What's the difference between human and protoplast?

Human


Definition:

  • (a.) Belonging to man or mankind; having the qualities or attributes of a man; of or pertaining to man or to the race of man; as, a human voice; human shape; human nature; human sacrifices.
  • (n.) A human being.

Example Sentences:

  • (1) The absolute recoveries of diazepam, nordazepam and flurazepam in human milk were 84, 86 and 92% and in human plasma 97, 89 and 94%, respectively.
  • (2) Stimulation of human leukocytes with various chemical mediators such as TPA, f-Met-Leu-Phe, LTB4, etc.
  • (3) It was tested for recovery and separation from other selenium moieties present in urine using both in vivo-labeled rat urine and human urine spiked with unlabeled TMSe.
  • (4) The distribution and configuration of the experimental ruptures were similar to those usually noted as complications of human myocardial infarction.
  • (5) By electrophoresis and scanning densitometry, actin was found to constitute about 4% to 6% of the total cellular protein in the human corneal epithelium.
  • (6) A series of human cDNA clones of various sizes and relative localizations to the mRNA molecule were isolated by using the human p53-H14 (2.35-kilobase) cDNA probe which we previously cloned.
  • (7) Assessment of the likelihood of replication in humans has included in vitro exposure of human cells to the potential pesticidal agent.
  • (8) Herpesviruses such as EBV, HSV, and human herpes virus-6 (HHV-6) have a marked tropism for cells of the immune system and therefore infection by these viruses may result in alterations of immune functions, leading at times to a state of immunosuppression.
  • (9) After stimulation with lipopolysaccharide and calcium ionophore A23187, culture supernatants of clones c18A and c29A showed cytotoxic activity against human melanoma A375 Met-Mix and other cell lines which were resistant to the tumor necrosis factor, lymphotoxin and interleukin 1.
  • (10) Phospholipid methylation in human EGMs is distinctly different from that in rat EGMs (Hirata and Axelrod 1980) in that the human activity is not Mg++-dependent, and apparent methyltransferase I activity is located in the external membrane surface.
  • (11) This bone could not be degraded by human monocytes in vitro as well as control bone (only 54% of control; P less than 0.003).
  • (12) On the other hand, human IL-9, which is a homologue to murine P40, was cloned from a cDNA library prepared with mRNA isolated from PHA-induced T-cell line (C5MJ2).
  • (13) These results suggest the presence of a new antigen-antibody system for another human type C retrovirus related antigens(s) and a participation of retrovirus in autoimmune diseases.
  • (14) The promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin VH gene and the LTR of the human T-lymphotropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected human T cell line producing viral proteins).
  • (15) Detergent-solubilized HLA antigens were isolated from a human lymphoblastoid cell using an anti-beta2-microglobulin immunoaffinity column.
  • (16) We postulate that FAA may affect the human peripheral and mucosal immune system.
  • (17) The human placental villus tissue contains opioid receptors and peptides.
  • (18) The origins of aging of higher forms of life, particularly humans, is presented as the consequence of an evolved balance between 4 specific kinds of dysfunction-producing events and 4 kinds of evolved counteracting effects in long-lived forms.
  • (19) The result has been called the biggest human upheaval since the Second World War.
  • (20) It was the purpose of the present study to describe the normal pattern of the growth sites of the nasal septum according to age and sex by histological and microradiographical examination of human autopsy material.

Protoplast


Definition:

  • (n.) The thing first formed; that of which there are subsequent copies or reproductions; the original.
  • (n.) A first-formed organized body; the first individual, or pair of individuals, of a species.

Example Sentences:

  • (1) 25% of the incorporated radioactivity in protoplast lysates and approx.
  • (2) We have isolated an auxin-regulated cDNA, parB, from the early stage of cultured tobacco mesophyll protoplasts.
  • (3) Several subcellular fractions were derived from OK-432 and only the cytoplasmic and protoplast membrane fractions showed cytotoxic activity against the OK-432-sensitive tumor cell lines, although the cytotoxicity obtained was greatly less than the whole microorganism OK-432.
  • (4) The pH activity profile, cofactor requirements, and kinetic parameters of the endogenously activated chitin synthase were identical to those of the trypsin-activated enzyme in protoplast membranes.
  • (5) Osmotic gradient across the membrane of nonsonicated liposomes and rose petal protoplasts are shown to induce swelling.
  • (6) Unlike malate oxidation by osmotically shocked protoplasts, endogenous protoplast repiration was resistant to ferricianide 5.10(-4) M).
  • (7) The inhibition was also observed when cells were incubated with mercaptoethanol or when mercaptoethanol and DTT were used to prepare protoplasts.
  • (8) Regeneration of cells from protoplasts was monitored throughout the growth cycle and was most efficient when cells of either S. fradiae or S. griseofuscus were taken from the transition phase between the exponential and stationary growth phases.
  • (9) (2) Protoplast fusion analysis indicates that rosamicin-nonproducing characteristics of MR 217-AF2 and MR 217-AF3 strains induced by the acriflavine treatment is due to chromosomal mutation or rearrangement but not to loss of a plasmid.
  • (10) When electroporated into maize protoplasts from a suspension cell line not synthesizing anthocyanins, reporter genes with Bz2, Bz1, and A1 promoters are expressed only when both R and C1 expression plasmids are co-electroporated.
  • (11) In the presence of a suitable carbon source, whole cells and protoplasts of Saccharomyces cerevisiae synthesized glycerol as a compatible organic solute in response to increased external osmotic pressure.
  • (12) Tobacco protoplasts were transformed with an expression construct containing a translational fusion between mature alpha-amylase from Bacillus licheniformis and the signal peptide of the tobacco PR-S protein.
  • (13) The number of binding sites on bacilli and protoplasts is determined for each phage.
  • (14) In both the experiments there were detected cells in their majority with thinner walls, L-form-like structures, protoplasts and single conglomerates of the cells with thicker walls and anomalous division and the cells at the moment of lysis.
  • (15) Rate determinations were made with both, intact cells and with preparations containing secreted enzymes from protoplasts.
  • (16) The effects of Mg2+ and Ca2+ ions on the efficiency of the plasmid transformation of lysozyme-treated Streptococcus lactis protoplasts were compared.
  • (17) Tnt1 has been isolated after its transposition into the nitrate reductase (NR) structural gene of tobacco, and transposition events have been detected through in vitro selection of spontaneous NR-deficient (NR-) mutant lines in cell cultures derived from tobacco mesophyll protoplasts.
  • (18) The effect of treatment of the protoplasts and cell membranes of C. albicans with polyenic entibiotics on formation of the ribosomal-membrane complex was studied in vitro.
  • (19) On the other hand, R medium without MgCl2 but with penicillin G supported development of L-form type colonies at high rate (13-15%) from the inoculated protoplasts.
  • (20) In comparison, in protoplasts a region upstream of the AC2 open reading frame was shown to have moderate promoter activity.