with the hIR protein have been designed to address a very general question of transmembrane receptor structure and function: What are the roles and interactions of the various deduced structural domains of such molecules in the initiation of the response of cells to extracellular signals?
(13) Mono- and polyauxotrophic mutant of I, II and R-phases by hir, ilv, pro, and mtl markers were obtained by treatment of Sh.
(14) Physical loading of low intensity normalizes staphylococcus-suppressed HIR to SRBC, but produces no effect on staphylococcus-induced DH.
(15) Transfection of normal HIR cDNA produced normal insulin receptors on the plasma membranes in COS 7 cells.
(16) Incubation for 18 hrs with 1 microM insulin resulted in a similar decrease (to approximately 35% of control) of all the hIR mRNA species.
(17) Seven regions of the alpha subunit of human insulin receptor (HIR) were synthesized and examined for their ability to bind radioiodinated insulin.
(18) This cDNA (HIR delta CT), as well as cDNA encoding the complete receptor (HIRc) was transfected into Rat 1 fibroblasts.
(19) To this end the following receptor model system consisting of two receptors was co-expressed in NIH 3T3 cells: a kinase inactive human insulin receptor (HIR K1018A) and a chimeric (EIR) receptor corresponding to the extracellular and transmembrane domains of the human EGF receptor and the cytosolic domain of the human insulin receptor beta subunit.
(20) The resulting virus, named UIR, contains the hIR sequence fused to the 5' portion of the UR2 gag gene coding for p19.