(1) The feasibility of using fluorescent ISH for sexing biopsied embryos in couples at risk of X-linked disease and for the preimplantation diagnosis of chromosome abnormalities is discussed.
(2) The two patients without ISH evidence of EBV infection showed no distinctive clinical, morphologic, or serologic features.
(3) 5-bromo-2'-deoxyuridine (BrdU) to oligonucleotide probes guaranteed the production of labelled probes of 100% specific activity that gave consistent, reproducible results, within hours, in a rapid in situ hybridization (ISH) technique.
(4) The cases found positive by IHC showed brownish nuclei of the epithelium and those positive in ISH showed purple to purplish-black nuclei.
(5) Specimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ISH) with biotinylated DNA probes for human papillomavirus (HPV) types 6, 11, 16 and 18.
(6) Quantification of the fluorescence ISH signals was performed using an epi-fluorescence microscope with a multi-wavelength illuminator, equipped with a cooled charge couple device (CCD) camera.
(7) By radioactive in situ hybridization (ISH) using a fragment from the murine Pax-1 paired box that is almost identical to the respective sequences from the cognate human gene HuP48 and fluorescence in situ hybridization (FISH) using a complete mouse Pax-1 cDNA, we have assigned the human homologue of murine Pax-1, the PAX1 locus, to chromosome 20p.
(8) These results suggest that the BP of ISH patients is susceptible to fluctuations in autonomic nerve activity.
(9) Plasma immunoreactive atrial natriuretic factor (ANF) and urinary sodium excretion were measured in elderly patients with isolated systolic hypertension (ISH) (n = 11), age-matched essential hypertensive patients (EHT; n = 16) and normotensive subjects (NT; n = 9) before and during a 60 min infusion of hypertonic saline (120 mEq of Na+).
(10) Among pro-independence people there are widespread concerns that if the SNP moves too quickly on a referendum it will cast the choice in the often bland New Labour-ish terms it uses for everyday politics – and thus deprive Scotland of a crucial opportunity to discuss its future, as well as threatening their chances of winning.
(11) The Gogglebox people are all nice(ish) and funny(ish), qualities vital to keep at bay total self-loathing that we are gathered as a family, watching on telly other people watching telly.
(12) Also, all other tumours investigated, including thymomas, thymic carcinomas, tonsillar carcinomas and medullary breast carcinomas were negative upon ISH to EBV-specific probes.
(13) Work on The Maze Runner came about, he says, because his director watched Son of Rambow “and knew I had some bully-ish qualities in my acting locker”.
(14) If the WHO-ISH criteria are accepted as the standard for deciding to treat patients with mild hypertension the predictive value of one ambulatory blood pressure recording over 12 hours is too low to detect with confidence those patients who need treatment when managed according to these criteria.
(15) Indeed, such parochialism would be downright frowned upon by today's World Cup mentality, considering that both the official anthem and slogan this time round is the typically Fifa-ishly nonsensical, and distinctly Benetton-esque, "We Are One".
(16) To take a Marx-ish view of all this, its roots are clearly economic: the demise of the large-scale industrial economy that reached its peak just after the second world war, and the consequent weakening of the idea of politics as a battle between two huge ideological blocs.
(17) There were significant associations between the detection of rhinoviruses by ISH and virus isolation on the third day (P less than 0.025) after virus challenge in the group as a whole and in the symptomatic group.
(18) Jan Jan is actually not a bad tune, with distinctive Anastacia-ish vocals being the highlight (alongside a fat bloke formation dancing in the video).
(19) Sorrell warns ad industry against 'Don Draper-ish' optimism as Brexit vote looms Read more The company confirmed this week it would announce that from 2011 to 2015 WPP had outperformed its peers and the FTSE 100.
(20) ISH technique has been applied and is one of the most powerful strategies in the biological research field of psychiatry.
(adv.) In a rough manner; unevenly; harshly; rudely; severely; austerely.
(1) By 24 hr, rough endoplasmic reticulum in thecal cells increased from 4.2 to 7% of cell volume, while the amount in granulosa cells increased from less than 3.5% to more than 10%; the quantity remained relatively constant in the theca but declined to prestimulation values in the granulosa layer.
(2) Thus, it appears that neuronal loss may account for up to roughly half of the striatal D2 receptor loss during aging.
(3) The cis isomer was retained longer in liver, particularly in mitochondria, but had low retention in that portion of the endoplasmic reticulum isolated as the rough membrane fraction.
(4) The results indicated that roughly 25% of patients treated in this way will become hypothyroid after 5 years and that 85% are cured (need no further therapy during the follow-up period) using a single dose of iodine-131.
(5) This heretogeneity occurred mainly as a progressive, decreasing gradient in the first half of this pathway, between the rough endoplasmic reticulum and the mi-cisternae of the Golgi apparatus.
(6) Electron microscopy revealed a well-developed rough endoplasmic reticulum, an enlarged Golgi apparatus and many highly electron-dense secretory granules resembling those of Clara cells.
(7) Four fractions enriched, respectively, in plasma membrane (PM), smooth endoplasmic reticulum (SER), rough endoplasmic reticulum (RER), and mitochondria were isolated from estrogen-dominated rat myometrium.
(8) For trials in which the target was present in the array, RT functions were roughly symmetric, the shortest RTs being for extreme distractor ratios, and the longest RTs being for arrays in which there were an equal number of each distractor type.
(9) Classic technics of digital image analysis and new algorithms were used to improve the contrast on the full image or a portion of it, contrast a skin lesion with statistical information deduced from another lesion, evaluate the shape of the lesion, the roughness of the surface, and the transition region from the lesion to the normal skin, and analyze a lesion from the chromatic point of view.
(10) Electron microscopic evaluation of microsomal fractions showed elements of the plasma membrane, including cilia and microvilli, as well as rough and smooth endoplasmic reticulum.
(11) The local guide led us down a rough, uneven pathway, talking as he went.
(12) It's the roughly $2bn in revenue grossed by his blockbuster movies, some of which he had to be talked into making.
(13) The interaction between PE and E-IgG involved the extension of micropseudopods toward adherent E-IgG, the formation of a linear uniform cap of roughly 200 A between opposing cell membranes, the ingestion of E-IgG by PE into a membrane-lined compartment, and the disintegration of the ingested ligand into membranous debris.
(14) Ultracentrifugally separated HDL2 and HDL3 roughly corresponded to HDL2e and HDL3e, respectively.
(15) The locations of these 15 insertion sites correlate well with the roughly estimated locations of five of the DNase I-hypersensitive subregions.
(16) The Lords will vote on three key amendments: • To exclude child benefit from the cap calculation (this would roughly halve the number of households affected).
(17) The unique structure we describe is a cytoplasmic organelle which, like annulate lamellae, is closely associated with the endoplasmic reticulum and is presumed to be related to the genesis of rough-surfaced endoplasmic reticulum in tumor cells.
(18) Besides the rough, wrinkled, and brown or black surface of the fingertips, microwrinkles of the epidermis occur on the skin ridges, which have so far not been described.
(19) Ultrastructural examination of noncartilaginous regions of the tumor demonstrated mesenchymal cells with features suggestive of cartilaginous differentiation, viz, scalloped cell membranes, sac-like distension of abundant rough endoplasmic reticulum, and a matrix containing fibrillary and finely granular material.
(20) That, roughly, was the theme of the Wednesday Play, Cathy Come Home, (BBC1) directed by Kenneth Loach, produced by Tony Garnett.