(1) To facilitate the cleavage of fusion proteins, we have introduced a glycine-rich linker (glycine kinker) containing the sequence P.G.I.S.G.G.G.G.G located immediately following the thrombin cleavage site.
(2) 192, 262-267) which has a glycine-rich "kinker" immediately after the thrombin cleavage site.
(3) The introduction of the glycine kinker into fusion proteins allows for the cleavage of the fusion proteins while they are attached to the affinity resin resulting in a single step purification of the recombinant protein.
(4) We have constructed two new vectors, pGEX-KT and pGEX-KN, which have the glycine kinker placed N-terminal to the thrombin cleavage site in order to minimize the unrelated amino acids associated with the cleaved protein.
(5) This kinker dramatically improved the thrombin cleavage efficiency of several fusion proteins.
(6) The change in location of the kinker had no effect on the increased thrombin cleavage efficiency.
(7) A strategy combining the kinker in the vector pGEX-KN with polymerase chain reaction has also been developed to express fusion proteins which when cleaved with thrombin released a protein having no amino terminal extensions of any kind.
(8) This glycine kinker greatly increases the thrombin cleavage efficiency of several fusion proteins.
Skinker
Definition:
(n.) One who serves liquor; a tapster.
Example Sentences:
(1) Metathelazia Skinker, 1931 in which the cephalic structures could not be correlated with any other morphological or anatomical character.