(1) This enhancer sequence is not strictly cell-type specific since it functions in cell lines of both placental (JEG-3) and pituitary (18-54,SF) origin.
(2) VIP maximally increased the transient expression of CRE alpha CAT and the expression of endogenous hCG-alpha mRNA in JEG-3 cells by 4- and 9-fold, respectively.
(3) This result may be explained by the observation that the JAr cells lacked any detectable glucocorticoid receptor and the receptor levels in cytosol prepared from JEG and BeWo cells were 12% and 2%, respectively, of those measured in HeLa cytosol.
(4) The HLA-6.0 or HLA-E genes may encode the lower molecular mass component on Jeg-3 cells, because these genes express molecules of about 38 kDa and 41 kDa, respectively, when transfected to appropriate host cells.
(5) In order to determine whether RA stimulates the hCG secretion by other trophoblastic cell lines, we evaluated the effect of RA on hCG, hCG-alpha subunit (hCG-alpha), and progesterone secretion in three choriocarcinoma cell lines: JEG-3, JAR, and BeWo.
(6) Reporter genes containing either the intact 1.5 kilobase of 5'-flanking sequence from the HCG alpha gene, or just the 36-bp tandem repeat cAMP response element, when coupled to an enhancerless promoter, both exhibit a vigorous cAMP stimulation of expression but no response to phorbol ester in JEG-3 cells.
(7) We demonstrate the molecular peculiarities of these modified smooth muscle cells using monoclonal antibodies produced against a benign human JEG-cell tumour.
(8) The transcriptional activities of P1 + P2 in their natural tandem orientation were additive in rat keratinocytes and human JEG choriocarcinoma cells.
(9) Using DNase I protection analyses and gel-mobility shift assays, we examined in vitro the domains in the human alpha-gene 5'-flanking sequence that bind nuclear proteins extracted from JEG-3 choriocarcinoma cells.
(10) The effect of JEG-3 choriocarcinoma supernatant on human natural killer cell and lymphokine-activated killer cell activity was investigated.
(11) Similar observations have been made in JeG-3 cells and Chinese hamster ovary cells stably transfected with human apoE cDNA constructs.
(12) In human choriocarcinoma JEG-3 and monkey COS-1 cells, the cytosolic thyroid hormone binding protein is a monomer of the tetrameric pyruvate kinase, subtype M2, which does not bind T3.
(13) In this study the 5'-flanking sequence of the CG beta gene was fused to a sensitive luciferase (LUC) reporter gene, allowing delineation of a CG beta CRE in transient expression assays performed in JEG-3 choriocarcinoma cells.
(14) JEG-3 choriocarcinoma cells demonstrate c-myc transcript levels that are greater than those of nonmalignant trophoblastic tissue at any stage of gestation.
(15) No significant expression of any of these ANG-GH fusion genes over the promoterless pOGH was observed in JEG-3 cells.
(16) Results of these studies suggest that there is some degree of tissue specificity of FN gene expression and reveal that cAMP induction is mediated, in part, by the same element (CRE) in both HT-1080 and JEG-3 cells.
(17) Epidermal growth factor (EGF) binds to JEG-3 cells, a tissue culture line of human choriocarcinoma.
(18) In JEG-3 choriocarcinoma cells the MMTV promoter is transcribed inefficiently, even after transfection of the receptor cDNA and treatment with glucocorticoids or progestins.
(19) A similar increase in Class I antigens is also found in JEG-3 choriocarcinoma cells after treatment with IFN-gamma.
(20) Deletion of the FN promoter from positions -510 to -56 resulted in an ~30-fold decrease in promoter activity when this construction was transfected into HT-1080 cells, and similar results were observed in NIH 3T3 cells; however, less than a 2-fold effect was observed in JEG-3 cells.
Peg
Definition:
(n.) A small, pointed piece of wood, used in fastening boards together, in attaching the soles of boots or shoes, etc.; as, a shoe peg.
(n.) A wooden pin, or nail, on which to hang things, as coats, etc. Hence, colloquially and figuratively: A support; a reason; a pretext; as, a peg to hang a claim upon.
