(1) Furthermore, it is insufficient to fully account for the transmembrane chemical shift differences observed for dimethyl methylphosphonate and hypophosphite.
(2) The formate analogue hypophosphite has been characterized as a specific kcat inhibitor of pyruvate formate-lyase which destroys the enzyme radical.
(3) Adaptation to hypophosphite, however, led simultaneously to phosphite adaptation, so that these cells can utilize both P-compounds as a substitute for phosphate.
(4) The difference in the magnetic susceptibility of the intra- and extracellular compartments contributes to the observed separation of the intra- and extracellular resonances of dimethyl methylphosphonate and hypophosphite.
(5) Trimethyl phosphate, dimethyl methylphosphonate, diethyl methylphosphonate, trimethylphosphine oxide, and the hypophosphite, phenylphosphinate, and diphenylphosphinate ions all contain the phosphoryl functional group.
(6) The observed adaptation pattern, reflected by the alterations of phosphatase activity, was qualitatively equal with PO3-3 and PO3-2, but quantitatively different, because the response to hypophosphite gave much higher values than the increase obtained with phosphite.
(7) Zaprionus paravittiger fed with an antioxidant (sodium hypophosphite, 1 X 10(3) microM) supplemented diet exhibited adaptive compensatory responses in catalase activity (quantitative as well as qualitative).
(8) The intracellular NMR-determined viscosities from red cells, ranging in volume from 65.5 to 100.1 fl, varied from 2.10 to 2.67 mPa s. This is consistent with the translational diffusion coefficients of the hypophosphite ion altering by only 20%, whereas the values determined from bulk viscosity measurements conducted on lysates of these cells are consistent with a 230% change.
(9) It was also inhibited by hypophosphite, an inhibition that was reversed by formate.
(10) Formaldehyde, hypophosphite, nitrate, and bicarbonate all inhibited the oxidation of formate.
(11) Sodium hypophosphite (1 x 10(3) microM) supplementation in the diet of Zaprionus paravittiger resulted in adaptive responses in the quantitative as well as qualitative activity of peroxidase.
(12) These compounds include phosphate, phosphite, hypophosphite, fluorophosphate, thiophosphate, methylphosphonate, and dimethylphosphinate.
(13) On the basis of our findings, the previous work of Knappe and co-workers, the likelihood that hypophosphite is a formate analogue, the known susceptibility of both hypophosphite and formate to homolysis, and a chemical precedent for homolytic cleavage of pyruvate, we offer a preliminary mechanistic proposal for the lyase reaction.
(14) Hypophosphorus acid has a single pKa of 1.1 and at physiological pH values it is therefore present almost entirely as the univalent hypophosphite ion.
(15) When Escherichia coli cells were grown in media containing either phosphite or hypophosphite as the sole source of phosphorus, the responded to this situation primarily in the same way as phosphate-limited cultures: The activity of alkaline phosphatase increased drastically, which under natural conditions would enable the cells to compensate for the shortage of phosphate.
(16) We report here that the inactivation of both the free and acetylated forms of the lyase is subject to a primary kinetic isotope effect using [2H2]hypophosphite.
(17) capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from Cape Canerval soil samples.
(18) This enzyme is inhibited by low concentrations of potassium cyanide, copper sulphate and hypophosphite.
(19) Formaldehyde resistance can be drastically lowered down to 4 mM by blocking the formate dehydrogenase by means of hypophosphite.
(20) From a single 31P NMR spectrum it was possible to determine the relative amounts of hypophosphite in the intra- and extracellular compartments and thereby estimate the corresponding concentrations.
Phosphite
Definition:
(n.) A salt of phosphorous acid.
Example Sentences:
(1) The incorporation of phosphine and phosphite ligands is described.
(2) In connection with this study, deoxyribonucleoside-3' dimethyl phosphites were synthesized and detailed properties of them are also described.
(3) The passive net transport of Li+ and Na+ across the human red cell membrane was accelerated by the divalent anions carbonate, sulphite, oxalate, phosphite and malonate.
(4) A period of adaptation was required prior to growth on phosphite when phosphate-grown cells were transferred to a medium containing a limiting amount of phosphate and excess phosphite.
(5) No phosphite-oxidizing activity could be detected in whole cells or cell-free extracts of phosphate-grown cells.
(6) The pH maxima observed for the phosphate analogs indicate a pK for this site of 5.5 at 37 degrees C. Intracellular pH changes associated with influx indicated that transport of the "fast" anion phosphite is largely in monoionized form.
(7) Adaptation to hypophosphite, however, led simultaneously to phosphite adaptation, so that these cells can utilize both P-compounds as a substitute for phosphate.
(8) The data show that detritylation and oxidation are side reactions which occur during the synthesis of monomeric units used in the construction of oligodeoxyribonucleotides by the phosphite triester method.
(9) It was shown that all 5'-phosphites effectively inhibit the production of viral antigens and protect cells from the cytotoxic effect of HIV infection.
(10) 2-(2-Pyridyl)ethyl group is a new type P-O protecting group for the synthesis of oligodeoxyribonucleotides by the phosphite triester method.
(11) Trimethyl and triethyl phosphites have general toxic effects.
(12) The naturally occurring DNA-nucleopeptide H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH was prepared via a solid-phase phosphite triester approach using N-2-(tert-butyldiphenylsilyloxymethyl)benzoyl protected nucleosides.
(13) A gene coding for human stefin B was synthesized by the solid-phase phosphite method and cloned in the pUC8 cloning vector.
(14) Assays for phosphite removal by dilution or dialysis do not reverse the inhibition.
(15) The observed adaptation pattern, reflected by the alterations of phosphatase activity, was qualitatively equal with PO3-3 and PO3-2, but quantitatively different, because the response to hypophosphite gave much higher values than the increase obtained with phosphite.
(16) Data on the decreased toxicity of the phosphite mixture are explained from the viewpoint of a decreased pool disbalance of natural 2'-deoxynucleoside 5'-triphosphates in cells; a significant pool disbalance is developed in the case of 3'-azido-2',3'-dideoxythymidine action.
(17) Phosphonoacetaldehyde hydrolase inactivation by phosphite ion appears to be inconsistent with the concept of a Schiff base intermediate as proposed for Bacillus cereus enzyme.
(18) Reaction of 18 or 19 with triethyl phosphite gave, after deprotection, 6-(2,4-dichlorophenyl)-D-erythro-2,4-dihydroxyhexyl-phosphonic acid (5), and reaction of 19 with potassium cyanide gave, after subsequent hydrolysis and deprotection, 7-(2,4-dichlorophenyl)-D-erythro-3-hydroxy-5-heptanolide (3).
(19) Triphenyl phosphite (TPP)-induced delayed neurotoxicity, which is thought to resemble but somewhat differ from classical organophosphate-induced delayed neurotoxicity (OPIDN), is known to be age-dependent.
(20) Previous studies in mammals have found that exposure to triphenyl phosphite results in cellular and axonal degeneration in the spinal cord and medulla.