(1) Since the protective protein possesses extensive sequence homology with carboxypeptidase Y (carb Y) and the KEX 1 gene product from yeast, we have used the artificial substrate N-CBZ-Phe-Leu to detect and characterize the peptidase activity of the lysosomal carboxypeptidase (carb L).
(2) To study the molecular mechanisms involved in polyprotein processing, we have transfected the yeast KEX-2 gene into mouse NIH 3T3 fibroblasts and established a new cell line (called 2N-DK) where the KEX-2 endoprotease is permanently expressed.
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(4) The first-order rates are compared with that of a fourth variant Phe82----Gly which has been measured previously (E0 = 220 mV, kex = 0.7 s-1).
(5) While the mammalian endoproteases responsible for these cleavages are yet to be identified, this function has been unequivocally assigned in yeast to the product of the KEX-2 gene.
(6) The evidence for this cellular location is supported by measurement of intracellular and extracellular KEX-2 enzyme activity.
(7) The apparent pseudo-first-order exchange rate, kex, of the backbone amide protons and the correspondent activation enthalpies, delta H not equal to, were determined.
(8) For all other backbone amide protons in BPTI, the exchange was found to be uncorrelated and by an EX2 mechanism under all conditions of p2H and temperature where quantitative measurements could be obtained with the methods used, i.e., for kex approximately less than 5 min-1.
(9) For three variants of yeast iso-1-cytochrome c the rate constants measured by saturation transfer are wild-type (Asn52, E0 = 270 mV, kex = 0.3 s-1), Asn52----Ala (E0 = 240 mV, kex = 0.6 s-1), Asn52----Ile (E0 = 220 mV, kex = 1.0 s-1).
(10) It was found that the KEX-2 protease was unable to cleave the prepro-Lys-Arg-Pro-IL6 sequence, but that unspecific cleavage of the precursor protein had occurred.
(11) Thus, the kex phenotype does not require an alteration of the killer plasmid.--Kex1 and kex2 strains each contain near-normal levels of the 1.4 x 10(6) molecular weight double-stranded RNA, whose presence is correlated with the presence of the killer genome.
(12) The experimental study showed that KEX-I can inactivate directly the virus of Coxsackie B3, protect the heart cells in mice, prevent attack by Coxsackie B3, promote the growth of internal interferon and increase the NK cell's function to regulate immunity in experimental mice.
(13) When P2 organelles were separated in sucrose density gradients Rho1p comigrated with the Kex-2 activity, a late Golgi marker.
(14) Insulin affected both rate constants, reducing k(in) by 2.8-fold and increasing kex by 3.3-fold.
(15) The rate constants of efficient exchange interaction (kex) of spin-labelled lysozyme and the triplet probes perylene, eosine and anthracene butanoic acid with the haemoproteins were measured in microsomes and in solution by electron paramagnetic resonance and by the registration of delayed annihilation fluorescence.
(16) The look of Kex is, in part, down to Hálfdan Lárus Pedersen, a film production designer.
(17) At pH 6.0, first order rate constants for amino acid exit (kex) under steady state conditions for L-leucine, L-alanine, and L-serine were 1.1-1.3, 0.084, and 0.053 min-1, respectively.
(18) Most significantly, cleavage of the precursor to SRIF-28 was independent of the Kex 2 endoprotease since processing occurred efficiently in a kex 2 mutant strain.
(19) The clinical study was carried out with KEX-I according to a random, paired and cross-over design.
(20) Of particular clinical relevance is the [Cai] response to cardioplegia containing 16 mM [Kex]: neither group demonstrated the expected [Cai] increase in response to potassium depolarization.
Vex
Definition:
(v. t.) To to/s back and forth; to agitate; to disquiet.
(v. t.) To make angry or annoyed by little provocations; to irritate; to plague; to torment; to harass; to afflict; to trouble; to tease.
(v. t.) To twist; to weave.
(v. i.) To be irritated; to fret.
Example Sentences:
(1) De Boer's successor's first tasks will be to keep the US aboard the negotiations and to clear up the vexed question of the legal status of the Copenhagen accord , the deal struck at Copenhagen by a small group but not endorsed by a majority of countries.
(2) There is also the vexed question of what should be the legal form of any Paris agreement, a subject likely to keep negotiators up late into the night at the conference, and some anxiety among the hosts over whether the text of a deal can be formulated in due time.
(3) But the bigger question, the one that has vexed historians, biographers and holocaust experts for eight decades, is why she was there.
(4) Cs (2 mM) reduced diastolic depolarization (DD) at different [Ca]O and in 10.8 mM [Ca]O revealed an oscillatory potential (VOS) and the decay of a prolonged depolarization (Vex).
(5) The past few days have been vexing ones for reporting guidelines, voluntary or legal.
(6) The present data also highlighted the vexed relationship between stress and seizure control, which needs to be further investigated.
(7) Another vexed national question in the coming months will be this one: who is the most worthy winner of BBC Sports Personality of the Year?
(8) Delivery of monoclonal antibodies to solid tumors is a vexing problem that must be solved if these antibodies are to realize their promise in therapy.
(9) Pathologists without considerable experience in the diagnosis of bone tumors find this question especially vexing.
(10) Caffeine (5 mM) abolishes Vos and Ios and increases Vex and Iex (as DOXO does), and adding DOXO slightly increased Vex and Iex.
(11) Posttraumatic joint stiffness is particularly vexing in the small joints.
(12) In this spirit, a vignette is offered from a clinical area in which questions of "health" and "illness" are particularly vexing at present.
(13) Some might argue that our eyes weren't quite on the ball back in '89: never mind the cataclysmic political upheaval in eastern Europe – the results of which still echo around the world – let's devote ourselves to a page concerned with vexed questions such as: why is water wet?
(14) The draft provides scant details on the vexed subject of accountability for emission reduction programmes.
(15) Nowhere was the commission’s balancing act more finely weighted than on the vexed question of bioenergy, which Cañete admitted was “a clear problem”.
(16) The top Chinese negotiator, Xie Zhenhua, said there was also a possibility of advances on the vexed issued of transparency – how to monitor, report and verify each nation's emissions to ensure they are honouring their pledges.
(17) But now it’s Isis who are the insurgents,” leaving the peshmerga with the vexing challenge of defending and holding territory.
(18) On the vexed issue of longer term finance, the Ethiopian prime minister Meles Zenawi presented an offer to reduce developing country demands by 75% to $100bn a year from 2020, in return for guarantees of how the money would be distributed.
(19) Discussed here are some contours of the vexing problem of adequate minority participation in the health professions and a brief discussion of some programs that appear to be working.
(20) After the creed and some Benjamin Britten, and a blessing and a long round of applause, the man charged with holding together the fractious global Anglican communion as it struggles with the vexed issues of women bishops and same-sex marriage processed out of the cathedral and into the bitterly cold spring afternoon.