(1) The extents of phospholipid hydrolysis were relatively low in brain homogenates, synaptic plasma membranes and heart ventricular muscle.
(2) The constitution of chromosomes in the two plasmacytomas remained remarkably stable in their homogeneous modal population.
(3) Gel filtration of the 40,000 rpm supernatant fraction of a homogenate of rat cerebral cortex on a Sepharose 6B column yielded two fractions: fraction II with the "Ca(2+) plus Mg(2+)-dependent" phosphodiesterase activity and fraction III containing its modulator.
(4) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
(5) Concentrations of alpha-lactalbumin and alpha s1-casein were measured in media and explant homogenates by radioimmunoassay, as an index of lactogenesis.
(6) Further subfractionation disclosed that the acetyltransferase activity was most enriched in the Golgi fraction, in which its specific activity was some ninefold greater than in the total homogenate.
(7) Gamma-irradiated splenic homogenates of armadillos infected with M. leprae proved sterile by conventional tests and media.
(8) A technique, using Nuclepore polycarbonate membrane filters as a containing medium for very small volumes of ionic standard solutions, to produce homogeneous ice standards is described.
(9) In vitro studies showed that BOF-A2 was rapidly degraded to EM-FU and CNDP in homogenates of the liver and small intestine of mice and rats, and in sera of mice, rats and human, and the conversion of EM-FU to 5-FU occurred only in the microsomal fraction of rat liver in the presence of NADPH.
(10) Plasma membranes were obtained from a homogeneous population of rabbit red blood cells at different maturation periods.
(11) The effect of diethylstilbestrol (DES) on the percent conversion of a 14C-progesterone (14C-P) substrate to 14C-testosterone (14C-T) when added to incubates fo rat testicular homogenates has been measured.
(12) While the reduced form of the "derived" polyphenolic compounds, generated during tissue homogenization, appeared to enhance dye binding with bovine serum albumin, their influence on the protein assay directly in crude homogenates was extremely diverse.
(13) Enzyme prepared in this manner was homogeneous according to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels and immunoelectrophoresis using antiserum directed against it.
(14) In voltage-clamp experiments the ion current flowing through the channels was homogeneous indicating a defined conformation and a uniform size.
(15) The inhibitory effect was not due to contaminating rT3 in the liver homogenates.
(16) The final preparation was homogeneous and a single polypeptide of 18,000 daltons as judged by SDS-polyacrylamide gel electrophoresis.
(17) This enzyme was purified to homogeneity and exhibited an apparent molecular weight of 36,000 on sodium dodecyl sulfate gels and 180,000 on a TSK G-3000SW column in the presence of Triton X-100.
(18) Computed tomography (CT) is the most sensitive radiologic study for detecting these tumors, which usually are small, round, sharply marginated, and of homogeneous soft tissue density.
(19) Thus, whereas CD3-associated molecules isolated from polyclonal CD3+WT31+ populations (expanded in IL 2 under the same culture conditions) appeared as diffuse bands, CD3-associated molecules isolated from CD3+WT31- populations displayed a homogeneous molecular mass.
(20) Although this operational classification does not produce etiologically homogeneous groups, it is believed to have pragmatic utility with respect to planning targeted surveillance and management strategies.
Purification
Definition:
(n.) The act of purifying; the act or operation of separating and removing from anything that which is impure or noxious, or heterogeneous or foreign to it; as, the purification of liquors, or of metals.
(n.) The act or operation of cleansing ceremonially, by removing any pollution or defilement.
(n.) A cleansing from guilt or the pollution of sin; the extinction of sinful desires, appetites, and inclinations.
Example Sentences:
(1) During enzyme purification two nucleases were identified.
(2) Change of steps in achieved just by varying the reaction conditions without any product purification.
(3) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
(4) Stable factor-dependent B-cell hybridomas were used to monitor the purification of the growth factor from the supernatant of a clonotypically stimulated mouse helper T-cell clone.
(5) After immunoadsorbent purification, the final step in a purification procedure similar to that adopted for colon cancer CEA, two main molecular species were identified: 1) Material identical with colon cancer CEA with respect to molecular size, PCA solubility, ability to bind to Con A, and most important the ability to bind to specific monkey anti-CEA serum.
(6) Currently there are no IOC approved definitive tests for these hormones but highly specific immunoassays combined with suitable purification techniques may be sufficient to warrant IOC approval.
(7) A rapid method is described for the purification and analysis of synthetic oligonucleotides, based on reversed-phase high-performance liquid chromatography.
(8) The influence of exogenous mitogens (RFG, TGF beta 1 and insulin) and autocrine factor (at different step of purification) on the growth of Morris hepatoma 7777 (MH) cells was estimated by both methods.
(9) In a previous publication the purification and properties of two protein kinases (KI and KII) from a soluble fraction of bovine corpus luteum and the stimulation of the latter fol.
(10) for 48 h followed by Leydig cell isolation and purification resulted in a decrease in the maxima of hCG-induced cAMP accumulation and testosterone production by approximately 70% and approximately 55%, respectively, when compared to cells of control mice.
(11) These plasmids allow expression of native or truncated forms of the enzyme and easy purification of the products.
(12) The enzyme extracted from strains containing the recombinant plasmid was identical to N. crassa catabolic dehydroquinase by the criteria of heat stability, ammonium sulfate fractionation, immunological crossreactivity, molecular weight, and purification characteristics.
(13) Three triacetinases (A, B and C) were shown to undergo reciprocal conversions under storage and during some purification procedures (effect of pH, ionic strength, ion-exchange chromatography, concentration, lyophilization, etc.).
(14) Sindbis virus nucleocapsids were isolated from mature virions by a two-step purification method.
(15) The major scrapie prion protein, designated PrP 27-30, exhibited both charge and size heterogeneity after purification from infected hamster brains.
(16) We have used this procedure successfully during the purification of epidermal glycoproteins.
(17) The purification and concentration of these viruses in their monomeric forms is hazardous when conventional "tube" rotors are used since they invariably result in dissociation and aggregation of the virus particles.
(18) These methods can be applied to the purification and characterization of the as yet undefined secretory and circulating forms of PTHrP.
(19) The possibility that mammalian DNA topoisomerase II is an intracellular target which mediates drug-induced DNA breaks is supported by the following studies using 4'-(9-acridinylamino)methane-sulfon-m-anisidide (m-AMSA): (a) a single m-AMSA-dependent DNA cleavage activity copurified with calf thymus DNA topoisomerase II activity at all chromatographic steps of the enzyme purification; (b) m-AMSA-induced DNA cleavage by this purified activity resulted in the covalent attachment of protein to the 5'-ends of the DNA via a tyrosyl phosphate bond.
(20) These experiments may provide the basis for the expanded use of immobilized lectins for purification and characterization of hydrolases and other glycoproteins.