(1) The time course of the binding of a radioligand in the presence of a competing unlabeled ligand was analyzed in terms of rate constants of association (kon) and dissociation (koff) for binding of the radioligand and the competitor.
(2) Evidence is presented that the dependence of pH on koff for hDHFR accounts for the shape of the kcat versus pH curve for both hDHFR as well as its F31L variant and contributes to the higher efficiency of hDHFR compared with bacterial DHFR.
(3) In intact cells, the koff value for [3H]DTLET was lower than that found in membrane fractions in Krebs-HEPES and similar to that observed in membrane preparations in Tris-HCl supplemented with 30 mM NaCl.
(4) These results enable calculation of real time rate values for the enzyme-primer association (kon = 5.7 x 10(8) M-1 s-1) and dissociation (koff = 1.6 s-1).
(5) Dissociation kinetic studies indicated that the receptor types A, B, and C had different koff values allowing, therefore, a separate study of their binding properties.
(6) 3H-(-)-bupranolol associated to beta-adrenoceptors of lung membranes with a kon 1.21 X nmol-1 X min-1 and dissociated with a koff of 0.26 min-1.
(7) A temperature-dependence study of koff for the cortisol and progesterone complexes gave values of 0.0028 s-1 and 0.012 s-1 at 4 degrees C, respectively, and 0.88 s-1 and 4.5 s-1 at 37 degrees C, with progesterone dissociating about four to five times faster over the entire temperature range.
(8) One of three bound nucleotide molecules is localized at the site with Kd approximately 10(-7) M and released with koff approximately 0.1 s-1.
(9) The dissociation constant of the enzyme-inhibitor complex (koff) shows no pH dependence between pH 4 and pH 9.5.
(10) 2-(Trimethylammonio)ethyl pyrophosphate also inhibits with Ki' = 7 X 10(-7) M, kon' = 4.4 X 10(4) M-1 min-1, and koff = 0.03 min-1.
(11) The results of the inhibition of HL elastase by 175 benzoxazinones are reported herein with reference to hydrophobicity constants D, alkaline hydrolysis rates kOH-, inhibition constants Ki, and their component acylation and deacylation rate constants, kon and koff, respectively.
(12) In locust, 500 mM ethanol did not affect either Kon or Koff.
(13) The dissociation equilibrium constant of the binding was found to be 4 x 10(-9) M at 37 degrees C. Kinetic studies of complex formation and dissociation revealed that the kon and koff were 4 x 10(6) M-1 s-1 and 6.8 x 10(-2) s-1 respectively at 16 degrees C. Equilibrium competition experiments showed that the enzyme has varying affinities for a variety of nucleic acids in the order 23S rRNA greater than 16S rRNA greater than M13 DNA, f2 RNA greater than tRNA.
(14) These compounds are "slow-binding" inhibitors of HLE and, thus, allow the determination of Ki, the dissociation constant for the stable complex of inhibitor and enzyme, as well as kon and koff, the rate constants for formation and decomposition of this complex.
(15) We have treated hypospadia that is strictly glandular either with Duckett's Magpi procedure (54 cases, 49 long-term follow-up, 47 good results, retraction of the meatus in two), or Koff's advancement procedure if there is a grow in glans (13 cases, 12 good results, on requiring meatostomy).
(16) External pH effect was primarily mediated by modulation of kon; koff was again relatively unaffected.
(17) We measured the apparent individual on and off rate constants (kon and koff) for bFGF binding to these two sites on intact cells and to each class of binding site in the absence of the other.
(18) In the acidic form, koff was increased 5- and 20-fold when Arg 48 was replaced with Lys and Leu, respectively, while in the acidic form of mutants that possess Arg 48, koff was similar to that observed in CCP(MI).
(19) Butanol binds at the effector site but does not effect koff of TPB.
(20) For Cd(II) or Mg(II) at the B site in place of Zn(II), koff less than or equal to 1 s-1 X Cl-ion, which appears to coordinate the A site metal ion, enhances koff, suggesting that both Cl- and HPO2-4 can coordinate the A site metal ion in a 5-coordinate intermediate.
Vessel
Definition:
(n.) A hollow or concave utensil for holding anything; a hollow receptacle of any kind, as a hogshead, a barrel, a firkin, a bottle, a kettle, a cup, a bowl, etc.
