(1) This enhancer sequence is not strictly cell-type specific since it functions in cell lines of both placental (JEG-3) and pituitary (18-54,SF) origin.
(2) VIP maximally increased the transient expression of CRE alpha CAT and the expression of endogenous hCG-alpha mRNA in JEG-3 cells by 4- and 9-fold, respectively.
(3) This result may be explained by the observation that the JAr cells lacked any detectable glucocorticoid receptor and the receptor levels in cytosol prepared from JEG and BeWo cells were 12% and 2%, respectively, of those measured in HeLa cytosol.
(4) The HLA-6.0 or HLA-E genes may encode the lower molecular mass component on Jeg-3 cells, because these genes express molecules of about 38 kDa and 41 kDa, respectively, when transfected to appropriate host cells.
(5) In order to determine whether RA stimulates the hCG secretion by other trophoblastic cell lines, we evaluated the effect of RA on hCG, hCG-alpha subunit (hCG-alpha), and progesterone secretion in three choriocarcinoma cell lines: JEG-3, JAR, and BeWo.
(6) Reporter genes containing either the intact 1.5 kilobase of 5'-flanking sequence from the HCG alpha gene, or just the 36-bp tandem repeat cAMP response element, when coupled to an enhancerless promoter, both exhibit a vigorous cAMP stimulation of expression but no response to phorbol ester in JEG-3 cells.
(7) We demonstrate the molecular peculiarities of these modified smooth muscle cells using monoclonal antibodies produced against a benign human JEG-cell tumour.
(8) The transcriptional activities of P1 + P2 in their natural tandem orientation were additive in rat keratinocytes and human JEG choriocarcinoma cells.
(9) Using DNase I protection analyses and gel-mobility shift assays, we examined in vitro the domains in the human alpha-gene 5'-flanking sequence that bind nuclear proteins extracted from JEG-3 choriocarcinoma cells.
(10) The effect of JEG-3 choriocarcinoma supernatant on human natural killer cell and lymphokine-activated killer cell activity was investigated.
(11) Similar observations have been made in JeG-3 cells and Chinese hamster ovary cells stably transfected with human apoE cDNA constructs.
(12) In human choriocarcinoma JEG-3 and monkey COS-1 cells, the cytosolic thyroid hormone binding protein is a monomer of the tetrameric pyruvate kinase, subtype M2, which does not bind T3.
(13) In this study the 5'-flanking sequence of the CG beta gene was fused to a sensitive luciferase (LUC) reporter gene, allowing delineation of a CG beta CRE in transient expression assays performed in JEG-3 choriocarcinoma cells.
(14) JEG-3 choriocarcinoma cells demonstrate c-myc transcript levels that are greater than those of nonmalignant trophoblastic tissue at any stage of gestation.
(15) No significant expression of any of these ANG-GH fusion genes over the promoterless pOGH was observed in JEG-3 cells.
(16) Results of these studies suggest that there is some degree of tissue specificity of FN gene expression and reveal that cAMP induction is mediated, in part, by the same element (CRE) in both HT-1080 and JEG-3 cells.
(17) Epidermal growth factor (EGF) binds to JEG-3 cells, a tissue culture line of human choriocarcinoma.
(18) In JEG-3 choriocarcinoma cells the MMTV promoter is transcribed inefficiently, even after transfection of the receptor cDNA and treatment with glucocorticoids or progestins.
(19) A similar increase in Class I antigens is also found in JEG-3 choriocarcinoma cells after treatment with IFN-gamma.
(20) Deletion of the FN promoter from positions -510 to -56 resulted in an ~30-fold decrease in promoter activity when this construction was transfected into HT-1080 cells, and similar results were observed in NIH 3T3 cells; however, less than a 2-fold effect was observed in JEG-3 cells.
Jug
Definition:
(n.) A vessel, usually of coarse earthenware, with a swelling belly and narrow mouth, and having a handle on one side.
(n.) A pitcher; a ewer.
(n.) A prison; a jail; a lockup.
(v. t.) To seethe or stew, as in a jug or jar placed in boiling water; as, to jug a hare.
(v. t.) To commit to jail; to imprison.
(v. i.) To utter a sound resembling this word, as certain birds do, especially the nightingale.
(v. i.) To nestle or collect together in a covey; -- said of quails and partridges.
Example Sentences:
(1) The tinsel coiled around a jug of squash and bauble in the strip lighting made a golf-ball size knot of guilt burn in my throat.
(2) Allow to cool slightly for a few minutes before serving, with a jug of chilled cream alongside.
(3) Priapic gadabouts in peephole codpieces hey-nonny-no-ing past plates of glazed pig as smouldering flibbertigibbets pout and motion to their jugs.
(4) Our kind waiter, Paul, delighted our tot with her own special jug and cup, and steaming bowlfuls of spätzle pasta.
(5) You will never see cream in my house that is not in a jug, nor salt that is not in a cellar.
(6) I requested a jug from the nurse but she said the jug was broken and they had no others available.
(7) They took the term skiffle from a favourite record, Home Town Skiffle, a compilation of American jug band styles and western swing.
(8) I'm not too well up on the Middle Eastern judicial system, but couldn't he get slung in the jug for a very long time for that?
(9) "Look – Putin didn't find down there jugs that had lain there for many thousands of years.
(10) If anything, his brother David looks more like Wallace because he really does have Wallace-style jug ears.
(11) When a glass+jug (900 ml) was visible the alcoholics drank significantly more than the non-alcoholics.
(12) The product was jugged to be galactonic acid, based on the behavior of the acetylmethyl ester derivative of the product and the pentaacetyl derivative of the galactonic methyl ester during gas chromatography.
(13) During one technical challenge, I saw one baker use, at the very least, six glass bowls, a saucepan, a sieve, a spatula, a silicon sheet, spoons, a pastry brush, a skewer, a cake tin, palette knives, piping bags, a measuring jug, scissors, a rolling pin, spoons and a cooling rack.
(14) Earlier this year, Waitrose reported that sales of 1 litre mixing bowls had more than doubled, measuring jug sales had quadrupled and rolling pins were up 40% .
(15) A row of Toby jugs grinned and grimaced from an ornament rail in the hall.
(16) Still employed in the early 1990s, the classic label sported a blue-and-white striped milk jug beside two cherry-red mugs, resting on sheaves of wheat, against an luminous yellow arc of - well, obviously, an incandescent light bulb.
(17) I was disciplined for not changing the water often enough for a woman I was caring for despite the jug never being less than half full.
(18) Thymol mouthwash which had been made up and distributed in communal jugs was found to be contaminated with the epidemic strain and was the likely source for this outbreak.
(19) The woman declined an offer to post the jugs back to her afterwards, and the constable now has one "at home as a little keepsake because I thought it was such a nice gesture".
(20) He is, for instance, technically taller than Martin Freeman but not by much more than a jug of Bree's finest hobbit ale.