(1) This enhancer sequence is not strictly cell-type specific since it functions in cell lines of both placental (JEG-3) and pituitary (18-54,SF) origin.
(2) VIP maximally increased the transient expression of CRE alpha CAT and the expression of endogenous hCG-alpha mRNA in JEG-3 cells by 4- and 9-fold, respectively.
(3) This result may be explained by the observation that the JAr cells lacked any detectable glucocorticoid receptor and the receptor levels in cytosol prepared from JEG and BeWo cells were 12% and 2%, respectively, of those measured in HeLa cytosol.
(4) The HLA-6.0 or HLA-E genes may encode the lower molecular mass component on Jeg-3 cells, because these genes express molecules of about 38 kDa and 41 kDa, respectively, when transfected to appropriate host cells.
(5) In order to determine whether RA stimulates the hCG secretion by other trophoblastic cell lines, we evaluated the effect of RA on hCG, hCG-alpha subunit (hCG-alpha), and progesterone secretion in three choriocarcinoma cell lines: JEG-3, JAR, and BeWo.
(6) Reporter genes containing either the intact 1.5 kilobase of 5'-flanking sequence from the HCG alpha gene, or just the 36-bp tandem repeat cAMP response element, when coupled to an enhancerless promoter, both exhibit a vigorous cAMP stimulation of expression but no response to phorbol ester in JEG-3 cells.
(7) We demonstrate the molecular peculiarities of these modified smooth muscle cells using monoclonal antibodies produced against a benign human JEG-cell tumour.
(8) The transcriptional activities of P1 + P2 in their natural tandem orientation were additive in rat keratinocytes and human JEG choriocarcinoma cells.
(9) Using DNase I protection analyses and gel-mobility shift assays, we examined in vitro the domains in the human alpha-gene 5'-flanking sequence that bind nuclear proteins extracted from JEG-3 choriocarcinoma cells.
(10) The effect of JEG-3 choriocarcinoma supernatant on human natural killer cell and lymphokine-activated killer cell activity was investigated.
(11) Similar observations have been made in JeG-3 cells and Chinese hamster ovary cells stably transfected with human apoE cDNA constructs.
(12) In human choriocarcinoma JEG-3 and monkey COS-1 cells, the cytosolic thyroid hormone binding protein is a monomer of the tetrameric pyruvate kinase, subtype M2, which does not bind T3.
(13) In this study the 5'-flanking sequence of the CG beta gene was fused to a sensitive luciferase (LUC) reporter gene, allowing delineation of a CG beta CRE in transient expression assays performed in JEG-3 choriocarcinoma cells.
(14) JEG-3 choriocarcinoma cells demonstrate c-myc transcript levels that are greater than those of nonmalignant trophoblastic tissue at any stage of gestation.
(15) No significant expression of any of these ANG-GH fusion genes over the promoterless pOGH was observed in JEG-3 cells.
(16) Results of these studies suggest that there is some degree of tissue specificity of FN gene expression and reveal that cAMP induction is mediated, in part, by the same element (CRE) in both HT-1080 and JEG-3 cells.
(17) Epidermal growth factor (EGF) binds to JEG-3 cells, a tissue culture line of human choriocarcinoma.
(18) In JEG-3 choriocarcinoma cells the MMTV promoter is transcribed inefficiently, even after transfection of the receptor cDNA and treatment with glucocorticoids or progestins.
(19) A similar increase in Class I antigens is also found in JEG-3 choriocarcinoma cells after treatment with IFN-gamma.
(20) Deletion of the FN promoter from positions -510 to -56 resulted in an ~30-fold decrease in promoter activity when this construction was transfected into HT-1080 cells, and similar results were observed in NIH 3T3 cells; however, less than a 2-fold effect was observed in JEG-3 cells.
Keg
Definition:
(n.) A small cask or barrel.
Example Sentences:
(1) Photograph: supplied Nauru: a powder keg waiting to ignite All the signs suggest a moment of crisis is approaching on Nauru .
(2) But more rounded beer fans will find plenty to enjoy in its vast array of bottles (a bit bewildering, as there was no menu on a recent visit) and 13 keg lines.
(3) On the front line in Brazil’s war on Zika: ‘I felt I was in a horror movie’ Read more In global health security terms, this is a potential powder keg.
(4) Eight cask pumps showcase Leeds beers (try the pale or the dark mild, Midnight Bell) and four guests, with keg lines, such as a craft lager from York micro Hop Studio, adding interest.
(5) Taiwan is another powder keg that could be ignited by widening US-China confrontation.
(6) Across eight cask pumps, seven keg lines and three hand-pulled ciders, the Rook runs the gamut from exotic European imports (Opat's self-explanatory orange and mandarin Czech pils) to beers from lesser-spotted UK micros, such as Grafters and Jurassic Brewhouse.
(7) The molecule resembles a keg 105 A along the 4-fold axis and 132 A in diameter at the widest point of the keg.
(8) The brain tumor models were produced in syngeneic Wister-King-Aptekman male rats with stereotaxic inoculation of ethylnitrosourea-induced glioma cells (KEG-1).
(9) Quality beers are now being produced in kegs, bottles and even cans, helping craft brewers offset the rapid decline of high street pubs.
(10) Many craft beers, including BrewDog’s, do not qualify as real ale under Camra’s strict criteria simply because, although some are served from casks, most come in kegs, bottles and cans, and with added CO2.
(11) Long before Brewdog opened, Port Street was already turning Mancunians on to explosively hoppy US imports, edgy craft keg beers and impoverishing those who couldn't resist that second bottle of Alesmith Speedway Stout (750ml, £18).
(12) Prices for the craft keg beers (from £4.60 a pint) can get silly, but it goes with the territory.
(13) This real ale redoubt for dissenting Village drinkers serves six cask ales (from local outfits such as Little Valley, Beartown, Dunham Massey, etc), two craft keg beers from Bury's Outstanding and a short, solid list of imported bottled beers, including Flying Dog's Raging Bitch and Brooklyn Black Chocolate Stout.
(14) Looking back, that was a powder keg waiting for a match.
(15) Many in Camra still believe that cask is good and keg is bad.
(16) Others include Blackshore Stout and a dry hopped keg lager.
(17) She felt the lack of political will to tackle public health issues was a powder keg waiting to explode in one way or the other,” said her brother Albert, a GP based in the UK.
(18) This article reviews the most recent literature and discusses the author's "powder keg and tinderbox" theory of idiopathic calcium stone disease.
(19) It’s that people will choose not to buy something that they don’t like or want.” Ryan claims to have been dreaming of slashing social programs since his days doing keg stands, and when he and other Republicans managed to push the similarly disastrous American Health Care Act through the House, they wheeled out cases of beer to celebrate.
(20) Feral Hop Hog IPA, Swan Valley, WA It was only three years ago that a Melbourne pub, the Great Northern Hotel, first secured a few kegs of beer from WA’s Feral Brewing.