(1) The transition was characterized by a single first order rate constant (kobs,i) which was independent of enzyme concentration.
(2) Knowledge of the salt dependences of ka and of the observed equilibrium constance Kobs of the ligand-nucleic acid interaction should usually be sufficient to determine whether a screening controlled mechanism or a pre-equilibrium mechanism is suitable to describe the process.
(3) A log kobs versus pH profile for the approach to the ionization equilibrium was determined, and a mechanism consistent with the profile was postulated.
(4) In the absence of cyanide, ferrocytochrome a3 appears at a rate (kobs) of 0.016 s-1.
(5) Above 25 microM AT.H complex, an increasing dead time displacement of p-aminobenzamidine and a downward deviation of kobs from the initial linear dependence on AT.H complex concentration were found, reflecting the saturation of an intermediate Xa.AT.H complex with a KD of 200 microM and a limiting rate of Xa-AT product complex formation of 140 s-1.
(6) At pH 7.8, the apparent single rate constant for association (kobs) at 4 degrees C was 4.72 x 10(+5).M-1.min-1.
(7) Thus, under pseudo-first order conditions ([AT]o, [H]o much greater than [T]o much less than [P]o), the observed thrombin inactivation rate constant (kobs) exhibited a saturable dependence on [AT]o or [H]o when [H]o much less than KT,H, reflecting a KAT,H (0.25 microM) similar to that directly determined by equilibrium binding.
(8) The nature of the anion often has a major effect on the magnitude of the equilibrium constant (Kobs) and rate constant (kobs) of protein-DNA interactions, but a minor effect on SKobs and Skobs, which are dominated by the cation stoichiometry.
(9) The rate constant (kobs) for loss of ADP activation exhibits a nonlinear dependence on 2-BDB-TAMP concentration, suggesting a reversible binding of reagent (KR = 0.74 mM) prior to irreversible modification.
(10) The kobs max value in tissues from insulin-dependent diabetic patients was significantly greater than that in tissues from either noninsulin-dependent diabetics or nondiabetics.
(11) Reduction by lumiflavin semiquinone followed single exponential kinetics and the observed rate constant (kobs) was linearly dependent on protein concentration (k = 1.8 X 10(7) M-1s-1 heme-1).
(12) At 0.25 M-KX, the increase in Kobs for Osym is observed to be approximately 40-fold, whereas for non-operator DNA the increase in Kobs is estimated by extrapolation to be approximately 300-fold.
(13) The Kobs of this reaction reflects the difference between the observed standard free energy change (delta G-oobs) for the hydrolysis of acetylcholine and the delta G-oobs for the hydrolysis of acetyl-CoA.
(14) The observed equilibrium constants (Kobs) of the creatine kinase (EC 2.7.3.2), myokinase (EC 2.7.4.3), glucose-6-phosphatase (EC 3.1.3.9), and fructose-1,6-diphosphatase (EC 3.1.3.11) reactions have been determined at 38 degrees C, pH 7.0, ionic strength 0.25, and varying free magnesium concentrations.
(15) Stopped-flow spectrophotometry also showed that electron transfer from the Fe-protein to the MoFe-protein in states E0 and E1H occurs at the same rate (kobs.
(16) A study of kobs vs pH suggests this active-site lysine has a pKa of 8.1 and a pH-independent rate constant of inactivation of 47,700 M-1 min-1.
(17) At all temperatures, the pH strongly influenced the observed degradation rate constant (kobs) values, with rate minima observed near pH 4.
(18) Alkylation of the N-terminal half resulted in biphasic calcium release with rates (kobs 153 s-1 and 10.9 s-1 respectively) similar to those observed in intact alkylated calmodulin.
(19) At 1.2 mM 2-BDB-TAMP, kobs = 0.060 min-1 and is not affected by alpha-ketoglutarate or GTP, but is decreased to 0.020 min-1 by 5 mM NADH and to zero by 5 mM ADP.
(20) A plot of the pseudo-first order rate constant (Kobs) versus T1K concentration was linear, and values for the association (k1) and dissociation (k-1) rate constants were obtained.
Sob
Definition:
(v. t.) To soak.
(v. i.) To sigh with a sudden heaving of the breast, or with a kind of convulsive motion; to sigh with tears, and with a convulsive drawing in of the breath.
(n.) The act of sobbing; a convulsive sigh, or inspiration of the breath, as in sorrow.
(n.) Any sorrowful cry or sound.
Example Sentences:
(1) "After I saw you there, I just went out and sobbed.
(2) The results suggest that (i) the SOS response of E. coli and the SOB response of B. subtilis are strikingly similar from both a phenotypic and a regulatory standpoint and that RecA and LexA protein analogs exist in B. subtilis, (ii) the Recbs protein is capable of regulating its own production, and (iii) SOS-inducing (RecA-activating) signals are generated in B. subtilis following either DNA damage or the development of physiological competence.
(3) Effects of amygdaloid lesions on the switch-off behavior (SOB) and behavioral changes induced by a delayed reinforcement (DR) for SOB were investigated in 12 cats.
(4) Acts of kindness move Langham to tears, and before long another memory has him sobbing.
(5) He went from minstrel show to blackface, from vaudeville to Broadway before he hit a fabulous prosperity as the most sentimental of all sentimental singers, a poor Russian cantor's son daubed with burnt cork and down on one knee sobbing for the "mammy" he had never known in a south that nobody ever knew.
(6) No one photographs the child with learning difficulty, sobbing as the teaching assistant they worked with for the past three years is booted out.
(7) He is very kind, honest, funny,” she said on Monday, sobbing as she remembered her only child, who had been flying home from Malaysia, where he was studying.
(8) In a televised meeting that has gone viral, the German chancellor rubs the shoulder of a sobbing teenager after telling her she was one of “thousands and thousands” of refugees that her country was unable to help.
(9) Since then, the cursing and sobbing have been plentiful.
(10) "This depressing morning has now got me questioning my pitiful existence," sobs James Dodge.
(11) She is generally a happy person, but in the last few weeks she has been showing signs of deep anxiety, phoning me sobbing with fear.
(12) The 56-year-old held a tissue to her face and sobbed during a five-minute hearing at City of Westminster magistrates court in central London.
(13) Liam Stacey , 21, of Pontypridd, south Wales, sobbed as he was taken away after the failed appeal hearing at Swansea crown court.
(14) The paper's "special investigation", headlined "No ID, no checks … and vouchers for sob stories: the truth behind those shock food bank claims", suggested that claims about the scale of Britain's welfare problems had been exaggerated.
(15) I sobbed for the last 30 pages but not, perhaps, for the reason you'd expect.
(16) Naturally I confronted them about it, halting their child's progress with a foot on the front bumper, loudly berating their crass behaviour while impressed pedestrians looked on, cheering and punching the air and chanting my name until Audi boy's parents fell to the ground, clutching pitifully at my trouser-legs and sobbing for forgiveness.
(17) 4.59pm BST "My fiancee have decided to get married in whichever country wins the World Cup so this game really has me torn," sobs Nate Philipps.
(18) She was followed by several women who must have been relatives or neighbours living nearby; the cries and sobs were so loud they could be heard clearly over the shooting and chanting from the street.
(19) "It's just so depressing this whole situation," sobs Angus Chisholm.
(20) One hand held the corner of the tomb and he sobbed uncontrollably into the other.