(1) Since the protective protein possesses extensive sequence homology with carboxypeptidase Y (carb Y) and the KEX 1 gene product from yeast, we have used the artificial substrate N-CBZ-Phe-Leu to detect and characterize the peptidase activity of the lysosomal carboxypeptidase (carb L).
(2) To study the molecular mechanisms involved in polyprotein processing, we have transfected the yeast KEX-2 gene into mouse NIH 3T3 fibroblasts and established a new cell line (called 2N-DK) where the KEX-2 endoprotease is permanently expressed.
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(4) The first-order rates are compared with that of a fourth variant Phe82----Gly which has been measured previously (E0 = 220 mV, kex = 0.7 s-1).
(5) While the mammalian endoproteases responsible for these cleavages are yet to be identified, this function has been unequivocally assigned in yeast to the product of the KEX-2 gene.
(6) The evidence for this cellular location is supported by measurement of intracellular and extracellular KEX-2 enzyme activity.
(7) The apparent pseudo-first-order exchange rate, kex, of the backbone amide protons and the correspondent activation enthalpies, delta H not equal to, were determined.
(8) For all other backbone amide protons in BPTI, the exchange was found to be uncorrelated and by an EX2 mechanism under all conditions of p2H and temperature where quantitative measurements could be obtained with the methods used, i.e., for kex approximately less than 5 min-1.
(9) For three variants of yeast iso-1-cytochrome c the rate constants measured by saturation transfer are wild-type (Asn52, E0 = 270 mV, kex = 0.3 s-1), Asn52----Ala (E0 = 240 mV, kex = 0.6 s-1), Asn52----Ile (E0 = 220 mV, kex = 1.0 s-1).
(10) It was found that the KEX-2 protease was unable to cleave the prepro-Lys-Arg-Pro-IL6 sequence, but that unspecific cleavage of the precursor protein had occurred.
(11) Thus, the kex phenotype does not require an alteration of the killer plasmid.--Kex1 and kex2 strains each contain near-normal levels of the 1.4 x 10(6) molecular weight double-stranded RNA, whose presence is correlated with the presence of the killer genome.
(12) The experimental study showed that KEX-I can inactivate directly the virus of Coxsackie B3, protect the heart cells in mice, prevent attack by Coxsackie B3, promote the growth of internal interferon and increase the NK cell's function to regulate immunity in experimental mice.
(13) When P2 organelles were separated in sucrose density gradients Rho1p comigrated with the Kex-2 activity, a late Golgi marker.
(14) Insulin affected both rate constants, reducing k(in) by 2.8-fold and increasing kex by 3.3-fold.
(15) The rate constants of efficient exchange interaction (kex) of spin-labelled lysozyme and the triplet probes perylene, eosine and anthracene butanoic acid with the haemoproteins were measured in microsomes and in solution by electron paramagnetic resonance and by the registration of delayed annihilation fluorescence.
(16) The look of Kex is, in part, down to Hálfdan Lárus Pedersen, a film production designer.
(17) At pH 6.0, first order rate constants for amino acid exit (kex) under steady state conditions for L-leucine, L-alanine, and L-serine were 1.1-1.3, 0.084, and 0.053 min-1, respectively.
(18) Most significantly, cleavage of the precursor to SRIF-28 was independent of the Kex 2 endoprotease since processing occurred efficiently in a kex 2 mutant strain.
(19) The clinical study was carried out with KEX-I according to a random, paired and cross-over design.
(20) Of particular clinical relevance is the [Cai] response to cardioplegia containing 16 mM [Kex]: neither group demonstrated the expected [Cai] increase in response to potassium depolarization.
Lex
Definition:
(n.) Law; as, lex talionis, the law of retaliation; lex terrae, the law of the land; lex fori, the law of the forum or court; lex loci, the law of the place; lex mercatoria, the law or custom of merchants.
