(1) This enhancer sequence is not strictly cell-type specific since it functions in cell lines of both placental (JEG-3) and pituitary (18-54,SF) origin.
(2) VIP maximally increased the transient expression of CRE alpha CAT and the expression of endogenous hCG-alpha mRNA in JEG-3 cells by 4- and 9-fold, respectively.
(3) This result may be explained by the observation that the JAr cells lacked any detectable glucocorticoid receptor and the receptor levels in cytosol prepared from JEG and BeWo cells were 12% and 2%, respectively, of those measured in HeLa cytosol.
(4) The HLA-6.0 or HLA-E genes may encode the lower molecular mass component on Jeg-3 cells, because these genes express molecules of about 38 kDa and 41 kDa, respectively, when transfected to appropriate host cells.
(5) In order to determine whether RA stimulates the hCG secretion by other trophoblastic cell lines, we evaluated the effect of RA on hCG, hCG-alpha subunit (hCG-alpha), and progesterone secretion in three choriocarcinoma cell lines: JEG-3, JAR, and BeWo.
(6) Reporter genes containing either the intact 1.5 kilobase of 5'-flanking sequence from the HCG alpha gene, or just the 36-bp tandem repeat cAMP response element, when coupled to an enhancerless promoter, both exhibit a vigorous cAMP stimulation of expression but no response to phorbol ester in JEG-3 cells.
(7) We demonstrate the molecular peculiarities of these modified smooth muscle cells using monoclonal antibodies produced against a benign human JEG-cell tumour.
(8) The transcriptional activities of P1 + P2 in their natural tandem orientation were additive in rat keratinocytes and human JEG choriocarcinoma cells.
(9) Using DNase I protection analyses and gel-mobility shift assays, we examined in vitro the domains in the human alpha-gene 5'-flanking sequence that bind nuclear proteins extracted from JEG-3 choriocarcinoma cells.
(10) The effect of JEG-3 choriocarcinoma supernatant on human natural killer cell and lymphokine-activated killer cell activity was investigated.
(11) Similar observations have been made in JeG-3 cells and Chinese hamster ovary cells stably transfected with human apoE cDNA constructs.
(12) In human choriocarcinoma JEG-3 and monkey COS-1 cells, the cytosolic thyroid hormone binding protein is a monomer of the tetrameric pyruvate kinase, subtype M2, which does not bind T3.
(13) In this study the 5'-flanking sequence of the CG beta gene was fused to a sensitive luciferase (LUC) reporter gene, allowing delineation of a CG beta CRE in transient expression assays performed in JEG-3 choriocarcinoma cells.
(14) JEG-3 choriocarcinoma cells demonstrate c-myc transcript levels that are greater than those of nonmalignant trophoblastic tissue at any stage of gestation.
(15) No significant expression of any of these ANG-GH fusion genes over the promoterless pOGH was observed in JEG-3 cells.
(16) Results of these studies suggest that there is some degree of tissue specificity of FN gene expression and reveal that cAMP induction is mediated, in part, by the same element (CRE) in both HT-1080 and JEG-3 cells.
(17) Epidermal growth factor (EGF) binds to JEG-3 cells, a tissue culture line of human choriocarcinoma.
(18) In JEG-3 choriocarcinoma cells the MMTV promoter is transcribed inefficiently, even after transfection of the receptor cDNA and treatment with glucocorticoids or progestins.
(19) A similar increase in Class I antigens is also found in JEG-3 choriocarcinoma cells after treatment with IFN-gamma.
(20) Deletion of the FN promoter from positions -510 to -56 resulted in an ~30-fold decrease in promoter activity when this construction was transfected into HT-1080 cells, and similar results were observed in NIH 3T3 cells; however, less than a 2-fold effect was observed in JEG-3 cells.
Jig
Definition:
(n.) A light, brisk musical movement.
(n.) A light, humorous piece of writing, esp. in rhyme; a farce in verse; a ballad.
