(n.) A long, slender rope made of hemp or strips of hide, esp. one with a noose; -- used as a lasso for catching cattle, horses, etc., and for picketing a horse so that he can graze without wandering.
(v. t.) To secure with a lariat fastened to a stake, as a horse or mule for grazing; also, to lasso or catch with a lariat.
Example Sentences:
(1) The RNA-catalysed self-splicing reaction of group II intron RNA is assumed to proceed by two consecutive transesterification steps, accompanied by lariat formation.
(2) Two other discrete RNA species produced during splicing in vitro may represent reaction intermediates: free, linear exon 1 and a form of the intron lariat extending beyond the 3' splice site to include exon 2.
(3) Specifically, StAg splicing uses either of two lariat branch points, one of which is located only 4 nucleotides from the 3' splice site.
(4) The deoxyribose rings towards the 5' terminus exist predominantly in the S form (2'-endo-3'-exo) while residues on or adjacent to the 2' branch site in the eventual lariat structure [A(6) of TACTAAC] show more N-character (3'endo-2'-exo).
(5) Third, the lariat IVS is linearized after the release of the small RNP.
(6) Fewer branches were found in the poly A(-) RNAs of the nucleus and of late adenovirus transcripts suggesting that excised lariat introns do not accumulate in vivo.
(7) The internal exon was missing from the spliced product and together with two flanking introns was included in a large lariat structure.
(8) We also find linear IVS1 and a randomly nicked lariat, the latter probably being made during RNA isolation.
(9) The site of branchpoint formation was localized by debranching the Drosophila lariat with mammalian (HeLa) cell debranching enzyme and by P1 and T2 nuclease analysis.
(10) Processing of dead-end lariat intermediates to mRNA correlates with base pairing between U5 and the first two bases in exon 2.
(11) First, the pre-mRNA is cleaved at the 5' splice site and a branched (lariat) intermediate is formed.
(12) Here we demonstrate that 5' SS cleavage and lariat formation take place in vitro in the absence of the 3' SS and much of the 3' junction.
(13) Sequence analysis of these genomic clones revealed that the two short segments specific for the two mRNAs are tandemly arranged in a genomic sequence and form exonic sequences equipped with AG and GT sequences on their 5' and 3' ends, respectively, and the putative consensus sequences for the lariat formation.
(14) Furthermore, incubation of oocyte RNA with a HeLa cell extract with lariat debranching activity converted the small RNA to a class that now migrated as less than or equal to 140 nucleotides in length in 8% gels, consistent with the size of the linear intraleader intron.
(15) These results suggest that during the normal in vitro splicing reaction the 2',5'-phosphodiester bond of RNA lariats is protected from cleavage by the lariat debranching activity.
(16) Addition of fraction Ia to the combination of Ib and II resulted in the formation of spliced RNA and the intron lariat.
(17) The incubation of two of the fractions (Ib and II) in the presence of ATP resulted in cleavage of precursor mRNA at the 5' splice site and formation of the intron-exon lariat.
(18) Pre-mRNAs lacking the authentic branch point sequence are accurately spliced in vitro; processing of the mutant pre-mRNAs generates RNA lariats due to the activation of cryptic branch points within IVS1.
(19) In addition, the data strongly reinforce the importance of the distance constraint for lariat formation.
(20) Instead of inching my way along a busy B-road in the drizzle, wearing a hard hat and a hi-vis jacket, I was on a black-and-white pony in the wild west, riding alongside men with names like Cody who talked kinda slow and carried lariats on their saddles.
Tether
Definition:
(n.) A long rope or chain by which an animal is fastened, as to a stake, so that it can range or feed only within certain limits.
(v. t.) To confine, as an animal, with a long rope or chain, as for feeding within certain limits.
Example Sentences:
(1) A comparison was made between the Q's estimated by the CO2 rebreathing method during tethered swimming and previously published data on Q determined by the dye-dilution method during free swimming in a flune.
(2) In both conditions about half the number of cases presented clinically before the age of 3 months, when paraplegia was evident in only 5 of the 26 infants with tethered cord and 3 of the 25 with tethered roots.
(3) Alternatively, a loss of collagen tethers or decline in matrix tensile strength can be responsible for regional or global transformations in myocardial architecture and function seen in the reperfused ("stunned") myocardium and in dilated (idiopathic) cardiopathy.
(4) The hypothesis that a localized lordosis, or tethering of the posterior elements of the spine, is the primary cause of the vertebral rotation in idiopathic scoliosis was investigated in anatomic specimens of human and calf spinal columns.
(5) The ultrasound-induced negative phonotactic response of tethered, flying Australian field crickets habituates to repeated stimuli.
(6) In one patient, the fibrous band extended from the distal pole of the patella to the intracondylar notch, tethering the patella inferiorly.
(7) Single-chain antigen-binding proteins are novel recombinant polypeptides, composed of an antibody variable light-chain amino acid sequence (VL) tethered to a variable heavy-chain sequence (VH) by a designed peptide that links the carboxyl terminus of the VL sequence to the amino terminus of the VH sequence.
(8) We have described delayed presentation in an adult with evidence of the tethered spinal cord syndrome following myelomeningocele repair.
(9) We report 2 cases of the tethered spinal cord syndrome.
(10) Internal motions of the tethered Er-labeled ligands may also contribute to the observed anisotropy decay, particularly in the case of cell-bound IgE.
(11) Computerized interpretation of the electrocardiogram has now advanced to computerization of the electrocardiograph, resulting in greatly increased versatility, including the capacity for adapting to a variety of lead systems rather than being tethered to the old Einthoven-Wilson-Goldberger (EWG) system.
(12) Apple was asked to comment on this ability to unlock the iPhone tethering facility for free.
(13) Three cases of tethered cord syndrome are presented with reference of reported literatures.
(14) However visitors to benm.at – an iPhone and iPod touch enthusiasts' website – can download a profile that instantly activates the tethering system free of charge.
(15) A 14-residue peptide based on the tethered ligand stimulated the aggregation of gel-filtered platelets with an EC50 of 7 microM, and a concentration of 10 microM was the minimum concentration necessary to yield a full aggregation response in platelet-rich plasma.
(16) The ratio of the change in the length of the tether to the change in the projection length is proportional to the ratio of the pipette radius to the tether radius.
(17) Surgical repair with release of cord tethering at the time of diagnosis is advocated, regardless of patient age.
(18) In single-channel recordings, tethered agonists opened channels with the same conductance as reversibly bound agonists (30 pS at 15 degrees C and -100 mV).
(19) In a series of 75 patients with surgically treated lipomyelomeningoceles, the neurological condition of six patients deteriorated 6 months to 14 years after the operation due to repeat tethering of the spinal cord.
(20) Failure to release this structure from the proximal ulna caused kinking and tethering of the nerve when transposition was attempted.