Example Sentences:
(1) The Lex antigen was present in the void volume fraction of the majority (85%) of sera from adenocarcinoma patients.
(2) Murine liver extract (LEx) purified by ammonium sulfate (45-70% saturation) possesses a strong inhibitory effect on human lymphocyte proliferation.
(3) However, lesions with epithelial dysplasia showed H antigen on all spinous cells, and often also on basal cells, with expression of Lex and Ley restricted to the most superficial part of the epithelium above the H-positive cell layers.
(4) Expression of 4C9, a Lex[Gal beta 1----4(Fuc alpha 1----3)GlcNAc] antigen, during mouse embryogenesis was studied by immunohistochemical methods.
(5) A soluble alpha(1-3)-L-fucosyltransferase, which accepts carbohydrates of the general structure NeuAc alpha(2-3)Gal beta(1-4)GlcNAc beta-R as substrates and which is involved in the biosynthesis of the tumor-associated sialyl-LeX determinant, was purified about 125-fold from human amniotic fluid by a one-step affinity chromatography on fetuin-agarose.
(6) Cell surface expression of stage specific embryonic antigen 1 (SSEA-1), or Lex (III3 FucnLC4), was induced in differentiated human teratocarcinoma cells and in human diploid fibroblasts 3-6 d after infection with human cytomegalovirus (HCMV).
(7) In APs, staining with the four MoAbs recognizing extended LeX antigens correlated with the premalignant parameters of larger polyp size, more severe dysplasia, and increased villose component.
(8) Lex glycolipids were eluted from Protein A-silica columns over which plasma from patients with adenocarcinoma had previously been perfused.
(9) The oxygen affinity of human hemoglobin was determined from measurements of oxygen concentrations in equilibrated samples using a Lex-O2-Con apparatus (Lexington Instruments, Waltham, Mass.).
(10) It is suggested that in human pancreas, haptens T, Lex and Sialo-Lex, the oncodevelopmental cancer-associated antigens, are highly specific markers for malignancy and likely helpful in the early diagnosis of pancreatic cancer.
(11) We noted a linear relationship between the distance measured between two fixed points in excised bundles (Lex) and at the muscles' Lo.
(12) Hyperplastic ducts and ductules associated with pancreatic cancer expressed Lex-related antigens more frequently than morphologically similar lesions associated with chronic pancreatitis.
(13) In conclusion, extended LeX antigens and their sialylated derivatives are cancer-associated antigens that are expressed preferentially in premalignant colon polyps, that tend to correlate with malignant potential in these polyps, and that may eventually help to define mechanisms involved in the polyp-to-cancer sequence.
(14) Isolated polylactosaminylceramides exhibit I-, i-, H- and Lex antigenic activities which suggest that the polylactosaminylceramides are derived from both erythrocytes and granulocytes.
(15) The results have been analyzed in order to find out any possible association of cold autoanti-I antibodies, or cold alloantibodies, mainly anti-P1, anti-Leb, anti-Lea and anti-Lex, with certain diseases.
(16) Strains carrying lex, recA, or certain other combinations of mutations do not show any detectable phage-induced radioresistance.
(17) Immunohistochemical expression of blood group-related antigens (BGRAs), A, B, H, Leb, Lex and Ley was observed both in the cells in the culture, and in tumor transplanted into the pancreas.
(18) The hip-hop world has become dominated by styles such as drill and trap, and their preoccupation with drug dealing and womanising, with the purists' calls for a return to hip-hop's golden era drowned out by Lex Luger's snares and Gucci Mane 's endless chants of "burrrrr".
(19) We examined the distribution of blood group-related antigens using an indirect immunoperoxidase method with monoclonal antibodies (MAb) directed to A, B, H, Lewis a (Lea), Lewis b (Leb), Lewis x (Lex), and Lewis y (Ley) antigens and Type 1 precursor chain in human pancreas.
(20) The short- and the long-chain Lex antigens are significantly enhanced in colonic carcinoma.