(n.) A piece of sport; a trick; a prank.
(n.) A trolling bait, consisting of a bright spoon and a hook attached.
(n.) A small machine or handy tool
(n.) A contrivance fastened to or inclosing a piece of work, and having hard steel surfaces to guide a tool, as a drill, or to form a shield or templet to work to, as in filing.
(n.) An apparatus or a machine for jigging ore.
(v. t.) To sing to the tune of a jig.
(v. t.) To trick or cheat; to cajole; to delude.
(v. t.) To sort or separate, as ore in a jigger or sieve. See Jigging, n.
(n.) To cut or form, as a piece of metal, in a jigging machine.
(v. i.) To dance a jig; to skip about.
Example Sentences:
(1) If Del Bosque really want to win this World Cup thingymebob, then he has got to tell Iker Casillas that the jig is up, correct?
(2) Between members of different teams however, only the finger breadth method attained reliabilities above .7, and the plexiglass jig, in particular, showed very low reliability.
(3) A new jig simulating the abdominal cavity and wall is described.
(4) The stain pattern on the stem was analysed in a 3-point-bending jig and also after cementing it into cadaver femurs.
(5) Dipnets and jigs inflicted minimal trauma and were preferred for squid capture.
(6) Once the jig is in position, the patient is asked to produce left and right lateral movements until muscular relaxation is obtained.
(7) We developed some instruments to resolve these problems; i.e., scopes with a large diameter for high resolution, a triangulation instrument for multiple cannulations, a needle set-up jig for disk traction suture, a step cannulation system and a two-channel cannula for operating in the narrow lower joint space and a fixing jig for cannulas in the upper and lower joint space to observe the same portion of the discal tissue from both joint space during disk suturing.
(8) When, in 1996, the Globe theatre in London first experimented with doing it Shakespeare’s way, the scholars and theatre folk encountered the jig problem.
(9) This study used a special jig system, photography, and a sonic digitizer to evaluate the change in canal size and location after retreatment in 20 teeth with small or large curved canals (greater than 23 degrees).
(10) The kinetics of neural uptake and efflux of lidocaine hydrochloride were studies by means of a standardized technique for blocking the intraorbital nerve of the rat, using a palatal jig.
(11) Thermostatic regulation of tissue temperature is provided by on-off control of the average power supplied independently to each heating jig.
(12) It is recommended that the clearance of the hole in the support jig is at least 0.7 mm and that push-out results are only compared with each other when materials with similar Young's modulus are concerned.
(13) The ads have featured a miniature Miliband in Salmond’s jacket pocket and, in one animated film, the Labour leader dancing to a Scottish jig played by Salmond on a recorder.
(14) When a mechanical checkout jig was set up at the same point, a discrepancy of 4 mm resulted when the gantry was moved from 0 degrees to 180 degrees.
(15) The Herbert screw is useful in treating displaced capitellar fractures since the jig maintains the reduction and the screw, compressing the fracture site, is buried beneath the articular cartilage and does not have to be removed.
(16) If you want sustainable supply chains, you have to re-jig how you think.
(17) A two-part German-South African co-production based on the bestselling Kate Mosse novel, it's a window-rattling potboiler bubbling with ancient religious conspiracies, comely medieval wenches, comely 21st-century academics, fogbanks of swirly past-times skulduggery, evil pharmaceutical CEOs in 10 denier tights, priapic chevaliers and, verily, a script that does dance a merry jig upon the very phizog of credibility.
(18) A central distractor, attached to the jig, positions and aligns the knee at 0 degrees or 90 degrees.
(19) A pilot drill with a built-in stop to prevent overpenetration is used first and then the screw can be inserted with jig, or it may be inserted manually if the osteotomy is stablized temporarily with a Kirschner wire.
(20) Single-limb-stance loads and combined axial and torsional loads were applied to the implanted femoral prostheses with the use of a jig that simulated acetabular and trochanteric loading.