(1) This enhancer sequence is not strictly cell-type specific since it functions in cell lines of both placental (JEG-3) and pituitary (18-54,SF) origin.
(2) VIP maximally increased the transient expression of CRE alpha CAT and the expression of endogenous hCG-alpha mRNA in JEG-3 cells by 4- and 9-fold, respectively.
(3) This result may be explained by the observation that the JAr cells lacked any detectable glucocorticoid receptor and the receptor levels in cytosol prepared from JEG and BeWo cells were 12% and 2%, respectively, of those measured in HeLa cytosol.
(4) The HLA-6.0 or HLA-E genes may encode the lower molecular mass component on Jeg-3 cells, because these genes express molecules of about 38 kDa and 41 kDa, respectively, when transfected to appropriate host cells.
(5) In order to determine whether RA stimulates the hCG secretion by other trophoblastic cell lines, we evaluated the effect of RA on hCG, hCG-alpha subunit (hCG-alpha), and progesterone secretion in three choriocarcinoma cell lines: JEG-3, JAR, and BeWo.
(6) Reporter genes containing either the intact 1.5 kilobase of 5'-flanking sequence from the HCG alpha gene, or just the 36-bp tandem repeat cAMP response element, when coupled to an enhancerless promoter, both exhibit a vigorous cAMP stimulation of expression but no response to phorbol ester in JEG-3 cells.
(7) We demonstrate the molecular peculiarities of these modified smooth muscle cells using monoclonal antibodies produced against a benign human JEG-cell tumour.
(8) The transcriptional activities of P1 + P2 in their natural tandem orientation were additive in rat keratinocytes and human JEG choriocarcinoma cells.
(9) Using DNase I protection analyses and gel-mobility shift assays, we examined in vitro the domains in the human alpha-gene 5'-flanking sequence that bind nuclear proteins extracted from JEG-3 choriocarcinoma cells.
(10) The effect of JEG-3 choriocarcinoma supernatant on human natural killer cell and lymphokine-activated killer cell activity was investigated.
(11) Similar observations have been made in JeG-3 cells and Chinese hamster ovary cells stably transfected with human apoE cDNA constructs.
(12) In human choriocarcinoma JEG-3 and monkey COS-1 cells, the cytosolic thyroid hormone binding protein is a monomer of the tetrameric pyruvate kinase, subtype M2, which does not bind T3.
(13) In this study the 5'-flanking sequence of the CG beta gene was fused to a sensitive luciferase (LUC) reporter gene, allowing delineation of a CG beta CRE in transient expression assays performed in JEG-3 choriocarcinoma cells.
(14) JEG-3 choriocarcinoma cells demonstrate c-myc transcript levels that are greater than those of nonmalignant trophoblastic tissue at any stage of gestation.
(15) No significant expression of any of these ANG-GH fusion genes over the promoterless pOGH was observed in JEG-3 cells.
(16) Results of these studies suggest that there is some degree of tissue specificity of FN gene expression and reveal that cAMP induction is mediated, in part, by the same element (CRE) in both HT-1080 and JEG-3 cells.
(17) Epidermal growth factor (EGF) binds to JEG-3 cells, a tissue culture line of human choriocarcinoma.
(18) In JEG-3 choriocarcinoma cells the MMTV promoter is transcribed inefficiently, even after transfection of the receptor cDNA and treatment with glucocorticoids or progestins.
(19) A similar increase in Class I antigens is also found in JEG-3 choriocarcinoma cells after treatment with IFN-gamma.
(20) Deletion of the FN promoter from positions -510 to -56 resulted in an ~30-fold decrease in promoter activity when this construction was transfected into HT-1080 cells, and similar results were observed in NIH 3T3 cells; however, less than a 2-fold effect was observed in JEG-3 cells.
Veg
Definition:
Example Sentences:
(1) I choose the halibut fillet with scallops, dauphinoise potatoes, veg melange and pesto tapenade.
(2) Let’s begin just after the second world war, when Liverpool took a pre-season trip to the good ol’ US of A to gorge on meat, veg, malted milks and ice creams, working on the theory that by fattening themselves up, they’d have a season’s worth of energy stored when they got back to ration-book Britain.
(3) Groceries were delivered and a horse-drawn fruit and veg cart called along the road weekly.
(4) Use local, seasonal food The best restaurants observe the British seasons with their ingredients, so go to a farmers' market to buy your veg, and get the best you can afford.
(5) Anyway, swap fruit’n’veg, collect magic beans and compare your scores with Facebook friends.
(6) Then there are the "ethylene absorbing" discs you can place in a bowl to keep your fruit fresh for longer; the polyurethane foam cushions designed to prevent fruit and veg from becoming bruised; and the silicone "food huggers" into which you pop your leftover half a lemon or tomato.
(7) If you have a veg patch, you could be enjoying homegrown lettuce for most of the year.
(8) Saturday is a great day to sample its wines from France, Portugal, Spain and Italy as there is a weekly organic fruit and veg market outside.
(9) I'll do anything: peel spuds, look after the veg, make an impromptu pud.
(10) "I aim to do my seven a day without eating these processed things you are describing," says Oyinlola Oyebode, lead investigator of UCL's report , which argued the enhanced benefits of eating seven-plus portions of fruit and veg a day.
(11) You can buy some veg cheaper than supermarkets in some shops in my area.
(12) Vegetations were classified according to its size (longest axis) into small (veg less than 5 mm), medium (5 mm less than veg less than 10 mm) and large (veg less than 10 mm); according to its kind into "sessible" or "mobile" and according to its appearence into "cotton like" or "calcified".
(13) The privately owned chain is still a relative minnow, controlling just 5.8% of all grocery sales in the UK, but only Pampers nappies are bigger sellers than its Mamia brand, and 8% of our fresh fruit and veg, and over a fifth of all premium steaks, are bought in Aldi stores.
(14) Paris Cheapest for wine, onions Pricey for milk, lamb, bananas paris Freelance journalist Judith Prescott (above), who has lived in the French capital for two decades, shops at supermarket chain Auchan for her husband and two daughters, and reckons her bill is around €100 a week (£79), although that doesn't include the fruit and veg she tends to buy in local markets.
(15) A head of National Vegetarian Week we asked some of Britain's best veg-loving chefs for tips on how to jazz up meat-free cooking.
(16) • 2814 North 16 Street, barriocafe.com CULINARY HEROES FnB Facebook Twitter Pinterest Photograph: Jill Richards Photography Among the Native American souvenir stores of Scottsdale old town, is the excellent FnB, a truly forward-thinking, showcase for the state’s native produce in veg-centric dishes: salad of persimmons with hazelnut, kohlrabi, dill and goat’s cheese.
(17) Unlike Altmejd, she has always used materials that are resolutely downbeat: abandoned mattresses, old tights, fruit and veg, the toilet bowl.
(18) Aldi promises to be at least 15% cheaper than equivalent products at traditional supermarkets, and its weekly deals on fruit and veg, as well as fresh meat, from premium dry-cured steaks to basic mince, have fuelled a nationwide price war.
(19) The food, Korean, is cheap (starters less than a fiver; mains under £8) and very good: bibimbap, of course, also crisp fried mandu (dumplings) with homemade soy; tangy blanched and pickled veg; sizzling marinated beef; fat pork belly with garlic, kimchi and spicy doenjang sauce.
(20) Beetroot blueberry pancakes These bright pink pancakes liven up the breakfast table and are a great way to get some veg in the morning.