(n.) A long, slender rope made of hemp or strips of hide, esp. one with a noose; -- used as a lasso for catching cattle, horses, etc., and for picketing a horse so that he can graze without wandering.
(v. t.) To secure with a lariat fastened to a stake, as a horse or mule for grazing; also, to lasso or catch with a lariat.
Example Sentences:
(1) The RNA-catalysed self-splicing reaction of group II intron RNA is assumed to proceed by two consecutive transesterification steps, accompanied by lariat formation.
(2) Two other discrete RNA species produced during splicing in vitro may represent reaction intermediates: free, linear exon 1 and a form of the intron lariat extending beyond the 3' splice site to include exon 2.
(3) Specifically, StAg splicing uses either of two lariat branch points, one of which is located only 4 nucleotides from the 3' splice site.
(4) The deoxyribose rings towards the 5' terminus exist predominantly in the S form (2'-endo-3'-exo) while residues on or adjacent to the 2' branch site in the eventual lariat structure [A(6) of TACTAAC] show more N-character (3'endo-2'-exo).
(5) Third, the lariat IVS is linearized after the release of the small RNP.
(6) Fewer branches were found in the poly A(-) RNAs of the nucleus and of late adenovirus transcripts suggesting that excised lariat introns do not accumulate in vivo.
(7) The internal exon was missing from the spliced product and together with two flanking introns was included in a large lariat structure.
(8) We also find linear IVS1 and a randomly nicked lariat, the latter probably being made during RNA isolation.
(9) The site of branchpoint formation was localized by debranching the Drosophila lariat with mammalian (HeLa) cell debranching enzyme and by P1 and T2 nuclease analysis.
(10) Processing of dead-end lariat intermediates to mRNA correlates with base pairing between U5 and the first two bases in exon 2.
(11) First, the pre-mRNA is cleaved at the 5' splice site and a branched (lariat) intermediate is formed.
(12) Here we demonstrate that 5' SS cleavage and lariat formation take place in vitro in the absence of the 3' SS and much of the 3' junction.
(13) Sequence analysis of these genomic clones revealed that the two short segments specific for the two mRNAs are tandemly arranged in a genomic sequence and form exonic sequences equipped with AG and GT sequences on their 5' and 3' ends, respectively, and the putative consensus sequences for the lariat formation.
(14) Furthermore, incubation of oocyte RNA with a HeLa cell extract with lariat debranching activity converted the small RNA to a class that now migrated as less than or equal to 140 nucleotides in length in 8% gels, consistent with the size of the linear intraleader intron.
(15) These results suggest that during the normal in vitro splicing reaction the 2',5'-phosphodiester bond of RNA lariats is protected from cleavage by the lariat debranching activity.
(16) Addition of fraction Ia to the combination of Ib and II resulted in the formation of spliced RNA and the intron lariat.
(17) The incubation of two of the fractions (Ib and II) in the presence of ATP resulted in cleavage of precursor mRNA at the 5' splice site and formation of the intron-exon lariat.
(18) Pre-mRNAs lacking the authentic branch point sequence are accurately spliced in vitro; processing of the mutant pre-mRNAs generates RNA lariats due to the activation of cryptic branch points within IVS1.
(19) In addition, the data strongly reinforce the importance of the distance constraint for lariat formation.
(20) Instead of inching my way along a busy B-road in the drizzle, wearing a hard hat and a hi-vis jacket, I was on a black-and-white pony in the wild west, riding alongside men with names like Cody who talked kinda slow and carried lariats on their saddles.