(n.) A general name for any hollow structure made to float upon the water for purposes of navigation; especially, one that is larger than a common rowboat; as, a war vessel; a passenger vessel.
(n.) Fig.: A person regarded as receiving or containing something; esp. (Script.), one into whom something is conceived as poured, or in whom something is stored for use; as, vessels of wrath or mercy.
(n.) Any tube or canal in which the blood or other fluids are contained, secreted, or circulated, as the arteries, veins, lymphatics, etc.
(n.) A continuous tube formed from superposed large cylindrical or prismatic cells (tracheae), which have lost their intervening partitions, and are usually marked with dots, pits, rings, or spirals by internal deposition of secondary membranes; a duct.
(v. t.) To put into a vessel.
Example Sentences:
(1) Arterial compliance of great vessels can be studied through the Doppler evaluation of pulsed wave velocity along the arterial tree.
(2) With aging, the blood vessel wall becomes hyperreactive--presumably because of an augmented vasoconstrictor and a reduced vasodilator responsiveness.
(3) Multiple overlapping thin 3D slab acquisition is presented as a magnitude contrast (time of flight) technique which combines advantages from multiple thin slice 2D and direct 3D volume acquisitions to obtain high-resolution cross-sectional images of vessel detail.
(4) In the course of the syndrome development blood vessel permeability was increased in the anterior chamber of the eye.
(5) Aside from these characteristic findings of HCC, it was important to reveal the following features for the diagnosis of well differentiated type of small HCC: variable thickening or distortion of trabecular structure in association with nuclear crowding, acinar formation, selective cytoplasmic accumulation of Mallory bodies, nuclear abnormalities consisting of thickening of nucleolus, hepatic cords in close contact with bile ducts or blood vessels, and hepatocytes growing in a fibrous environment.
(6) Two fully matured specimens were collected from the blood vessel of two fish, Theragra chalcogramma, which was bought at the Emun market of Seoul in May, 1985.
(7) Its pathogenesis, still incompletely elucidated, involves the precipitation of immune complexes in the walls of the all vessels.
(8) In one of the cirrhotic patients, postmortem correlation of sonographic, angiographic, and pathological findings showed that the dilated vessels seen on sonography were cystic veins draining normally into the portal vein rather than portosystemic anastomoses.
(9) The observed pulmonary hypertension is probably the result of the left heart insufficiency and is being discussed with regard of the histopathological alterations in the heart muscle and the pulmonary vessels.
(10) DNA synthesis by endothelium subsequently increased and within 48 hr new blood vessel formation was detected.
(11) There was immediate resolution of paresthesia following mobilization of the impinging vessel from the nerve.
(12) After examining the cases reported in literature (Sacks, Barabas, Beighton Sykes), they point out that, contrary to what is generally believed, the syndrome is not rare and cases, sporadic or familial, of recurrent episodes of spontaneous rupture of the intestine and large vessels or peripheral arteries are frequent.
(13) The relationship between pressure at the functional site of origin of intracranial collateral channels (Pstem) and systemic pressure allows an estimation of the size of vascular channels from which collateral vessels originate.
(14) The release of possible peptide hormones into the interpeduncular cistern, where a pool of cerebrospinal fluid and large blood vessels occur, cannot be excluded.
(15) It is suggested that intra-endothelial conduction of electrical signals from capillaries to the resistance vessels may be involved in the local regulation of blood flow in the intact heart.
(16) Type C-like particles were found inter- and intracellularly in gland and vessel lumina and scattered in the connective tissue.
(17) We have characterized the effects of adenosine, the A1-receptor agonist N6-(L-2-phenylisopropyl)-adenosine (PIA) and the A2-receptor agonist 5'-(N-ethyl)-carboxamido-adenosine (NECA), in isolated human pulmonary vessels.
(18) It appears that the viscosity of the arterial wall must be the major source of attenuation in the larger arteries, while the viscosity of the blood plays a significant role only in the smaller vessels.
(19) In the choroid, VIP-immunoreactive fibers were seen mainly in close association with the choroidal blood vessels.
(20) Resistance vessels play a predominant role in limiting systemic arterial pressure in the orthostatic position.