(n.) A glucoside obtained from woad (indigo plant) and other plants, as a yellow or light brown sirup. It has a nauseous bitter taste, a decomposes or drying. By the action of acids, ferments, etc., it breaks down into sugar and indigo. It is the source of natural indigo.
(n.) An indigo-forming substance, found in urine, and other animal fluids, and convertible into red and blue indigo (urrhodin and uroglaucin). Chemically, it is indoxyl sulphate of potash, C8H6NSO4K, and is derived from the indol formed in the alimentary canal. Called also uroxanthin.
Example Sentences:
(1) When applied as diagnostic tests for the stagnant loop syndrome, the phenol excretion showed 2 false negative results, the p-cresol excretion 3 false negative and 2 false positive results, and the indican excretion 6 false positive results.
(2) p-Cresol may therefore prove more sensitive than indican as an indicator of altered microbial metabolism due to saccharin.
(3) Intestinal protein metabolism and bile acid deconjugation (measured by urinary indican excretion and 14C-glycocholic acid breath test) was significantly enhanced in bypass patients.
(4) This diagnostic procedure was applied to the identification of patients with steatorrhoea due to the stagnant loop syndrome, and was compared with other tests for this condition, viz, study of the small intestinal bacterial flora, urinary indican excretion, and the Schilling test with added intrinsic factor.
(5) When Id and NaS were ingested together, the bladder mass increase was additive, but the epithelial hyperplasia was not exacerbated over that observed with each alone, and the urinary indican was equivalent to that produced by Id alone.
(6) Most indican excreted in the urine comes from the degradation of tryptophan through the action of microorganisms dwelling within the intestinal lumen.
(7) The colors are formed from the substrate indoxyl sulfate (indican) and all 7 patients had bacteria in the urine that would produce blue colonies on agar enriched with the urine (filter sterilized) of the patients involved.
(8) Determination of phenol and indican in a 24-hour urine sample is likely to provide a simple method for selecting patients with signs of abnormal bacterial colonization in the small intestine for more detailed investigations.
(9) A simple analytical procedure has been developed for the determination of indican and tryptophan in biological fluids by reversed-phase liquid chromatography using a new electrochemical detector consisting of a tubular anode obtained by moulding graphitized carbon black and polyethylene.
(10) The microbial catabolism of tryptophan to indole has been studied in 15 human subjects by analysis of the daily urinary excretion of indican (potassium indoxylsulphate) before, during and after chronic saccharin ingestion.
(11) Indican excretion was higher in patients with purple urinary catheter bags than in controls.
(12) By shortening the reaction time with the 2,4-DCPD reagent to 1.7 min, we find that the indican interference can be eliminated, without affecting quantification of total bilirubin in either normal or uremic sera.
(13) The excretion of indican (formed from indole, a microbial metabolite of tryptophan) was increased by saccharin in a dose-related fashion at all time points, but showed only a 3-fold increase at 7.5% compared with the 0% group.
(14) Increased excretion of indican has been noted, suggesting deficient intestinal absorption of L-tryptophan.
(15) The Jendrassik Bilirubin Reagent System (American Monitor) and a modified Jendrassik-Grof procedure (Hoffmann-LaRoche) adapted to the Cobas Bio analyzer were unaffected by the presence of indican.
(16) Treatment with pancreatic extract resulted in an immediate increase in indican excretion to above the normal range in patients with steatorrhoea due to pancreatic insufficiency.
(17) Saccharin-treated animals in both generations showed increased urinary excretion of indican.
(18) The urinary excretion of indican was increased in the fistula-operated rats, but further studies are needed to establish the significance of this observation.
(19) Indican also interfered with the Micro Bilirubin Reagent Set (Harleco) Malloy-Evelyn procedure, but to a much lesser extent.
(20) Concentrations of indican were about 1.5 those of tryptophan and considerably greater than those of indole-3-acetic acid in hemodialysate samples from 12 renal patients.
Sulphate
Definition:
(n.) A salt of sulphuric acid.
Example Sentences:
(1) Sixteen smooth Brucella strains were lysed and digested by proteinase K, and the LPS fractions analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
(2) The decomposition of nafcillin and penicillin G solutions was hastened significantly by magnesium sulphate due to effect on the pH values of the solutions.
(3) The K5 polysaccharide was N-deacetylated (by hydrazinolysis) and N-sulphated, and was then incubated with detergent-solubilized enzymes from a heparin-producing mouse mastocytoma, in the presence of adenosine 3'-phosphate 5'-phospho[35S] sulphate ([35S]PAPS).
(4) Plasma noradrenaline levels were depressed by 50% when demedullated fetuses were also subject to peripheral sympathectomy by guanethidine sulphate treatment.
(5) Chemically isolated separate preparations of the non-aggregating protein-chondroitin-keratin sulphate (PCKS) fraction from the hyaline cartilage and hyaluronic acid (HUA) of the vitreous body and of the umbilicus were investigated by electron microscopy.
(6) Granules in a few cells also contained sulphated mucin.
(7) Deficiency of glucosamine-6-sulphatase activity leads to the lysosomal storage of the glycosaminoglycan, heparan sulphate and the monosaccharide sulphate N-acetylglucosamine 6-sulphate and the autosomal recessive genetic disorder mucopolysaccharidosis type IIID.
(8) PT painting resulted in rather higher sensitivity with Triton X-100 than with sodium lauryl sulphate.
(9) For binding measurements self-diffusion equilibrium dialysis with dodecyl [35S] sulphate was used.
(10) We tested for the distribution of basement membrane (BM) components collagen IV, laminin, heparan sulphate proteoglycan, fibronectin, for S100 protein and for the presence of interstitial collagens III and V. Laminin was generally noted in association with Schwann cells, but collagen IV occurred with perineural cells.
(11) Ammonium sulphate fractionation followed by immunoaffinity chromatography on a three column system using Protein A-Sepharose coupled D5, produced purified p29.
(12) An enzyme (EC 2.8.2.1) that catalyses the transfer of sulphate from adenosine 3'-phosphate 5'-sulphatophosphate to phenols was purified approx.
(13) Platelet factor 4 was compared with protamine sulphate, which has similar biological properties, by electrophoresis at pH 2.2, in which both migrated as single bands but with differing mobility, and by amino acid analysis which showed a more normal distribution of residues than occurred in protamine sulphate.
(14) (a) Ammonium sulphate precipitation and gel filtration on Sepharose 4B.
(15) This inhibition was partially reversed on addition of the translocated substrates sulphate or selenate to the external medium: selenite which is not translocated does not protect against DIDS inhibition.
(16) Protamine sulphate in vitro antagonized anticoagulant properties but did not protect mice from toxic envenomation; because venom was also neurotoxic and showed a curare like effect at the neuromuscular junction.
(17) Degradation products of dermatan sulphate were not detected by either gel filtration or affinity chromatography on Polybrene-Sepharose at any time in either plasma or urine, indicating that administered dermatan sulphate is not catabolised by man.
(18) Internal alkalinization could also be induced by oleate upon the addition of potassium sulphate.
(19) Addition of rising concentrations of zinc sulphate to rat PRP produced inhibition of ADP-induced platelet aggregation.
(20) Both surfactants were extensively degraded in vivo to yield a common metabolite, butyric acid 4-[35S]sulphate, the major urinary radioactive component.