(n.) One of the pins of a musical instrument, on which the strings are strained.
(n.) One of the pins used for marking points on a cribbage board.
(n.) A step; a degree; esp. in the slang phrase "To take one down peg."
(v. t.) To put pegs into; to fasten the parts of with pegs; as, to peg shoes; to confine with pegs; to restrict or limit closely.
(v. t.) To score with a peg, as points in the game; as, she pegged twelwe points.
(v. i.) To work diligently, as one who pegs shoes; -- usually with on, at, or away; as, to peg away at a task.
Example Sentences:
(1) Plasma renin activities (PRA) and aldosterone concentrations increased in parallel over a wide range of plasma volume deficits produced in unanesthetized rats by extravascular administration of polyethylene glycol (PEG) solution.
(2) To determine whether long-term enteral feedings can improve nutritional status and lung function parameters in patients with cystic fibrosis (CF), 11 patients (8 female, 3 male, age 7 to 23 years) received a percutaneous endoscopic gastrostomy (PEG) since February 1988.
(3) Advisable in a first time for the feeding of patients with palliative treatment, we propose PEG for patients in position to have a long and difficult rehabilitation of swallowing.
(4) Metoprolol was introduced into the stomach with a homogenized meal containing a nonabsorbable marker, [14C]-PEG 4000, and another marker, PEG 4000, was perfused continuously into the duodenum just below the pylorus.
(5) Decreased consistency of the stools was seen after PEG in both groups (p < 0.001).
(6) Since PEG-1000 treatment of HPRT- Chinese hamster cells in the absence of human cells yielded no HPRT+ cells, it is concluded that the element responsible for the restoration of rodent HPRT was contributed by the human cells and not by the agent employed to promote fusion.
(7) The CD spectra of these aggregates showed psi-type anomalies and intensities 10-100 times greater than those obtained with the dispersed DNA solutions in the absence of PEG.
(8) We next tried to prepare virus-free PEG-PLP-Hb from HBV or HTLV-I positive blood.
(9) The yes camp should have made no bones about a call to the nation to shake things up, by bringing him down a peg or two.
(10) The fast process in the presence of PEG was identified as due to rapid interbilayer monomer diffusion between closely apposed vesicles, and, in the absence of PEG, as due to monomer diffusion through the aqueous phase.
(11) Since it was first described Percutaneous Endoscopic Gastrostomy (PEG) has rapidly become the preferred method for gastrostomy tube placement.
(12) Percutaneous endoscopic gastrostomy (PEG) has become a commonly performed procedure to provide nutritional support to chronically ill patients.
(13) CIC-PEG and TRAb levels were similar in newly diagnosed and relapsed patients, being higher than in controls (p less than 0.01) and in remission patients (p less than 0.01).
(14) At a second operation, 10 days later, these adhesions were graded and lysed, after which the animals received one of the following solutions intraperitoneally: 5 per cent PEG 4000 (n = 21), 25 per cent PEG 4000 (n = 23), 32 per cent dextran 70 (n = 22) or isotonic saline (n = 25), or were left as an untreated control group (n = 20).
(15) The main histological features of the tumour were enormous, but relatively regular, acanthosis of rete pegs revealing no similarity to the squamous-cell carcinoma, and an exclusively parakeratottic eleidine-containing central plug.
(16) One hundred thirty-six percutaneous endoscopic gastrostomies (PEGs) were placed in 126 patients with head and neck malignancies.
(17) Sera from patients (n = 24) with hemolytic transfusion reactions and no detectable antibody by routine technics were tested; two sera had specific antibodies by the PEG technic.
(18) The PEG derivatization of enzymes with this procedure is less inactivating than those previously reported.
(19) Both liposome-mediated delivery and PEG conjugation offer an additional benefit over native superoxide dismutase and catalase because they can increase cellular antioxidant activities in a manner that can provide protection from both intracellular and extracellular superoxide and hydrogen peroxide.
(20) Both from diagnostic and prognostic points of view, PEG is of less value is communicating hydrocephalus on account of the many false findings.